CHAT Antibody

CHAT Polyclonal Antibody for Western blot, IF

>> See 10 other antibodies for CHAT (CHOACTASE, CMS1A, CMS1A2, acetyl CoA:choline O-acetyltransferase, choline acetylase)
Synonyms:
CHOACTASE, CMS1A, CMS1A2, acetyl CoA:choline O-acetyltransferase, choline acetylase
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Synthetic peptide from the N-terminal region of human CHAT conjugated to KLH.
Ordering Information
Pierce CHAT Antibody
Product #
PA5-26597
Size
400 ul
Price
$316.00
Purchase
Add CHAT Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunofluorescence (IF) 1:10-50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: Lot-specific
Purification: Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage Buffer: PBS
Preservative: 0.09% sodium azide
Storage Conditions: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
PA5-26597 targets CHAT in IF and WB applications and shows reactivity with Human and mouse samples.

The PA5-26597 immunogen is synthetic peptide from the N-terminal region of human CHAT conjugated to KLH.
General Information
Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).
Product Images
  • CHAT Antibody (PA5-26597) in WB
    CHAT_Antibody_PA5-26597_WB_2_112013.jpg

    Western Blot with anti-CHAT Polyclonal Antibody (PA5-26597) (CHAT_Antibody_PA5-26597_WB_2_112013.jpg)

    Western Blot with anti-CHAT Polyclonal Antibody (PA5-26597)

    Western blot analysis of anti-CHAT Polyclonal Antibody (PA5-26597) in mouse liver tissue lysates (35 ug per lane).
  • CHAT Antibody (PA5-26597) in WB
    CHAT_Antibody_PA5-26597_WB_1_112013.jpg

    Western Blot with anti-CHAT Polyclonal Antibody (PA5-26597) (CHAT_Antibody_PA5-26597_WB_1_112013.jpg)

    Western Blot with anti-CHAT Polyclonal Antibody (PA5-26597)

    Western blot analysis of anti-CHAT Polyclonal Antibody (PA5-26597) in NCI-H460 cell line lysates (35 ug per lane).
  • CHAT Antibody (PA5-26597) in IF
    CHAT_Antibody_PA5-26597_IF_1_112013.jpg

    Immunofluorescence with anti-CHAT Polyclonal Antibody (PA5-26597) (CHAT_Antibody_PA5-26597_IF_1_112013.jpg)

    Immunofluorescence with anti-CHAT Polyclonal Antibody (PA5-26597)

    Immunofluorescent analysis of NCI-H460 cells using anti-CHAT Polyclonal Antibody (PA5-26597) at a dilution of 1:10-50. Primary antibody was detected with goat anti-rabbit lgG, Alexa Fluor 488 conjugated secondary antibody (green). DAPI was used to stain the cells nuclear (blue).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific