Calcium Sensing Receptor Antibody (5C10, ADD)

Calcium Sensing Receptor Monoclonal Antibody for Western blot, IF, IHC (F), ELISA

>> See 8 other antibodies for CASR (CaSR, Parathyroid Cell calcium-sensing receptor)
Synonyms:
CaSR, Parathyroid Cell calcium-sensing receptor
Details
Host / Isotype: Mouse / IgG2a
Class: Monoclonal
Type: Antibody
Clone: 5C10, ADD
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Bovine (Bv)
Published Species Reactivity: Bovine (Bv), Human (Hu), Mouse (Ms), Porcine (Po), Rat (Rt)
Immunogen: Synthetic peptide corresponding to residues (214) A D D D Y G R P G I E K F RE E A E E R D I (235) of human calcium sensing receptor.
Ordering Information
Pierce Calcium Sensing Receptor Antibody (5C10, ADD)
Product #
MA1-934
Size
100 µg
Price
$360.00
Purchase
Add Calcium Sensing Receptor Antibody (5C10, ADD) to your cart.
Tested Applications Dilution *
Western Blot (WB) 2 ug/ml
Immunofluorescence (IF) Assay dependent
Immunohistochemistry (Frozen) (IHC (F)) 2 ug/ml
ELISA (ELISA) Assay dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Flow Cytometry (FACS) See publications below
Immunoprecipitation (IP) See publications below
ELISA (ELISA) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Protein A
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA1-934 detects human, bovine, mouse, and rat calcium sensing receptor.

MA1-934 has been successfully used in Western blot, immunohistochemistry, and ELISA procedures. By Western blot, this antibody detects an ~ 150 and 130 kDa proteins from human HEK-293 cells.This antibody has also been used in immunohistochemical on frozen bovine parathyroid gland and rat thyroid gland.

The MA1-934 immunogen is a synthetic peptide corresponding to residues (214) A D D D Y G R P G I E K F RE E A E E R D I (235) of human calcium sensing receptor.
General Information
The calcium sensing receptor (CaSR) has been shown to play a major role in regulating parathyroid hormone secretion and subsequently influencing the calcium concentration of extracellular fluids. Based on sequence analysis of cDNA clones it appears that the CaSR is similar to the 7-transmembrane-domain G-protein-coupled receptor superfamily. Changes in extracellular calcium are thought to modulate a balance between proliferation and differentiation in a variety of cell types. In normal primary keratinocytes and breast epithelial cells, proliferation is inhibited and elevated extracellular calcium levels trigger differentiation. Malignant transformations of these cell types are accompanied by a loss of responsiveness to the anti-proliferative effects of elevated extracellular calcium. Several disorders of calcium homeostasis have been linked to mutations in the CaSR. These include familial hypocalciuric hypercalcemia (FHH), neonatal severe hyperparathyroidism (NSHPT), and autosomal dominant hypocalemia (ADHypo).
Product Images
  • Calcium Sensing Receptor Antibody (MA1-934) in WB
    MA1-934_1_07232010.jpg

    Western Blot with anti-Calcium Sensing Receptor Monoclonal Antibody [5C10, ADD] (MA1-934) (MA1-934_1_07232010.jpg)

    Western Blot with anti-Calcium Sensing Receptor Monoclonal Antibody [5C10, ADD] (MA1-934)

    Western blot analysis of calcium sensing receptor in HEK7-2 cell extract using Calcium Sensing Receptor Monoclonal Antibody (Product # MA1-934).
  • Calcium Sensing Receptor Antibody (MA1-934) in IF
    CASR-Antibody-MA1-934-Immunofluorescence-IF.jpg

    Immunofluorescence with anti-Calcium Sensing Receptor Monoclonal Antibody [5C10, ADD] (MA1-934) (CASR-Antibody-MA1-934-Immunofluorescence-IF.jpg)

    Immunofluorescence with anti-Calcium Sensing Receptor Monoclonal Antibody [5C10, ADD] (MA1-934)

    Immunofluorescent analysis of bovine corneal epithelium (CE) limbus tissue extracts using Calcium Sensing Receptor Monoclonal Antibody (Product # MA1-934).
  • Calcium Sensing Receptor Antibody (MA1-934) in IHC
    MA1934_cropped_image_for_website.jpg

