Cdk5 Antibody (DC17 + DC34)

Cdk5 Monoclonal Antibody for Western blot, IF, IP

>> See 7 other antibodies for CDK5 (TPKII catalytic subunit, cell division protein kinase 5, protein kinase CDK5 splicing, serine/threonine-protein kinase PSSALRE, tau protein kinase II catalytic subunit)
Synonyms:
TPKII catalytic subunit, cell division protein kinase 5, protein kinase CDK5 splicing, serine/threonine-protein kinase PSSALRE, tau protein kinase II catalytic subunit
Entrez Gene ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: DC17 + DC34
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Published Species Reactivity: Rat (Rt)
Immunogen: Recombinant cdk5 protein
Ordering Information
Pierce Cdk5 Antibody (DC17 + DC34)
Product #
MA5-11291
Size
500 µl
Price
$330.00
Purchase
Add Cdk5 Antibody (DC17 + DC34) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1-2 µg/ml
Immunofluorescence (IF) Assay Dependent
Immunoprecipitation (IP) 6 ug/mg protein lysate
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.2mg/ml
Purification: Protein G
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C
Product Specific Information
MA5-11291 targets Cdk5 in IF, IP, and WB applications and shows reactivity with Human, mouse, and Rat samples.

The MA5-11291 immunogen is recombinant cdk5 protein.
General Information
Cdk5 is expressed in both cycling and noncycling cells and is present in numerous mammalian tissues. Neuronal and muscle cells contain the highest amount of this protein. The p35 protein, which is expressed solely in the brain, activates Cdk5. Cdk5 activity is involved in terminal differentiation of neurons and muscle cells.
Product Images
  • Cdk5 Antibody (MA5-11291) in WB
    82338.jpg

    Western Blot with anti-Cdk5 Monoclonal Antibody [DC17 + DC34] (MA5-11291) (82338.jpg)

    Western Blot with anti-Cdk5 Monoclonal Antibody [DC17 + DC34] (MA5-11291)

    HeLa cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ONTARGETplus CDK5 siRNA (Lane 3). Western blot data for GAPDH is included as a control for equal protein loading.
  • Cdk5 Antibody (MA5-11291) in WB
    MA5-11291_WesternBlot.jpg

    Western Blot with anti-Cdk5 Monoclonal Antibody [DC17 + DC34] (MA5-11291) (MA5-11291_WesternBlot.jpg)

    Western Blot with anti-Cdk5 Monoclonal Antibody [DC17 + DC34] (MA5-11291)

    Western blot of Cdk5 using Cdk5 Monoclonal Antibody (MA5-11291) on IMR-5 Cells.
  • Cdk5 Antibody (MA5-11291) in IP
    MA5-11291_Immunoprecipitation.jpg

    Immunoprecipitation with anti-Cdk5 Monoclonal Antibody [DC17 + DC34] (MA5-11291) (MA5-11291_Immunoprecipitation.jpg)

    Immunoprecipitation with anti-Cdk5 Monoclonal Antibody [DC17 + DC34] (MA5-11291)

    Immunoprecipitation of Cdk5 using Cdk5 Monoclonal Antibody (MA5-11291) on Native Human LS174T Cells.
  • Cdk5 Antibody (MA5-11291) in IP
    MA5-11291_IP_Jurkat.jpg

    Immunoprecipitation with anti-Cdk5 Monoclonal Antibody [DC17 + DC34] (MA5-11291) (MA5-11291_IP_Jurkat.jpg)

    Immunoprecipitation with anti-Cdk5 Monoclonal Antibody [DC17 + DC34] (MA5-11291)

    Immunoprecipitation and Western blot of Cdk5 was performed using Jurkat cell lysates. Cells were lysed using ice cold IP lysis/wash buffer and pre-cleared using 50 ml of bead slurry per ml of cell lysate. Antigen-antibody complexes were formed by incubating 0.5 ml pre-cleared cell lysate on ice for 3hrs with 8-15ug of Cdk5 monoclonal antibody (Product # MA5-11291) crosslinked to Protein A/G plus agarose. The immune complexes were eluted using 60 ul sample buffer boiled at 95ºC for 5 min and loaded onto an SDS-PAGE gel; Input (lane 1), Jurkat IP (Lane 2) and Jurkat supernatant (lane 3). The membrane was probed with a Cdk5 monoclonal antibody (Product # MA5-11291) at a dilution of 6ug/mg of lysate followed by detection using an HRP-conjugated goat anti-mouse IgG + IgM (H+L) cross-adsorbed secondary antibody. Chemiluminescent detection was performed using an exposure time of 20s, resulting in a ~30kDa band on input and IP lysates.
Publications:
Western Blot
  Species / Dilution Summary
  Rt / 1:1000
MA5-11291 was used in immunocytochemistry and western blot to study the role of Cdk5 in the mechanism by which lithium exerts its neuroprotective effects

Neuroscience. 2005;134(3):1001-11.
"Implication of cyclin-dependent kinase 5 in the neuroprotective properties of lithium."
Author(s): Jordà EG, Verdaguer E, Canudas AM, Jiménez A, Garcia de Arriba S, Allgaier C, Pallàs M, Camins A
Number of Citations: 8
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Rt / 1:400
MA5-11291 was used in immunocytochemistry and western blot to study the role of Cdk5 in the mechanism by which lithium exerts its neuroprotective effects

Neuroscience. 2005;134(3):1001-11.
"Implication of cyclin-dependent kinase 5 in the neuroprotective properties of lithium."
Author(s): Jordà EG, Verdaguer E, Canudas AM, Jiménez A, Garcia de Arriba S, Allgaier C, Pallàs M, Camins A
Number of Citations: 8
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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