Part of Thermo Fisher Scientific

Collagen I Monoclonal Antibody (5D8-G9/Col1)

Collagen I Antibody for ELISA, IHC (F), Western blot

Synonyms:
COL1, COL1A2
Entrez Gene ID:
(Human) 420564
UniProt ID:
P02467
Details
Host / Isotype: Mouse / IgG1, kappa
Class: Monoclonal
Type: Antibody
Clone: 5D8-G9/Col1
Species Reactivity: Human (Hu) Goat (Gt) Canine (Ca) Bovine (Bv) Ovine (Ov) Porcine (Po)
Immunogen: Pepsin-solublized, salt-fractioned type I collagen from human placenta
Ordering Information
Pierce Collagen I Monoclonal Antibody (5D8-G9/Col1)
Product Number Pkg. Size Price Purchase
CSI 008-01-02 200 µg $543.00
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Storage: 4º C, store in dark
Form: 200 µg of Protein A purified antibody (1 mg/ml) in 0.01 M phosphate buffer, with 0.5 M NaCl and 15 mM sodium azide; pH 7.4.


Applications Dilution *
ELISA (ELISA) 1:16,000
Immunohistochemistry (Frozen) (IHC (F)) Assay dependent
Western Blot (WB) Assay dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
CSI 008-01-02 is highly specific for type I collagen. It has been shown to have no cross-reactivity with type II, III, V and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connnective tissue proteins (laminin, fibronectin, elastin). CSI 008-01-02 reacts with human, calf, sheep, pig, dog and goat type I collagens by ELISA and immunoblotting. No reactivity with mouse, rat, guinea pig, cat, rabbit, squirrel, horse, kangaroo or chicken type I collagen by ELISA and immunoblotting. CSI 008-01-02 has a Ka of approximately 2.5 x 10^6 for human type I collagen.
 
CSI 008-01-02 has been successfully used in Western blotting, ELISA, and immunohistochemistry (frozen) procedures. In immunoblotting CSI 008-01 detects human type I collagen only in its native triple helix form. CSI 008-01 has been used successfully for immunohistology on frozen unfixed sections of human, and bovine skin, and for identification of collagen synthesis in cell culture. If fixation of tissue is required, acetone or ethanol is recommended.

The specificity of the CSI 008-01-02 epitope is located near the C-terminal of the molecule.

The CSI 008-01-02 immunogen is pepsin-solublized, salt-fractioned type I collagen from human placenta.
General Information
Type I collagen is present in soft connective tissue and bone where it represents approximately 90% of the organix matrix. Type I collagen is produced from procollagen when two teloterminal propeptides are digested from the procursor and released into the circulation. Defects in one or both alleles of the type I collagen gene causes “brittle bone” disorder (osteogenesis imperfecta). Patients suffering from such defects have low levels of fully functional type I collagen resulting in bones that break very easily.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)