    Immunohistochemistry with anti-Calcium Sensing Receptor Monoclonal Antibody [5C10, ADD] (MA1-934) (MA1934_cropped_image_for_website.jpg)

    Immunohistochemistry with anti-Calcium Sensing Receptor Monoclonal Antibody [5C10, ADD] (MA1-934)

    Immunohistochemical analysis of mouse stomach stained with Calcium Sensing Receptor Monoclonal Antibody (Product # MA1-934). Fresh stomach tissue was fixed in 4% formalin for 1 hour and then incubated overnight at 4ºC in 25% sucrose before embedding in tissue freezing medium. Antigen retrieval was carried out on 8µm cryo-sections by incubating in sodium citrate buffer for 45 minutes at 4ºC, immersing in sodium citrate buffer for 10 minutes at 100ºC before washing 3 times for 5 minutes each in 1X PBS. Sections were then blocked in blocking buffer (0.3% Triton X-100 in 1X PBS containing 10% normal goat serum) for 30 minutes at RT before staining with MA1-934 (diluted 1:100 in blocking buffer) overnight at 4ºC followed by a fluorophore-conjugated goat anti-mouse IgG secondary antibody for 2 hours at RT. Sections were also stained with DAPI nuclear stain (blue). MA1-934 positive cells (green) were found at the base of the antral glands in the mouse stomach. Data courtesy of the Innovators Program.
  • Calcium Sensing Receptor Antibody (MA1-934) in IHC
    MA1-934_IHC_Brain_Tissue.jpg

    Immunohistochemistry with anti-Calcium Sensing Receptor Monoclonal Antibody [5C10, ADD] (MA1-934) (MA1-934_IHC_Brain_Tissue.jpg)

    Immunohistochemistry with anti-Calcium Sensing Receptor Monoclonal Antibody [5C10, ADD] (MA1-934)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Calcium Sensing Receptor (MA1-934) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Calcium Sensing Receptor Antibody (MA1-934) in IHC
    MA1-934_IHC_kidney_Tissue.jpg

    Immunohistochemistry with anti-Calcium Sensing Receptor Monoclonal Antibody [5C10, ADD] (MA1-934) (MA1-934_IHC_kidney_Tissue.jpg)

    Immunohistochemistry with anti-Calcium Sensing Receptor Monoclonal Antibody [5C10, ADD] (MA1-934)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Calcium Sensing Receptor (MA1-934) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Bv / Not Cited
MA1-934 was used in immunohistochemistry and western blot to examine the expression of the calcium-sensing receptor (CaR) in vascular smooth muscle cells

Cardiovasc Res. 2009 Feb 1;81(2):260-8.
"Calcification is associated with loss of functional calcium-sensing receptor in vascular smooth muscle cells."
Author(s): Alam MU, Kirton JP, Wilkinson FL, Towers E, Sinha S, Rouhi M, Vizard TN, Sage AP, Martin D, Ward DT, Alexander MY, Riccardi D, Canfield AE
Number of Citations: 1
(See PubMed article )
  Hu / 40 ug/ml
MA1-934 was used in ELISA, flow cytometry and western blot to produce and characterize novel anti-human calcium-sensing receptor monoclonal antibodies

J Bone Miner Res. 1997 Nov;12(11):1780-8.
"Monoclonal antibodies against synthetic peptides corresponding to the extracellular domain of the human Ca2+ receptor: characterization and use in studying concanavalin A inhibition."
Author(s): Goldsmith PK, Fan G, Miller JL, Rogers KV, Spiegel AM
Number of Citations: 1
(See PubMed article )
  Hu / 2 ug/ml
MA1-934 was used in western blot to purify the extracellular domain of the human calcium receptor and perform corresponding biochemical analysis

J Biol Chem. 1999 Apr 16;274(16):11303-9.
"Expression, purification, and biochemical characterization of the amino-terminal extracellular domain of the human calcium receptor."
Author(s): Goldsmith PK, Fan GF, Ray K, Shiloach J, McPhie P, Rogers KV, Spiegel AM
Number of Citations: 3
(See PubMed article )
  Hu / Not Cited
MA1-934 was used in immunohistochemistry and western blot to examine the expression of the calcium-sensing receptor (CaR) in vascular smooth muscle cells

