Caveolin 3 Antibody

Caveolin 3 Polyclonal Antibody for Western blot, IF, ICC, IHC, IP

>> See 2 other antibodies for CAV3 (CAV3, CAV, M-caveolin)
Synonyms:
CAV3, CAV, M-caveolin
Entrez Gene ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Published Species Reactivity: Mouse (Ms), Rat (Rt)
Immunogen: Synthetic peptide corresponding to residues M(1) M T E E H T D L E A R I I K D I H C(19) C of mouse CAV3
Ordering Information
Pierce Caveolin 3 Antibody
Product #
PA1-066
Size
100 µg
Price
$360.00
Purchase
Add Caveolin 3 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1µg/ml
Immunofluorescence (IF) 1:20
Immunocytochemistry (ICC) Assay dependent
Immunohistochemistry (IHC) 1:100-200
Immunoprecipitation (IP) Assay dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-066 detects Caveolin 3 from rat and mouse tissues. This antibody does not detect caveolin-1 or -2.

PA1-066 has been successfully used in Western blot, IF, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~21 kDa protein representing Caveolin 3 from rat heart homogenate.

The PA1-066 immunogen is a synthetic peptide corresponding to residues M(1) M T E E H T D L E A R I I K D I H C(19) C of mouse CAV3. This sequence is ~85% conserved in human Caveolin 3. PA1-066 immunizing peptide (Cat. # PEP-081) is available for use in neutralization and control experiments.
General Information
Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin gene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissues.
Product Images
  • Caveolin 3 Antibody (PA1-066) in WB
    PA1-066_1_07232010.jpg

    Western Blot with anti-Caveolin 3 Polyclonal Antibody (PA1-066) (PA1-066_1_07232010.jpg)

    Western Blot with anti-Caveolin 3 Polyclonal Antibody (PA1-066)

    Western blot detection of CAV3 from rat cardiac muscle protein extract using PA1-066.
  • Caveolin 3 Antibody (PA1-066) in IF
    PA1-066_IF_C2C12.jpg

    Immunofluorescence with anti-Caveolin 3 Polyclonal Antibody (PA1-066) (PA1-066_IF_C2C12.jpg)

    Immunofluorescence with anti-Caveolin 3 Polyclonal Antibody (PA1-066)

    Immunofluorescent analysis of Caveolin 3 in C2C11 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Caveolin 3 polyclonal antibody (Product # PA1-066) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Caveolin 3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
  • Caveolin 3 Antibody (PA1-066) in IF
    PA1-066_IF_Hela.jpg

    Immunofluorescence with anti-Caveolin 3 Polyclonal Antibody (PA1-066) (PA1-066_IF_Hela.jpg)

    Immunofluorescence with anti-Caveolin 3 Polyclonal Antibody (PA1-066)

    Immunofluorescent analysis of Caveolin 3 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Caveolin 3 polyclonal antibody (Product # PA1-066) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Caveolin 3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
  • Caveolin 3 Antibody (PA1-066) in IHC
    PA1-066_IHC_MS_heart_tissue.jpg

    Immunohistochemistry with anti-Caveolin 3 Polyclonal Antibody (PA1-066) (PA1-066_IHC_MS_heart_tissue.jpg)

    Immunohistochemistry with anti-Caveolin 3 Polyclonal Antibody (PA1-066)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse heart tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing Caveolin 3 (PA1-066) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Caveolin 3 Antibody (PA1-066) in IHC
    PA1-066_IHC_MS_lymphnode_tissue.jpg

    Immunohistochemistry with anti-Caveolin 3 Polyclonal Antibody (PA1-066) (PA1-066_IHC_MS_lymphnode_tissue.jpg)

    Immunohistochemistry with anti-Caveolin 3 Polyclonal Antibody (PA1-066)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse lymph node tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing Caveolin 3 (PA1-066) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Caveolin 3 Antibody (PA1-066) in IHC
    PA1-066_IHC_MS_skeletal_muscle_tissue.jpg

    Immunohistochemistry with anti-Caveolin 3 Polyclonal Antibody (PA1-066) (PA1-066_IHC_MS_skeletal_muscle_tissue.jpg)

    Immunohistochemistry with anti-Caveolin 3 Polyclonal Antibody (PA1-066)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Caveolin 3 (PA1-066) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Ms / 1:1,000
PA1-066 was used in western blot to investigate the effect of caveolin on glycosylphosphatidyl inositol-linked protein function .

Mol Cell Biol. 2002 Jun;22(11):3905-26.
"Intracellular retention of glycosylphosphatidyl inositol-linked proteins in caveolin-deficient cells."
Author(s): Sotgia F, Razani B, Bonuccelli G, Schubert W, Battista M, Lee H, Capozza F, Schubert AL, Minetti C, Buckley JT, Lisanti MP
Number of Citations: 15
(See PubMed article )
  Ms / Not Cited
PA1-066 was used in western blot to study the role of muscle caveolae in the control of energy metabolism in skeletal muscle fibers.

