Part of Thermo Fisher Scientific

Cdc2 Monoclonal Antibody (A17)

Cdc2 Antibody for ELISA, ICC, IF, IHC (P, F), IP, RIA, Western blot

Synonyms:
CDK1, DKFZp686L20222, MGC111195
Entrez Gene ID:
(Human) 983, (Murine) 12534
UniProt ID:
P06493
Details
Host / Isotype: Mouse / IgG2a
Class: Monoclonal
Type: Antibody
Clone: A17
Species Reactivity: Human (Hu) Murine (Ms) Rat (Rt) Chicken (Ck) Amphibian (Am) Xenopus laevis (Xl)
Immunogen: Carboxyterminal fragment starting at aa 85 of Xenopus p34cdc2 expressed in E. coli
Ordering Information
Pierce Cdc2 Monoclonal Antibody (A17)
Product Number Pkg. Size Price Purchase
MA1-21453 100 µg $354.00
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Storage: 4º C
Form: 100 µg of Protein A purified antibody (1.2 mg/ml) in PBS


Applications Dilution *
Immunocytochemistry (ICC) 5 µg/ml
Western Blot (WB) 1µg/ml
Immunofluorescence (IF) Assay dependent
Immunohistochemistry (Paraffin, Frozen) (IHC (P, F)) 5 µg/ml
ELISA (ELISA) Assay dependent
Immunoprecipitation (IP) 2.5 µg/I
Radioimmune Assays (RIA) Assay dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA1-21453 detects Cdc2 (CDK1) in human, rat, mouse, chicken, xenopus laevis, and amphibian samples.

MA1-21453 has been successfully used in ELISA, immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation, radioimmune assays, and Western Blot procedures.

MA1-21453 immunogen corresponds to Carboxyterminal fragment starting at aa 85 of Xenopus p34cdc2 expressed in E. coli.

Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
General Information
Cdc2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor, that induces entry into mitosis and is universal among eukaryotes. In the fission yeast Schizosaccharomyces pombe, the gene Cdc2 is responsible for controlling the transition from G1 phase to the S phase and from the G2 phase to the M phase of the cell cycle. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34-cyclin B complex that is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)