Cardiovasc Res. 2009 Feb 1;81(2):260-8.
"Calcification is associated with loss of functional calcium-sensing receptor in vascular smooth muscle cells."
Author(s): Alam MU, Kirton JP, Wilkinson FL, Towers E, Sinha S, Rouhi M, Vizard TN, Sage AP, Martin D, Ward DT, Alexander MY, Riccardi D, Canfield AE
Number of Citations: 1
(See PubMed article )
  Hu / 1:1000
MA1-934 was used in immunocytochemistry and western blot to investigate the influence of nickel on calcium signalling and pathophysiological responses

Chem Biol Interact. 2010 Jan 5;183(1):25-33.
"Nickel induces intracellular calcium mobilization and pathophysiological responses in human cultured airway epithelial cells."
Author(s): Cortijo J, Milara J, Mata M, Donet E, Gavara N, Peel SE, Hall IP, Morcillo EJ
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA1-934 was used in western blot to identify how phosphorylation status of calcium-sensing receptors affects parathyroid function

J Biol Chem. 2010 May 7;285(19):14170-7.
"Increased receptor stimulation elicits differential calcium-sensing receptor(T888) dephosphorylation."
Author(s): McCormick WD, Atkinson-Dell R, Campion KL, Mun HC, Conigrave AD, Ward DT
Number of Citations: 2
(See PubMed article )
  Hu / 1:1000
MA1-934 was used in immunocytochemistry, immunohistochemistry, and western blot to investigate the effect of calcium-sensing receptor on airway epithelial repair

Biochem Pharmacol. 2010 Jul 15;80(2):236-46.
"Extracellular calcium-sensing receptor mediates human bronchial epithelial wound repair."
Author(s): Milara J, Mata M, Serrano A, Peiró T, Morcillo EJ, Cortijo J
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA1-934 was used in western blot to investigate the association between 990G allele and sensitivity of calcium-sensing receptor towards calcimimetic R-568

J Mol Endocrinol. 2010 Oct;45(4):245-56.
"Calcimimetic R-568 effects on activity of R990G polymorphism of calcium-sensing receptor."
Author(s): Terranegra A, Ferraretto A, Dogliotti E, Scarpellini M, Corbetta S, Barbieri AM, Spada A, Arcidiacono T, Rainone F, Aloia A, Cusi D, Vezzoli G, Soldati L
Number of Citations: 0
(See PubMed article )
  Hu / Not Cited
MA1-934 was used in western blot to investigate the mediation of the cell surface trafficking of hCaR by Rab1

Endocrinology. 2010 Nov;151(11):5114-23.
"Rab1 small GTP-binding protein regulates cell surface trafficking of the human calcium-sensing receptor."
Author(s): Zhuang X, Adipietro KA, Datta S, Northup JK, Ray K
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA1-934 was used in western blot to identify a calcium-sensing receptor mutation in autosomal dominant hypocalcemia

Eur J Endocrinol. 2011 Mar;164(3):429-35.
"A novel mutation of the primary protein kinase C phosphorylation site in the calcium-sensing receptor causes autosomal dominant hypocalcemia."
Author(s): Lazarus S, Pretorius CJ, Khafagi F, Campion KL, Brennan SC, Conigrave AD, Brown EM, Ward DT
Number of Citations: 1
(See PubMed article )
  Hu / 1:2000
MA1-934 was used in immunocytochemistry and western blot to study the effect of calcimimetic R-568 on nitric oxide release in human endothelial cells

PLoS One. 2012;7(1):e30682.
"Calcimimetic R-568 and its enantiomer S-568 increase nitric oxide release in human endothelial cells."
Author(s): Bonomini M, Giardinelli A, Morabito C, Di Silvestre S, Di Cesare M, Di Pietro N, Sirolli V, Formoso G, Amoroso L, Mariggiò MA, Pandolfi A
Number of Citations: 1
(See PubMed article )
  Hu / 1:200
MA1-934 was used in immunohistochemistry and western blot to study role of enhanced Ca(2+)-sensing receptor function in idiopathic pulmonary arterial hypertension