Am J Pathol. 2003 Dec;163(6):2619-34.
"Phosphofructokinase muscle-specific isoform requires caveolin-3 expression for plasma membrane recruitment and caveolar targeting: implications for the pathogenesis of caveolin-related muscle diseases."
Author(s): Sotgia F, Bonuccelli G, Minetti C, Woodman SE, Capozza F, Kemp RG, Scherer PE, Lisanti MP
Number of Citations: 1
(See PubMed article )
  Ms / 1:1,000
PA1-066 was used in immunohistochemistry and western blot to perform expression profiling during a 12-day time course of C2C12 myoblasts' differentiation

FASEB J. 2004 Feb;18(2):403-5.
"Expression profiling and identification of novel genes involved in myogenic differentiation."
Author(s): Tomczak KK, Marinescu VD, Ramoni MF, Sanoudou D, Montanaro F, Han M, Kunkel LM, Kohane IS, Beggs AH
Number of Citations: 1
(See PubMed article )
  Ms / 1:1000
PA1-066 was used in immunohistochemistry, immunoprecipitation, and western blot to investigate the mechanism for the involvement of caveolin-3 in the pathogenesis of muscle caveolinopathy

Int J Biochem Cell Biol. 2011 May;43(5):713-20.
"Caveolin-3 is a direct molecular partner of the Cav1.1 subunit of the skeletal muscle L-type calcium channel."
Author(s): Couchoux H, Bichraoui H, Chouabe C, Altafaj X, Bonvallet R, Allard B, Ronjat M, Berthier C
Number of Citations: 1
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Ms / 1:1,500
PA1-066 was used in immunocytochemistry to study the role of caveolin-3 in L-type calcium channel membrane function and localization in skeletal muscle cells .

J Physiol. 2007 May 1;580(Pt.3):745-54.
"Loss of caveolin-3 induced by the dystrophy-associated P104L mutation impairs L-type calcium channel function in mouse skeletal muscle cells."
Author(s): Couchoux H, Allard B, Legrand C, Jacquemond V, Berthier C
Number of Citations: 1
(See PubMed article )
  Rt / 1:100
PA1-066 was used in immunocytochemistry to study the function of cardiac sodium/potassium-ATPase

Am J Physiol Cell Physiol. 2003 Jun;284(6):C1550-60.
"Role of caveolae in signal-transducing function of cardiac Na+/K+-ATPase."
Author(s): Liu L, Mohammadi K, Aynafshar B, Wang H, Li D, Liu J, Ivanov AV, Xie Z, Askari A
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
PA1-066 was used in immunocytochemistry to examine the role of ryanodine receptors in the development of rat cardiomyocytes

J Mol Cell Cardiol. 2008 Jun;44(6):1032-44.
"Ca2+ sparks and cellular distribution of ryanodine receptors in developing cardiomyocytes from rat."
Author(s): Snopko RM, Ramos-Franco J, Di Maio A, Karko KL, Manley C, Piedras-Rentería E, Mejía-Alvarez R
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Ms / 1:500
PA1-066 was used in immunohistochemistry and western blot to perform expression profiling during a 12-day time course of C2C12 myoblasts' differentiation

FASEB J. 2004 Feb;18(2):403-5.
"Expression profiling and identification of novel genes involved in myogenic differentiation."
Author(s): Tomczak KK, Marinescu VD, Ramoni MF, Sanoudou D, Montanaro F, Han M, Kunkel LM, Kohane IS, Beggs AH
Number of Citations: 1
(See PubMed article )
  Ms / 0
PA1-066 was used in immunohistochemistry, immunoprecipitation, and western blot to investigate the mechanism for the involvement of caveolin-3 in the pathogenesis of muscle caveolinopathy

Int J Biochem Cell Biol. 2011 May;43(5):713-20.
"Caveolin-3 is a direct molecular partner of the Cav1.1 subunit of the skeletal muscle L-type calcium channel."
Author(s): Couchoux H, Bichraoui H, Chouabe C, Altafaj X, Bonvallet R, Allard B, Ronjat M, Berthier C
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Ms / 0
PA1-066 was used in immunohistochemistry, immunoprecipitation, and western blot to investigate the mechanism for the involvement of caveolin-3 in the pathogenesis of muscle caveolinopathy

Int J Biochem Cell Biol. 2011 May;43(5):713-20.
"Caveolin-3 is a direct molecular partner of the Cav1.1 subunit of the skeletal muscle L-type calcium channel."
Author(s): Couchoux H, Bichraoui H, Chouabe C, Altafaj X, Bonvallet R, Allard B, Ronjat M, Berthier C
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
PA1-066 was used in immunoprecipitation to investigate the interaction between GluR1 and SAP97.

J Neurosci. 2001 Oct 1;21(19):7506-16.
"Synapse-associated protein 97 selectively associates with a subset of AMPA receptors early in their biosynthetic pathway."
Author(s): Sans N, Racca C, Petralia RS, Wang YX, McCallum J, Wenthold RJ
Number of Citations: 20
(See PubMed article )
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