CYLD Antibody

CYLD Polyclonal Antibody for Western blot, IF, ICC, IHC

>> See 4 other antibodies for CYLD (CDMT, CYLD1, CYLDI, EAC, FLJ20180, FLJ31664, FLJ78684, HSPC057, KIAA0849, MFT, MFT1, SBS, TEM, USPL2)
CDMT, CYLD1, CYLDI, EAC, FLJ20180, FLJ31664, FLJ78684, HSPC057, KIAA0849, MFT, MFT1, SBS, TEM, USPL2
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Bovine (Bv), Canine (Ca), Non-human primate (Nhp)
Immunogen: Synthetic peptide corresponding to residues D(32) K Q T Q K L L K V P K G S(45) of human CYLD.
Ordering Information
Pierce CYLD Antibody
Product #
100 µg
Tested Applications Dilution *
Western Blot (WB) 2-4 µg/ml
Immunofluorescence (IF) 5-10 µg/ml
Immunocytochemistry (ICC) 5-10 µg/ml
Immunohistochemistry (IHC) Assay-Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1.0mg/ml
Purification: Protein G
Storage Buffer: PBS
Preservative: 0.05% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
This antibody is predicted to react with rat based on sequence homology.

Suggested positive control: Hela whole cell extract.
General Information
CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.
Product Images
  • CYLD Antibody (PA1-41097) in WB

    Western Blot with anti-CYLD Polyclonal Antibody (PA1-41097) (anti-CYLD-Polyclonal-Antibody-PA1-41097-WB.jpg)

    Western Blot with anti-CYLD Polyclonal Antibody (PA1-41097)

    Western blot analysis of CYLD in HeLa cell lysate using a CYLD polyclonal antibody (Product # PA1-41097) at 2 ug/ml.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific