CLN2 Polyclonal antibody for Western blot, IF, ICC, IHC (P)
GIG1, CLN2, LPIC, TPP-1, cell growth-inhibiting gene 1 protein, growth-inhibiting protein 1, lysosomal pepstatin insensitive protease, tripeptidyl aminopeptidase, tripeptidyl-peptidase 1
Host / Isotype: Rabbit / IgG
Tested Species Reactivity: Human (Hu)
Immunogen: Recombinant fragment corresponding to a region within amino acids 66 and 324 of TPP1 (Uniprot ID#O14773)
Western Blot (WB)
Immunohistochemistry (Paraffin) (IHC (P))
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.1M tris, pH 7,
10% glycerol, 0.1M glycine
-20° C, Avoid Freeze/Thaw Cycles
PA5-22274 targets CLN2 in ICC, IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-22274 immunogen is a recombinant fragment corresponding to a region within amino acids 66 and 324 of TPP1 (Uniprot ID#O14773).
CLN2 antibody (PA5-22274) in WB
Western Blot with anti-CLN2 Polyclonal antibody (PA5-22274)
Western blot analysis in whole cell lysates of (target) in lanes A). A431 cells [30 ug] B). H1299 cells [30 ug] C). Hela cells [30 ug] using PA5-22274 anti-CLN2 primary antibody at a dilution of 1:1000
CLN2 antibody (PA5-22274) in IF
Immunofluorescence with anti-CLN2 Polyclonal antibody (PA5-22274)
Immunofluorescence analysis of methanol-fixed A431 cells using PA5-22274 anti-TPP1 antibody at 1:200 dilution.
CLN2 antibody (PA5-22274) in IHC
Immunohistochemistry with anti-CLN2 Polyclonal antibody (PA5-22274)
Immunohistochemical analysis of paraffin-embedded SW480 xenograft tissue using PA5-22274 anti-TPP1 antibody at 1:100 dilution.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)