CYLD Antibody

CYLD Polyclonal Antibody for Western blot, IF, ICC, IHC

>> See 3 other antibodies for CYLD (CDMT, CYLD1, CYLDI, EAC, FLJ20180, FLJ31664, FLJ78684, HSPC057, KIAA0849, MFT, MFT1, SBS, TEM, USPL2)
Synonyms:
CDMT, CYLD1, CYLDI, EAC, FLJ20180, FLJ31664, FLJ78684, HSPC057, KIAA0849, MFT, MFT1, SBS, TEM, USPL2
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Bovine (Bv), Canine (Ca), Non-human primate (Nhp)
Immunogen: Synthetic peptide corresponding to residues D(32) K Q T Q K L L K V P K G S(45) of human CYLD.
Ordering Information
Pierce CYLD Antibody
Product #
PA1-41097
Size
100 µg
Price
$350.00
Purchase
Add CYLD Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 2-4 µg/ml
Immunofluorescence (IF) 5-10 µg/ml
Immunocytochemistry (ICC) 5-10 µg/ml
Immunohistochemistry (IHC) Assay-Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.5mg/ml
Purification: Protein G
Storage Buffer: PBS with 0.05% BSA
Preservative: 0.05% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
This antibody is predicted to react with rat based on sequence homology.

Suggested positive control: Hela whole cell extract.
General Information
CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.
Product Images
  • CYLD Antibody (PA1-41097) in WB
    anti-CYLD-Polyclonal-Antibody-PA1-41097-WB.jpg

    Western Blot with anti-CYLD Polyclonal Antibody (PA1-41097) (anti-CYLD-Polyclonal-Antibody-PA1-41097-WB.jpg)

    Western Blot with anti-CYLD Polyclonal Antibody (PA1-41097)

    Western blot analysis of CYLD in HeLa cell lysate using a CYLD polyclonal antibody (Product # PA1-41097) at 2 ug/ml.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific