CYP27B1 Antibody

CYP27B1 Polyclonal Antibody for Western blot, IHC, FACS

Synonyms:
CP2B, CYP1, CYP1alpha, CYP27B, P450c1, PDDR, VDD1, VDDR, VDDRI, VDR, 1alpha(OH)ase, 25 hydroxyvitamin D3-1-alpha hydroxylase, 25-OHD-1 alpha-hydroxylase, 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial, VD3 1A hydroxylase, calcidiol 1-monooxygenase, cytochrome P450 subfamily XXVIIB polypeptide 1, cytochrome P450C1 alpha, cy
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: KLH conjugated synthetic peptide between 482-508 amino acids from the C-terminal region of human CYP27B1
Ordering Information
Pierce CYP27B1 Antibody
Product #
PA5-26065
Size
400 ul
Price
$316.00
Purchase
Add CYP27B1 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunohistochemistry (IHC) 1:10-1:50
Flow Cytometry (FACS) 1:10-1:50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: Lot-specific
Purification: Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage Buffer: PBS
Preservative: 0.09% sodium azide
Storage Conditions: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
General Information
CYP27B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.
Product Images
  • CYP27B1 Antibody (PA5-26065) in WB
    CYP27B1-_Antibody_PA5-26065_WB_1_112013.jpg

    Western Blot with anti-CYP27B1 Polyclonal Antibody (PA5-26065) (CYP27B1-_Antibody_PA5-26065_WB_1_112013.jpg)

    Western Blot with anti-CYP27B1 Polyclonal Antibody (PA5-26065)

    Western blot analysis using a CYP27B1 polyclonal antibody (Product # PA5-26065) in mouse kidney tissue lysates (35ug per lane).
  • CYP27B1 Antibody (PA5-26065) in IHC
    CYP27B1-_Antibody_PA5-26065_IHC_1_112013.jpg

    Immunohistochemistry with anti-CYP27B1 Polyclonal Antibody (PA5-26065) (CYP27B1-_Antibody_PA5-26065_IHC_1_112013.jpg)

    Immunohistochemistry with anti-CYP27B1 Polyclonal Antibody (PA5-26065)

    Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human kidney tissue using a CYP27B1 polyclonal antibody (Product # PA5-26065), followed by HRP-conjugated secondary antibody and DAB staining.
  • CYP27B1 Antibody (PA5-26065) in FACS
    CYP27B1-_Antibody_PA5-26065_FC_1_112013.jpg

    Flow Cytometry with anti-CYP27B1 Polyclonal Antibody (PA5-26065) (CYP27B1-_Antibody_PA5-26065_FC_1_112013.jpg)

    Flow Cytometry with anti-CYP27B1 Polyclonal Antibody (PA5-26065)

    Flow cytometry analysis of 293 cells using a CYP27B1 polyclonal antibody (Product # PA5-26065) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific