Part of Thermo Fisher Scientific

Cannabinoid Receptor I Polyclonal Antibody

Cannabinoid Receptor I Antibody for FACS, ICC, IHC (P), Western blot

Synonyms:
CB1, Cnr1
Entrez Gene ID:
(Human) 1268
UniProt ID:
P21554
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Species Reactivity: Human (Hu) Murine (Ms) Rat (Rt) Non-human primate (Nhp) Chicken (Ck) Amphibian (Am) Fish (Fs)
Immunogen: A fusion protein containing the first 77 amino acid residues from rat CB1.
Ordering Information
Pierce Cannabinoid Receptor I Polyclonal Antibody
Product Number Pkg. Size Price Purchase
PA1-745 100 µl $354.00
Add Cannabinoid Receptor I Polyclonal Antibody to your cart.


Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 µl of affinity purified IgG (3.3 mg/ml) in PBS containing 50% glycerol, 1 mg/ml BSA and 0.05% sodium azide.


Applications Dilution *
Flow Cytometry (FACS) Assay dependent
Western Blot (WB) Assay dependent
Immunocytochemistry (ICC) 1:1,000
Immunohistochemistry (Paraffin) (IHC (P)) Assay dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA1-745 detects cannabinoid receptor 1 (CB1) from human, rat, monkey, mouse, chicken, salamander and goldfish tissues.

PA1-745 has been successfully used in Western blot, FACS, immunohistochemistry, and immunocytochemistry procedures. By Western blot, this antibody detects a ~60 kDa protein representing CB1 and less intense bands of ~23 kDa, ~72 kDa, and ~180 kDa from rat brain homogenate. Immunocytochemical staining of CB1 in living or fixed cultured hippocampal neurons with PA1-745 results in intense, punctate staining along processes, with little staining over cell bodies.

PA1-745 antigen is a fusion protein containing the first 77 amino acid residues of the rat CB1 receptor.

Figure 1 shows immunolocalization of cannibinoid receptor 1 in rat hippocampus using PA1-745.

Figure 2 shows staining of the cannibinoid receptor 1 in transverse spinal cord sections of E6 chick embryo using PA1-745.
General Information
Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P/Q type calcium channels.

CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.
Product Images
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  • IF using PA1-745
    Immunofluorescence with anti-Cannabinoid Receptor I Polyclonal Antibody (PA1-745)

    Immunofluorescence with anti-Cannabinoid Receptor I Polyclonal Antibody (PA1-745)

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  • IHC using PA1-745
    Immunohistochemistry with anti-Cannabinoid Receptor I Polyclonal Antibody (PA1-745)

    Immunohistochemistry with anti-Cannabinoid Receptor I Polyclonal Antibody (PA1-745)

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PubMed References:
Flow Cytometry
  Species / Dilution Summary
  Rt / Not Cited
 PA1-745 was used in western blot and flow cytometry cytometry to investigate the function of cannabinoid receptors in the anti-inflammatory effects of CB1 ligands in mast cells.
Biochem J. 2005 Jun 1;388(Pt 2):465-73.
"Anti-inflammatory potential of CB1-mediated cAMP elevation in mast cells."
Author(s): Small-Howard AL, Shimoda LM, Adra CN, Turner H
Number of Citations: 1
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / Not Cited
 PA1-745 was used in immunocytochemistry, immunoprecipitation and western blot to study the interactions between striatal adenosine A2A and cannabinoid CB1 receptors
Neuropsychopharmacology. 2007 Nov;32(11):2249-59.
"Striatal adenosine A2A and cannabinoid CB1 receptors form functional heteromeric complexes that mediate the motor effects of cannabinoids."
Author(s): Carriba P, Ortiz O, Patkar K, Justinova Z, Stroik J, Themann A, Müller C, Woods AS, Hope BT, Ciruela F, Casadó V, Canela EI, Lluis C, Goldberg SR, Moratalla R, Franco R, Ferré S
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Ft / 1:500
 PA1-745 was used in immunohistochemistry to investigate the antiemetic effect of THC in the ferret
Am J Physiol Gastrointest Liver Physiol. 2003 Sep;285(3):G566-76.
"Delta9-tetrahydrocannabinol selectively acts on CB1 receptors in specific regions of dorsal vagal complex to inhibit emesis in ferrets."
Author(s): Van Sickle MD, Oland LD, Mackie K, Davison JS, Sharkey KA
Number of Citations: 1
(See PubMed article )
  Hu / 1:100
 PA1-745 was used in immunohistochemistry and western blot to study the expression of the endocannabinoid system's various components and the enzymes related to cannabinoid degradation and biosynthesis in normal human colonic tissue in comparison with
PLoS One. 2009 Sep 4;4(9):e6893.
"Ulcerative colitis induces changes on the expression of the endocannabinoid system in the human colonic tissue."
Author(s): Marquéz L, Suárez J, Iglesias M, Bermudez-Silva FJ, Rodríguez de Fonseca F, Andreu M
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 PA1-745 was used in immunohistochemistry to characterize sn1-DAG lipases.
J Cell Biol. 2003 Nov 10;163(3):463-8.
"Cloning of the first sn1-DAG lipases points to the spatial and temporal regulation of endocannabinoid signaling in the brain."
Author(s): Bisogno T, Howell F, Williams G, Minassi A, Cascio MG, Ligresti A, Matias I, Schiano-Moriello A, Paul P, Williams EJ, Gangadharan U, Hobbs C, Di Marzo V, Doherty P
Number of Citations: 1
(See PubMed article )
  Ms / 1:100
 PA1-745 was used in immunohistochemistry to investigate the mechanism for the colonic propulsion mediated by cannabinoid-1 receptors
Am J Physiol Gastrointest Liver Physiol. 2009 Jan;296(1):G119-28.
"Cannabinoid-1 (CB1) receptors regulate colonic propulsion by acting at motor neurons within the ascending motor pathways in mouse colon."
Author(s): Sibaev A, Yüce B, Kemmer M, Van Nassauw L, Broedl U, Allescher HD, Göke B, Timmermans JP, Storr M
Number of Citations: 1
(See PubMed article )
  Rt / 1:2,000
 PA1-745 was used in immunohistochemistry and western blot to investigate the distribution of cannabinoid CB1 receptors in the adult rat brain
Neuroscience. 1998 Mar;83(2):393-411.
"Immunohistochemical distribution of cannabinoid CB1 receptors in the rat central nervous system."
Author(s): Tsou K, Brown S, Sañudo-Peña MC, Mackie K, Walker JM
Number of Citations: 104
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Rt / Not Cited
 PA1-745 was used in immunocytochemistry, immunoprecipitation and western blot to study the interactions between striatal adenosine A2A and cannabinoid CB1 receptors
Neuropsychopharmacology. 2007 Nov;32(11):2249-59.
"Striatal adenosine A2A and cannabinoid CB1 receptors form functional heteromeric complexes that mediate the motor effects of cannabinoids."
Author(s): Carriba P, Ortiz O, Patkar K, Justinova Z, Stroik J, Themann A, Müller C, Woods AS, Hope BT, Ciruela F, Casadó V, Canela EI, Lluis C, Goldberg SR, Moratalla R, Franco R, Ferré S
Number of Citations: 1
(See PubMed article )
Western Blot
  Species / Dilution Summary
  Hu / Not Cited
 PA1-745 was used in western blot and flow cytometry cytometry to investigate the function of cannabinoid receptors in the anti-inflammatory effects of CB1 ligands in mast cells.
Biochem J. 2005 Jun 1;388(Pt 2):465-73.
"Anti-inflammatory potential of CB1-mediated cAMP elevation in mast cells."
Author(s): Small-Howard AL, Shimoda LM, Adra CN, Turner H
Number of Citations: 1
(See PubMed article )
  Hu / 1:250
 PA1-745 was used in immunohistochemistry and western blot to study the expression of the endocannabinoid system's various components and the enzymes related to cannabinoid degradation and biosynthesis in normal human colonic tissue in comparison with
PLoS One. 2009 Sep 4;4(9):e6893.
"Ulcerative colitis induces changes on the expression of the endocannabinoid system in the human colonic tissue."
Author(s): Marquéz L, Suárez J, Iglesias M, Bermudez-Silva FJ, Rodríguez de Fonseca F, Andreu M
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 PA1-745 was used in western blot and flow cytometry cytometry to investigate the function of cannabinoid receptors in the anti-inflammatory effects of CB1 ligands in mast cells.
Biochem J. 2005 Jun 1;388(Pt 2):465-73.
"Anti-inflammatory potential of CB1-mediated cAMP elevation in mast cells."
Author(s): Small-Howard AL, Shimoda LM, Adra CN, Turner H
Number of Citations: 1
(See PubMed article )
  Ms / 1:250
 PA1-745 was used in western blot to evaluate the neuroprotective and neuroproliferative effects of FGF-2 in a transgenic mouse model of Huntingdon disease
Proc Natl Acad Sci U S A. 2005 Dec 13;102(50):18189-94.
"FGF-2 promotes neurogenesis and neuroprotection and prolongs survival in a transgenic mouse model of Huntington's disease."
Author(s): Jin K, LaFevre-Bernt M, Sun Y, Chen S, Gafni J, Crippen D, Logvinova A, Ross CA, Greenberg DA, Ellerby LM
Number of Citations: 1
(See PubMed article )
  Rt / 1:500
 PA1-745 was used in immunohistochemistry and western blot to investigate the distribution of cannabinoid CB1 receptors in the adult rat brain
Neuroscience. 1998 Mar;83(2):393-411.
"Immunohistochemical distribution of cannabinoid CB1 receptors in the rat central nervous system."
Author(s): Tsou K, Brown S, Sañudo-Peña MC, Mackie K, Walker JM
Number of Citations: 104
(See PubMed article )
  Rt / Not Cited
 PA1-745 was used in western blot and flow cytometry cytometry to investigate the function of cannabinoid receptors in the anti-inflammatory effects of CB1 ligands in mast cells.
Biochem J. 2005 Jun 1;388(Pt 2):465-73.
"Anti-inflammatory potential of CB1-mediated cAMP elevation in mast cells."
Author(s): Small-Howard AL, Shimoda LM, Adra CN, Turner H
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
 PA1-745 was used in immunocytochemistry, immunoprecipitation and western blot to study the interactions between striatal adenosine A2A and cannabinoid CB1 receptors
Neuropsychopharmacology. 2007 Nov;32(11):2249-59.
"Striatal adenosine A2A and cannabinoid CB1 receptors form functional heteromeric complexes that mediate the motor effects of cannabinoids."
Author(s): Carriba P, Ortiz O, Patkar K, Justinova Z, Stroik J, Themann A, Müller C, Woods AS, Hope BT, Ciruela F, Casadó V, Canela EI, Lluis C, Goldberg SR, Moratalla R, Franco R, Ferré S
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)