Cdk5 Antibody (DC34)

Cdk5 Monoclonal Antibody for Western blot, IF, ICC, IP

>> See 7 other antibodies for CDK5 (TPKII catalytic subunit, cell division protein kinase 5, protein kinase CDK5 splicing, serine/threonine-protein kinase PSSALRE, tau protein kinase II catalytic subunit)
Synonyms:
TPKII catalytic subunit, cell division protein kinase 5, protein kinase CDK5 splicing, serine/threonine-protein kinase PSSALRE, tau protein kinase II catalytic subunit
Entrez Gene ID:
Details
Host / Isotype: Mouse / IgG1, kappa
Class: Monoclonal
Type: Antibody
Clone: DC34
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Published Species Reactivity: Rat (Rt)
Immunogen: Recombinant cdk5 protein
Ordering Information
Pierce Cdk5 Antibody (DC34)
Product #
MA5-14366
Size
500 µl
Price
$425.00
Purchase
Add Cdk5 Antibody (DC34) to your cart.
Tested Applications Dilution *
Western Blot (WB) Assay Dependent
Immunofluorescence (IF) 1:10-1:100
Immunocytochemistry (ICC) 1:10-1:100
Immunoprecipitation (IP) 2 µg/mg protein lysate
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.2mg/ml
Purification: Protein G
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C
Product Specific Information
MA5-14366 targets Cdk5 in immunofluorescence, immunoprecipitation, and Western blot applications and shows reactivity with human, mouse, and rat samples.

The MA5-14366 immunogen is recombinant cdk5 protein.
General Information
Cdk5 is expressed in both cycling and noncycling cells and is present in numerous mammalian tissues. Neuronal and muscle cells contain the highest amount of this protein. The p35 protein, which is expressed solely in the brain, activates Cdk5. Cdk5 activity is involved in terminal differentiation of neurons and muscle cells.
Product Images
  • Cdk5 Antibody (MA5-14366) in WB
    MA5-14366_WesternBlot.jpg

    Western Blot with anti-Cdk5 Monoclonal Antibody [DC34] (MA5-14366) (MA5-14366_WesternBlot.jpg)

    Western Blot with anti-Cdk5 Monoclonal Antibody [DC34] (MA5-14366)

    Western blot of Cdk5 using Cdk5 Monoclonal Antibody (MA5-14366) on MDA231 Cells, MAD109 Cells and CCL-4 med Cells.
  • Cdk5 Antibody (MA5-14366) in IF
    MA5-14366_IF_Jurkat_20131018125519.jpg

    Immunofluorescence with anti-Cdk5 Monoclonal Antibody [DC34] (MA5-14366) (MA5-14366_IF_Jurkat_20131018125519.jpg)

    Immunofluorescence with anti-Cdk5 Monoclonal Antibody [DC34] (MA5-14366)

    Immunofluorescent analysis of Cdk5 (green) showing staining in the cytoplasm of Jurkat cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cdk5 monoclonal antibody (Product # MA5-14366) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • Cdk5 Antibody (MA5-14366) in IP
    MA5-14366_Immunoprecipitation.jpg

    Immunoprecipitation with anti-Cdk5 Monoclonal Antibody [DC34] (MA5-14366) (MA5-14366_Immunoprecipitation.jpg)

    Immunoprecipitation with anti-Cdk5 Monoclonal Antibody [DC34] (MA5-14366)

    Immunoprecipitation of Cdk5 using Cdk5 Monoclonal Antibody (MA5-14366) on Native Human LS174T Cells.
Publications:
Western Blot
  Species / Dilution Summary
  Rt / 1:1000
MA5-14366 was used in immunocytochemistry and western blot to study the role of Cdk5 in the mechanism by which lithium exerts its neuroprotective effects

Neuroscience. 2005;134(3):1001-11.
"Implication of cyclin-dependent kinase 5 in the neuroprotective properties of lithium."
Author(s): Jordà EG, Verdaguer E, Canudas AM, Jiménez A, Garcia de Arriba S, Allgaier C, Pallàs M, Camins A
Number of Citations: 8
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Rt / 1:400
MA5-14366 was used in immunocytochemistry and western blot to study the role of Cdk5 in the mechanism by which lithium exerts its neuroprotective effects

Neuroscience. 2005;134(3):1001-11.
"Implication of cyclin-dependent kinase 5 in the neuroprotective properties of lithium."
Author(s): Jordà EG, Verdaguer E, Canudas AM, Jiménez A, Garcia de Arriba S, Allgaier C, Pallàs M, Camins A
Number of Citations: 8
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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