Circ Res. 2012 Aug 3;111(4):469-81.
"Enhanced Ca(2+)-sensing receptor function in idiopathic pulmonary arterial hypertension."
Author(s): Yamamura A, Guo Q, Yamamura H, Zimnicka AM, Pohl NM, Smith KA, Fernandez RA, Zeifman A, Makino A, Dong H, Yuan JX
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA1-934 was used in western blot to investigate the involvement of regulator of G protein signaling 5 RGS5 in parathyroid tumorigenesis

Mol Endocrinol. 2011 May;25(5):867-76.
"Regulator of G protein signaling 5 is highly expressed in parathyroid tumors and inhibits signaling by the calcium-sensing receptor."
Author(s): Koh J, Dar M, Untch BR, Dixit D, Shi Y, Yang Z, Adam MA, Dressman H, Wang X, Gesty-Palmer D, Marks JR, Spurney R, Druey KM, Olson JA Jr
Number of Citations: 0
(See PubMed article )
  Po / 0.2 ug/ml
MA1-934 was used in western blot and immunohistochemistry to investigate the possible regulation of vasodilator actions by CaSR

Circ Res. 2005 Aug 19;97(4):391-8.
"Evidence in favor of a calcium-sensing receptor in arterial endothelial cells: studies with calindol and Calhex 231."
Author(s): Weston AH, Absi M, Ward DT, Ohanian J, Dodd RH, Dauban P, Petrel C, Ruat M, Edwards G
Number of Citations: 1
(See PubMed article )
  Rt / 40 ug/ml
MA1-934 was used in ELISA, flow cytometry and western blot to produce and characterize novel anti-human calcium-sensing receptor monoclonal antibodies

J Bone Miner Res. 1997 Nov;12(11):1780-8.
"Monoclonal antibodies against synthetic peptides corresponding to the extracellular domain of the human Ca2+ receptor: characterization and use in studying concanavalin A inhibition."
Author(s): Goldsmith PK, Fan G, Miller JL, Rogers KV, Spiegel AM
Number of Citations: 1
(See PubMed article )
  Rt / 1:500
MA1-934 was used in western blot to investigate whether TR signaling and gene expression of MHC and SR calcium-ATPase in the aged heart are affected by exercise training

Am J Physiol Heart Circ Physiol. 2004 May;286(5):H1696-705.
"Exercise training improves cardiac function-related gene levels through thyroid hormone receptor signaling in aged rats."
Author(s): Iemitsu M, Miyauchi T, Maeda S, Tanabe T, Takanashi M, Matsuda M, Yamaguchi I
Number of Citations: 1
(See PubMed article )
  Rt / 0.2 ug/ml
MA1-934 was used in western blot and immunohistochemistry to investigate the possible regulation of vasodilator actions by CaSR

Circ Res. 2005 Aug 19;97(4):391-8.
"Evidence in favor of a calcium-sensing receptor in arterial endothelial cells: studies with calindol and Calhex 231."
Author(s): Weston AH, Absi M, Ward DT, Ohanian J, Dodd RH, Dauban P, Petrel C, Ruat M, Edwards G
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
MA1-934 was used in western blot to study the effect of tripeptide glutathione on family C G-protein-coupled receptors.

J Biol Chem. 2006 Mar 31;281(13):8864-70.
"Activation of family C G-protein-coupled receptors by the tripeptide glutathione."
Author(s): Wang M, Yao Y, Kuang D, Hampson DR
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
MA1-934 was used in western blot to investigate the properties of the rat dorsal root ganglion calcium-sensing receptor and its important roles in calcium signaling

Am J Physiol Cell Physiol. 2007 May;292(5):C1895-905.
"Ca(2+) mobilization through dorsal root ganglion Ca(2+)-sensing receptor stably expressed in HEK293 cells."
Author(s): Awumey EM, Howlett AC, Putney JW Jr, Diz DI, Bukoski RD
Number of Citations: 1
(See PubMed article )
  Rt / 0
MA1-934 was used in immunocytochemistry, immunohistochemistry, and western blot to investigate the role of calcium in dentin formation and its mechanism

Calcif Tissue Int. 2008 Jan;82(1):57-65.
"Identification of a Ca2+-sensing receptor in rat trigeminal ganglia, sensory axons, and tooth dental pulp."
Author(s): Heyeraas KJ, Haug SR, Bukoski RD, Awumey EM
Number of Citations: 1
(See PubMed article )
  Rt / 1:200
MA1-934 was used in immunohistochemistry and western blot to study role of enhanced Ca(2+)-sensing receptor function in idiopathic pulmonary arterial hypertension

Circ Res. 2012 Aug 3;111(4):469-81.
"Enhanced Ca(2+)-sensing receptor function in idiopathic pulmonary arterial hypertension."
Author(s): Yamamura A, Guo Q, Yamamura H, Zimnicka AM, Pohl NM, Smith KA, Fernandez RA, Zeifman A, Makino A, Dong H, Yuan JX
Number of Citations: 1
(See PubMed article )
  Rt / 1:700 or 1:1000
MA1-934 was used in immunoprecipitation and western blot to study the role of extra-parathyroid calcium-sensing receptors in regulating blood calcium concentrations independently of PTH

J Clin Invest. 2012 Sep 4;122(9):3355-67.
"PTH-independent regulation of blood calcium concentration by the calcium-sensing receptor."
Author(s): Loupy A, Ramakrishnan SK, Wootla B, Chambrey R, de la Faille R, Bourgeois S, Bruneval P, Mandet C, Christensen EI, Faure H, Cheval L, Laghmani K, Collet C, Eladari D, Dodd RH, Ruat M, Houillier P
Number of Citations: 4
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / 1:1000
MA1-934 was used in immunocytochemistry and western blot to investigate the influence of nickel on calcium signalling and pathophysiological responses

Chem Biol Interact. 2010 Jan 5;183(1):25-33.
"Nickel induces intracellular calcium mobilization and pathophysiological responses in human cultured airway epithelial cells."
Author(s): Cortijo J, Milara J, Mata M, Donet E, Gavara N, Peel SE, Hall IP, Morcillo EJ
Number of Citations: 1
(See PubMed article )
  Hu / 1:1000
MA1-934 was used in immunocytochemistry, immunohistochemistry, and western blot to investigate the effect of calcium-sensing receptor on airway epithelial repair

Biochem Pharmacol. 2010 Jul 15;80(2):236-46.
"Extracellular calcium-sensing receptor mediates human bronchial epithelial wound repair."
Author(s): Milara J, Mata M, Serrano A, Peiró T, Morcillo EJ, Cortijo J
Number of Citations: 1
(See PubMed article )
  Hu / 1:100
MA1-934 was used in immunocytochemistry to investigate the effect of thermostable direct hemolysin on colonic carcinoma cell proliferation

PLoS One. 2011;6(5):e20098.
"Thermostable direct hemolysin downregulates human colon carcinoma cell proliferation with the involvement of E-cadherin, and ?-catenin/Tcf-4 signaling."
Author(s): Chowdhury P, Pore D, Mahata N, Karmakar P, Pal A, Chakrabarti MK
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA1-934 was used in immunocytochemistry and immunoprecipitation to investigate the association between calcium-sensing receptor and adaptor protein 14-3-3 and its involvement in Rho kinase signalling

Biochem J. 2012 Feb 1;441(3):995-1006.
"The adaptor protein 14-3-3 binds to the calcium-sensing receptor and attenuates receptor-mediated Rho kinase signalling."
Author(s): Arulpragasam A, Magno AL, Ingley E, Brown SJ, Conigrave AD, Ratajczak T, Ward BK
Number of Citations: 0
(See PubMed article )
  Hu / 1:100
MA1-934 was used in immunocytochemistry and western blot to study the effect of calcimimetic R-568 on nitric oxide release in human endothelial cells

PLoS One. 2012;7(1):e30682.
"Calcimimetic R-568 and its enantiomer S-568 increase nitric oxide release in human endothelial cells."
Author(s): Bonomini M, Giardinelli A, Morabito C, Di Silvestre S, Di Cesare M, Di Pietro N, Sirolli V, Formoso G, Amoroso L, Mariggiò MA, Pandolfi A
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA1-934 was used in immunocytochemistry to study the mechanism for the effect of calcium-sensing receptor on cell adhesion and migration

J Biol Chem. 2011 Nov 25;286(47):40922-33.
"Calcium-sensing receptor modulates cell adhesion and migration via integrins."
Author(s): Tharmalingam S, Daulat AM, Antflick JE, Ahmed SM, Nemeth EF, Angers S, Conigrave AD, Hampson DR
Number of Citations: 1
(See PubMed article )
  Rt / 1:50
MA1-934 was used in immunocytochemistry, immunohistochemistry, and western blot to investigate the role of calcium in dentin formation and its mechanism

Calcif Tissue Int. 2008 Jan;82(1):57-65.
"Identification of a Ca2+-sensing receptor in rat trigeminal ganglia, sensory axons, and tooth dental pulp."
Author(s): Heyeraas KJ, Haug SR, Bukoski RD, Awumey EM
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Bv / Not Cited
Hu / Not Cited
MA1-934 was used in immunohistochemistry and western blot to examine the expression of the calcium-sensing receptor (CaR) in vascular smooth muscle cells

Cardiovasc Res. 2009 Feb 1;81(2):260-8.
"Calcification is associated with loss of functional calcium-sensing receptor in vascular smooth muscle cells."
Author(s): Alam MU, Kirton JP, Wilkinson FL, Towers E, Sinha S, Rouhi M, Vizard TN, Sage AP, Martin D, Ward DT, Alexander MY, Riccardi D, Canfield AE
Number of Citations: 1
(See PubMed article )
  Hu / 1:1000
MA1-934 was used in immunocytochemistry, immunohistochemistry, and western blot to investigate the effect of calcium-sensing receptor on airway epithelial repair

Biochem Pharmacol. 2010 Jul 15;80(2):236-46.
"Extracellular calcium-sensing receptor mediates human bronchial epithelial wound repair."
Author(s): Milara J, Mata M, Serrano A, Peiró T, Morcillo EJ, Cortijo J
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA1-934 was used in immunohistochemistry to evaluate GPRC6A and CaSR receptors as nutrient sensing receptors in gastric endocrine cells

J Mol Histol. 2011 Aug;42(4):355-64.
"Nutrient sensing receptors in gastric endocrine cells."
Author(s): Haid D, Widmayer P, Breer H
Number of Citations: 1
(See PubMed article )
  Hu / 1:100-1:150
MA1-934 was used in immunohistochemistry to study the expression of receptors that respond to protein breakdown products in murine, porcine and human d- and g-cells

Front Physiol. 2012;3():65.
"Receptors responsive to protein breakdown products in g-cells and d-cells of mouse, swine and human."
Author(s): Haid DC, Jordan-Biegger C, Widmayer P, Breer H
Number of Citations: 0
(See PubMed article )
  Ms / Not Cited
MA1-934 was used in immunohistochemistry to investigate the role of calcium-sensing receptor in the vascular calcification in chronic kidney disease

J Nephrol. 2010 Jan-Feb;23(1):17-22.
"Calcium-sensing receptor as a potential modulator of vascular calcification in chronic kidney disease."
Author(s): Caudrillier A, Mentaverri R, Brazier M, Kamel S, Massy ZA
Number of Citations: 1
(See PubMed article )
  Ms / 1:50
MA1-934 was used in immunohistochemistry to investigate the effect of CaSR on CCK release in gut cells

Am J Physiol Gastrointest Liver Physiol. 2011 Apr;300(4):G538-46.
"The extracellular calcium-sensing receptor is required for cholecystokinin secretion in response to L-phenylalanine in acutely isolated intestinal I cells."
Author(s): Liou AP, Sei Y, Zhao X, Feng J, Lu X, Thomas C, Pechhold S, Raybould HE, Wank SA
Number of Citations: 1
(See PubMed article )
  Rt / 10 ug/ml
MA1-934 was used in western blot and immunohistochemistry to investigate the possible regulation of vasodilator actions by CaSR

Circ Res. 2005 Aug 19;97(4):391-8.
"Evidence in favor of a calcium-sensing receptor in arterial endothelial cells: studies with calindol and Calhex 231."
Author(s): Weston AH, Absi M, Ward DT, Ohanian J, Dodd RH, Dauban P, Petrel C, Ruat M, Edwards G
Number of Citations: 1
(See PubMed article )
  Rt / 1:50
MA1-934 was used in immunocytochemistry, immunohistochemistry, and western blot to investigate the role of calcium in dentin formation and its mechanism

