CRYAB Antibody (1D11C6E6)

CRYAB Monoclonal Antibody for Western blot, IHC (P)

>> See 8 other antibodies for CRYAB (CRYA2; CTPP2; HSPB5; CRYAB)
Synonyms:
CRYA2; CTPP2; HSPB5; CRYAB
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG2a
Class: Monoclonal
Type: Antibody
Clone: 1D11C6E6
Tested Species Reactivity: Human (Hu)
Immunogen: Purified recombinant fragment of CRYAB (aa1-175) expressed in E. Coli.
Ordering Information
Pierce CRYAB Antibody (1D11C6E6)
Product #
MA5-15383
Size
100 ul
Price
$316.00
Purchase
Add CRYAB Antibody (1D11C6E6) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1/500 - 1/2000
Immunohistochemistry (Paraffin) (IHC (P)) 1/200 - 1/1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: ascites
Preservative: 0.03% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
MA5-15383 targets CRYAB in IHC and WB applications and shows reactivity with Human samples.

The MA5-15383 immunogen is purified recombinant fragment of CRYAB (aa1-175) expressed in E. Coli.
General Information
Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
Product Images
  • CRYAB Antibody (MA5-15383) in WB
    CRYAB_Antibody_MA5-15383_Western-blot_2_20131210104422.jpg

    Western Blot with anti-CRYAB Monoclonal Antibody [1D11C6E6] (MA5-15383) (CRYAB_Antibody_MA5-15383_Western-blot_2_20131210104422.jpg)

    Western Blot with anti-CRYAB Monoclonal Antibody [1D11C6E6] (MA5-15383)

    Western blot analysis of CRYAB was performed by loading 5ug of human brain tumor tissue lysates per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% milk in TBST for 20 minutes. The membrane was probed with a CRYAB monoclonal antibody (Product # MA5-15383) at a dilution of 1:500 overnight at 4ºC, washed in TBS + 0.1% Tween-20, and probed with an HRP-conjugated anti-mouse IgG secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature. Detection was performed using a chemiluminescent substrate. Data courtesy of the Innovators Program.
  • CRYAB Antibody (MA5-15383) in WB
    CRYAB_Antibody_MA5-15383_Westernblot1-1252013.jpg

    Western Blot with anti-CRYAB Monoclonal Antibody [1D11C6E6] (MA5-15383) (CRYAB_Antibody_MA5-15383_Westernblot1-1252013.jpg)

    Western Blot with anti-CRYAB Monoclonal Antibody [1D11C6E6] (MA5-15383)

    Western blot analysis of CRYAB using CRYAB monoclonal antibody (Product # MA5-15383) in HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY CRYAB cDNA (2).
  • CRYAB Antibody (MA5-15383) in IHC
    CRYAB_Antibody_MA5-15383_Immunohistochemical2-1252013.jpg

    Immunohistochemistry with anti-CRYAB Monoclonal Antibody [1D11C6E6] (MA5-15383) (CRYAB_Antibody_MA5-15383_Immunohistochemical2-1252013.jpg)

    Immunohistochemistry with anti-CRYAB Monoclonal Antibody [1D11C6E6] (MA5-15383)

    Immunohistochemical analysis of paraffin-embedded human brain hippocampus (A), lung cancer (B), brain tumor (C), breast cance (D) using CRYAB monoclonal antibody (Product # MA5-15383) followed with DAB staining.
  • CRYAB Antibody (MA5-15383) in IHC
    CRYAB_Antibody_MA5-15383_Immunohistochemical1-1252013.jpg

    Immunohistochemistry with anti-CRYAB Monoclonal Antibody [1D11C6E6] (MA5-15383) (CRYAB_Antibody_MA5-15383_Immunohistochemical1-1252013.jpg)

    Immunohistochemistry with anti-CRYAB Monoclonal Antibody [1D11C6E6] (MA5-15383)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissues using CRYAB monoclonal antibody (Product # MA5-15383).
  • CRYAB Antibody (MA5-15383) in IHC (P)
    CRYAB_Antibody_MA5-15383_Immunohistochemistry_3_20131210104357.jpg

    Immunohistochemistry (Paraffin) with anti-CRYAB Monoclonal Antibody [1D11C6E6] (MA5-15383) (CRYAB_Antibody_MA5-15383_Immunohistochemistry_3_20131210104357.jpg)

    Immunohistochemistry (Paraffin) with anti-CRYAB Monoclonal Antibody [1D11C6E6] (MA5-15383)

    Immunohistochemistry was performed on formalin-fixed, paraffin-embedded human brain tumor tissue sections. To expose target antigens, heat-induced epitope retrieval was performed by heating tissues at 120ºC for 5 minutes in sodium citrate buffer pH 6.0. Tissues were blocked in 3% hydrogen peroxide for 5 minutes at room temperature and probed with a CRYAB monoclonal antibody (Product # MA5-15383) at a dilution of 1:500 for 80 minutes at room temperature. Tissues were washed extensively with TBST. Detection was performed using an HRP-conjugated anti-mouse IgG secondary antibody and colorimetric substrate. Tissues were counterstained with hematoxylin and visualized by light microscopy. Data courtesy of the Innovators Program.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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