Calreticulin Antibody
Calreticulin Polyclonal Antibody for ICC, IF, IP, Western blot
Synonyms:
Calregulin, CRP55, CaBP3, Calsequestrin-Like Protein, Ro/SS-A Antigen
Entrez Gene ID:
UniProt ID:
(Hu) P27797, (Ms) P14211, (Rt) P18418
| Details | |
| Host / Isotype: | Chicken |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) Mouse (Ms) Rat (Rt) Canine (Ca) Rabbit (Rb) Hamster (Hm) |
| Immunogen: | Synthetic Peptide: D(399) E K E E D E E E S P G Q A K D(414). |
| Ordering Information | ||||
| Pierce Calreticulin Antibody |
| Storage: | -20° C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µl of epitope affinity purified IgY (1 mg/ml) containing 1 mg/ml BSA and 0.05% sodium azide. |
| Applications | Dilution * |
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC) | Assay dependent |
| Immunofluorescence (IF) | Assay dependent |
| Immunoprecipitation (IP) | Assay dependent |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA1-903 detects calreticulin from human, rat, mouse, hamster, rabbit and canine tissues. PA1-903 has been successfully used in Western blot, immunoprecipitation, immunocytochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~60 kDa protein representing calreticulin and an ~120 kDa unidentified protein from rat brain extract. PA1-903 immunizing peptide corresponds to amino acid residues 399-414 from mouse calreticulin. PA1-903 immunizing peptide (Cat. # PEP-017) is available for use in neutralization and control experiments. |
| General Information |
| Calreticulin is the major calcium binding protein found in smooth muscle sarcoplasmic reticulum (SR) and non-muscle endoplasmic reticulum (ER) membranes. This protein was originally identified in SR membranes and plays a minor role in calcium storage in skeletal and cardiac muscle SR. Calreticulin is also known as calregulin, CRP55, CaBP3, calsequestrin-like protein and Ro/SS-A antigen. Calreticulin binds calcium with low affinity and high capacity, however it also exhibits a single high affinity binding site. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of calreticulin and other resident ER proteins including glucose regulated protein 78 (GRP78), GRP94 and protein disulfide isomerase (PDI). This sequence is responsible for the retention of newly synthesized proteins within the ER lumen. This retention is reported to be mediated by a KDEL receptor. Recent reports indicate that calreticulin can act as a modulator of the regulation of gene transcription by nuclear hormone receptors and may also act as a molecular chaperone. |
| Product Images |
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Calreticulin Antibody (PA1-903) in WB
Western Blot with anti-Calreticulin Polyclonal Antibody (PA1-903)
Figure 1 shows a Western blot of calreticulin on HeLa cell extract using PA1-903. -
Calreticulin Antibody (PA1-903) in WB
Western Blot with anti-Calreticulin Polyclonal Antibody (PA1-903)
Figure 2 shows a Western blot of calreticulin (transfected and endogenous levels) on HEK cell microsomes using PA1-903.
| PubMed References: |
| Immunocytochemistry | ||
| Species / Dilution | Summary | |
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Am / 1:30 |
PA1-903 was used in immunocytochemistry to investigate the role of CRT in IP3-mediated caclium signaling
Cell. 1995 Sep 8;82(5):765-71.
"Calreticulin inhibits repetitive intracellular Ca2+ waves." Author(s): Camacho P, Lechleiter JD Number of Citations: 43 (See PubMed article  )
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Hu / Not Cited |
PA1-903 was used in immunocytochemistry to study the mechanisms of macropinocytosis and apoptotic cell removal
J Exp Med. 2001 Sep 17;194(6):781-95.
"C1q and mannose binding lectin engagement of cell surface calreticulin and CD91 initiates macropinocytosis and uptake of apoptotic cells." Author(s): Ogden CA, deCathelineau A, Hoffmann PR, Bratton D, Ghebrehiwet B, Fadok VA, Henson PM Number of Citations: 82 (See PubMed article )
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| Immunoprecipitation | ||
| Species / Dilution | Summary | |
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Hu / Not Cited |
PA1-903 was used in immunoprecipitation and western blot to investigate the role of divalent metal transporter-1 in conveyance of iron from paraferritin to ferrochelatase
Am J Physiol Gastrointest Liver Physiol. 2002 Mar;282(3):G534-9.
"The ferrireductase paraferritin contains divalent metal transporter as well as mobilferrin." Author(s): Umbreit JN, Conrad ME, Hainsworth LN, Simovich M Number of Citations: 1 (See PubMed article )
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Nhp / Not Cited |
PA1-903 was used in immunoprecipitation and western blot to investigate the interaction of calreticulin with human immunodeficiency virus type 1 envelope glycoprotein
J Biol Chem. 1996 Jan 5;271(1):97-103.
"Calreticulin interacts with newly synthesized human immunodeficiency virus type 1 envelope glycoprotein, suggesting a chaperone function similar to that of calnexin." Author(s): Otteken A, Moss B Number of Citations: 28 (See PubMed article )
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| Western Blot | ||
| Species / Dilution | Summary | |
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Hu / Not Cited |
PA1-903 was used in western blot to demonstrate that calreticulin in ER is another novel CAS-sensitive chaperone
J Biol Chem. 1995 Sep 1;270(35):20298-304.