Calcif Tissue Int. 2008 Jan;82(1):57-65.
"Identification of a Ca2+-sensing receptor in rat trigeminal ganglia, sensory axons, and tooth dental pulp."
Author(s): Heyeraas KJ, Haug SR, Bukoski RD, Awumey EM
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
MA1-934 was used in immunohistochemistry to evaluate a direct injection method for the introduction of foreign cDNA sequence into parathyroid cells

Endocrinology. 2010 Aug;151(8):4031-8.
"Development of a technique for introduction of an expressed complementary deoxyribonucleic acid into parathyroid cells by direct injection."
Author(s): Shiizaki K, Hatamura I, Fukagawa M, Nakazawa E, Saji F, Watanabe Y, Akizawa T, Kusano E
Number of Citations: 0
(See PubMed article )
  Rt / 1:100
MA1-934 was used in immunohistochemistry to study the ability of a calcium-sensing receptor agonist to attenuate secretagogue-induced intestinal electrolyte secretion

Am J Physiol Gastrointest Liver Physiol. 2012 Jul;303(1):G60-70.
"Calcium-sensing receptor inhibits secretagogue-induced electrolyte secretion by intestine via the enteric nervous system."
Author(s): Cheng SX
Number of Citations: 1
(See PubMed article )
  Rt / 1:100
MA1-934 was used in immunohistochemistry and western blot to study role of enhanced Ca(2+)-sensing receptor function in idiopathic pulmonary arterial hypertension

Circ Res. 2012 Aug 3;111(4):469-81.
"Enhanced Ca(2+)-sensing receptor function in idiopathic pulmonary arterial hypertension."
Author(s): Yamamura A, Guo Q, Yamamura H, Zimnicka AM, Pohl NM, Smith KA, Fernandez RA, Zeifman A, Makino A, Dong H, Yuan JX
Number of Citations: 1
(See PubMed article )
Flow Cytometry
  Species / Dilution Summary
  Hu / 160 ug/ml
MA1-934 was used in ELISA, flow cytometry and western blot to produce and characterize novel anti-human calcium-sensing receptor monoclonal antibodies

J Bone Miner Res. 1997 Nov;12(11):1780-8.
"Monoclonal antibodies against synthetic peptides corresponding to the extracellular domain of the human Ca2+ receptor: characterization and use in studying concanavalin A inhibition."
Author(s): Goldsmith PK, Fan G, Miller JL, Rogers KV, Spiegel AM
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
MA1-934 was used in immunocytochemistry and immunoprecipitation to investigate the association between calcium-sensing receptor and adaptor protein 14-3-3 and its involvement in Rho kinase signalling

Biochem J. 2012 Feb 1;441(3):995-1006.
"The adaptor protein 14-3-3 binds to the calcium-sensing receptor and attenuates receptor-mediated Rho kinase signalling."
Author(s): Arulpragasam A, Magno AL, Ingley E, Brown SJ, Conigrave AD, Ratajczak T, Ward BK
Number of Citations: 0
(See PubMed article )
  Hu / Not Cited
MA1-934 was used in immunoprecipitation and western blot to study the role of extra-parathyroid calcium-sensing receptors in regulating blood calcium concentrations independently of PTH

J Clin Invest. 2012 Sep 4;122(9):3355-67.
"PTH-independent regulation of blood calcium concentration by the calcium-sensing receptor."
Author(s): Loupy A, Ramakrishnan SK, Wootla B, Chambrey R, de la Faille R, Bourgeois S, Bruneval P, Mandet C, Christensen EI, Faure H, Cheval L, Laghmani K, Collet C, Eladari D, Dodd RH, Ruat M, Houillier P
Number of Citations: 4
(See PubMed article )
ELISA
  Species / Dilution Summary
  Hu / 256 ng/ml
MA1-934 was used in ELISA, flow cytometry and western blot to produce and characterize novel anti-human calcium-sensing receptor monoclonal antibodies

J Bone Miner Res. 1997 Nov;12(11):1780-8.
"Monoclonal antibodies against synthetic peptides corresponding to the extracellular domain of the human Ca2+ receptor: characterization and use in studying concanavalin A inhibition."
Author(s): Goldsmith PK, Fan G, Miller JL, Rogers KV, Spiegel AM
Number of Citations: 1
(See PubMed article )
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