"Chaperone function of calreticulin when expressed in the endoplasmic reticulum as the membrane-anchored and soluble forms." Author(s): Wada I, Imai S, Kai M, Sakane F, Kanoh H Number of Citations: 21 (See PubMed article )
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Hu / Not Cited |
PA1-903 was used in western blot to investigate how HLA-B27 interact with the peptide loading complex
Int Immunol. 2001 Oct;13(10):1275-82.
"Interactions of HLA-B27 with the peptide loading complex as revealed by heavy chain mutations." Author(s): Harris MR, Lybarger L, Myers NB, Hilbert C, Solheim JC, Hansen TH, Yu YY Number of Citations: 10 (See PubMed article )
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Hu / 1:800 |
PA1-903 was used in immunoprecipitation and western blot to investigate the role of divalent metal transporter-1 in conveyance of iron from paraferritin to ferrochelatase
Am J Physiol Gastrointest Liver Physiol. 2002 Mar;282(3):G534-9.
"The ferrireductase paraferritin contains divalent metal transporter as well as mobilferrin." Author(s): Umbreit JN, Conrad ME, Hainsworth LN, Simovich M Number of Citations: 1 (See PubMed article )
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Hu / 1:800 |
PA1-903 was used in western blot to study the intestinal iron uptake and the involvement of mucin
Am J Hematol. 2003 Sep;74(1):32-45.
"Localization of the iron transport proteins Mobilferrin and DMT-1 in the duodenum: the surprising role of mucin." Author(s): Simovich M, Hainsworth LN, Fields PA, Umbreit JN, Conrad ME Number of Citations: 1 (See PubMed article )
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Hu / 0 |
PA1-903 was used in western blot to study the relationship between Ins(1,4,5)P3 receptor-mediated Ca2+ signaling and the induction of autophagy
Autophagy. 2011 Dec;7(12):1472-89.
"Ins(1,4,5)P3 receptor-mediated Ca2+ signaling and autophagy induction are interrelated." Author(s): Decuypere JP, Welkenhuyzen K, Luyten T, Ponsaerts R, Dewaele M, Molgó J, Agostinis P, Missiaen L, De Smedt H, Parys JB, Bultynck G Number of Citations: 2 (See PubMed article )
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Ms / Not Cited |
PA1-903 was used in western blot to study the interaction between ERp57 and mouse MHC class I molecules.
J Immunol. 2001 Jun 1;166(11):6686-92.
"Association of ERp57 with mouse MHC class I molecules is tapasin dependent and mimics that of calreticulin and not calnexin." Author(s): Harris MR, Lybarger L, Yu YY, Myers NB, Hansen TH Number of Citations: 6 (See PubMed article )
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Ms / Not Cited |
PA1-903 was used in western blot to perform the global proteomics surveys of cardiac ventricle of a phospholamban mutant mouse
Mol Cell Proteomics. 2008 Mar;7(3):519-33.
"Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses." Author(s): Gramolini AO, Kislinger T, Alikhani-Koopaei R, Fong V, Thompson NJ, Isserlin R, Sharma P, Oudit GY, Trivieri MG, Fagan A, Kannan A, Higgins DG, Huedig H, Hess G, Arab S, Seidman JG, Seidman CE, Frey B, Perry M, Backx PH, Liu PP, MacLennan DH, Emili A Number of Citations: 1 (See PubMed article )
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Ms / 0 |
PA1-903 was used in western blot to study the relationship between Ins(1,4,5)P3 receptor-mediated Ca2+ signaling and the induction of autophagy
Autophagy. 2011 Dec;7(12):1472-89.
"Ins(1,4,5)P3 receptor-mediated Ca2+ signaling and autophagy induction are interrelated." Author(s): Decuypere JP, Welkenhuyzen K, Luyten T, Ponsaerts R, Dewaele M, Molgó J, Agostinis P, Missiaen L, De Smedt H, Parys JB, Bultynck G Number of Citations: 2 (See PubMed article )
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Nhp / 1:1,000 |
PA1-903 was used in immunoprecipitation and western blot to investigate the interaction of calreticulin with human immunodeficiency virus type 1 envelope glycoprotein
J Biol Chem. 1996 Jan 5;271(1):97-103.
"Calreticulin interacts with newly synthesized human immunodeficiency virus type 1 envelope glycoprotein, suggesting a chaperone function similar to that of calnexin." Author(s): Otteken A, Moss B Number of Citations: 28 (See PubMed article )
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Rt / 1:500 |
PA1-903 was used in western blot to investigate the role of cardiac mitochondrial connexin 43 in apoptosis
Biochem Biophys Res Commun. 2007 Jan 5;352(1):97-103.
"Cardiac mitochondrial connexin 43 regulates apoptosis." Author(s): Goubaeva F, Mikami M, Giardina S, Ding B, Abe J, Yang J Number of Citations: 6 (See PubMed article )
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(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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Calreticulin Antibodies > Calreticulin Polyclonal Antibody PA1-903
