CFTR Antibody (CF3)

CFTR Monoclonal Antibody for Western blot, IF, ICC, IHC, FACS, IP

>> See 4 other antibodies for CFTR (Cystic Fibrosis Transmembrane Conductance Regulator)
Synonyms:
Cystic Fibrosis Transmembrane Conductance Regulator
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgM
Class: Monoclonal
Type: Antibody
Clone: CF3
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Published Species Reactivity: Chicken (Ck), Human (Hu), Mouse (Ms), Rat (Rt)
Immunogen: Synthetic Peptide: G(103) R I I A S Y D P D N K E E R(117)
Ordering Information
Pierce CFTR Antibody (CF3)
Product #
MA1-935
Size
100 µl
Price
$360.00
Purchase
Add CFTR Antibody (CF3) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500
Immunofluorescence (IF) 1:500
Immunocytochemistry (ICC) Assay Dependent
Immunohistochemistry (IHC) 1:200
Flow Cytometry (FACS) 1/100
Immunoprecipitation (IP) Assay dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Flow Cytometry (FACS) See publications below
Immunoprecipitation (IP) See publications below
Blocking Assay (BLOCK) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: ascites
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA1-935 detects cystic fibrosis transmembrane conductance factor (CFTR) in human and mouse tissues.

MA1-935 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a single ~170 kDa protein representing CFTR in T84 whole cell extract. Immunofluorescence staining of CFTR in mouse epithelial cells with MA1-935 results in cell surface staining, consistent with localization at the plasma membrane. This antibody also detects one or more immunologically related proteins in mouse cell line Heb7a that does not contain CFTR mRNA. MA1-935 can also be used to inhibit the epithelial uptake of S. typhi in some mouse cell lines.

MA1-935 immunizing peptide corresponds to amino acid residues 103-117 found in the first extracellular loop of human and rabbit CFTR. This sequence is highly conserved in mouse, sheep, cow, and Xenopus laevis.
General Information
Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508) which results in abnormal chloride transport.

Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is. One possible explanation is that Salmonella typhi has been shown to use CFTR to enter intestinal epithelial cells and that delta F508 heterozygote and homozygote mice showed 86% and 100% reductions in S. typhi intestinal submucosal uptake.
Product Images
  • CFTR Antibody (MA1-935) in IF
    MA1-935_IF_Hela.jpg

    Immunofluorescence with anti-CFTR Monoclonal Antibody [CF3] (MA1-935) (MA1-935_IF_Hela.jpg)

    Immunofluorescence with anti-CFTR Monoclonal Antibody [CF3] (MA1-935)

    Immunofluorescent analysis of CFTR using CFTR Monoclonal antibody (CF3) (Product# MA1-935) shows staining in HeLa cells. CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR (Product# MA1-935) at a dilution of 1:100-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
  • CFTR Antibody (MA1-935) in IF
    MA1-935_IF_U251.jpg

    Immunofluorescence with anti-CFTR Monoclonal Antibody [CF3] (MA1-935) (MA1-935_IF_U251.jpg)

    Immunofluorescence with anti-CFTR Monoclonal Antibody [CF3] (MA1-935)

    Immunofluorescent analysis of CFTR using CFTR Monoclonal antibody (CF3) (Product# MA1-935) shows staining in U251 glioma cells. CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR (Product# MA1-935) at a dilution of 1:100-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
  • CFTR Antibody (MA1-935) in IF
    MA1-935_IF_WiDr.jpg

    Immunofluorescence with anti-CFTR Monoclonal Antibody [CF3] (MA1-935) (MA1-935_IF_WiDr.jpg)

    Immunofluorescence with anti-CFTR Monoclonal Antibody [CF3] (MA1-935)

    Immunofluorescent analysis of CFTR using CFTR Monoclonal antibody (CF3) (Product# MA1-935) shows staining in WiDr colon carcinoma cells. CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR (Product# MA1-935) at a dilution of 1:100-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
  • CFTR Antibody (MA1-935) in IHC
    MA1-935_IHC_colon_carcinoma-tissue.jpg

    Immunohistochemistry with anti-CFTR Monoclonal Antibody [CF3] (MA1-935) (MA1-935_IHC_colon_carcinoma-tissue.jpg)

    Immunohistochemistry with anti-CFTR Monoclonal Antibody [CF3] (MA1-935)

    Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing CFTR (MA1-935) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • CFTR Antibody (MA1-935) in IHC
    MA1-935_IHC_Pancreas_Tissue.jpg

    Immunohistochemistry with anti-CFTR Monoclonal Antibody [CF3] (MA1-935) (MA1-935_IHC_Pancreas_Tissue.jpg)

    Immunohistochemistry with anti-CFTR Monoclonal Antibody [CF3] (MA1-935)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human pancreas tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing CFTR (MA1-935) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • CFTR Antibody (MA1-935) in IHC
    MA1-935_IHC_Tonsil_Tissue.jpg

    Immunohistochemistry with anti-CFTR Monoclonal Antibody [CF3] (MA1-935) (MA1-935_IHC_Tonsil_Tissue.jpg)

    Immunohistochemistry with anti-CFTR Monoclonal Antibody [CF3] (MA1-935)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing CFTR (MA1-935) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Ck / Not Cited
MA1-935 was used in western blot to study the effect of the parathyroid hormone on a sodium/proton exchanger and a chloride channel in the chick proximal tubule.

Am J Physiol Renal Physiol. 2003 May;284(5):F987-95.
"PTH stimulates a Cl(-)-dependent and EIPA-sensitive current in chick proximal tubule cells in culture."
Author(s): Laverty G, McWilliams C, Sheldon A, Arnason SS
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA1-935 was used in immunocytochemistry and western blot to produce and characterize novel monoclonal and polyclonal antibodies to CFTR

J Cell Sci. 1995 Jun;108 ( Pt 6)():2433-44.
"Production and characterisation of monoclonal and polyclonal antibodies to different regions of the cystic fibrosis transmembrane conductance regulator (CFTR): detection of immunologically related proteins."
Author(s): Walker J, Watson J, Holmes C, Edelman A, Banting G
Number of Citations: 12
(See PubMed article )
  Hu / Not Cited
MA1-935 was used in blocking/activating experiment and western blot to study the role of CFTR in the epithelial entrance of Salmonella typhi

Nature. 1998 May 7;393(6680):79-82.
"Salmonella typhi uses CFTR to enter intestinal epithelial cells."
Author(s): Pier GB, Grout M, Zaidi T, Meluleni G, Mueschenborn SS, Banting G, Ratcliff R, Evans MJ, Colledge WH
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA1-935 was used in western blot and immunohistochemistry to investigate the impact of the location of specific tropomyosin isoforms on CFTR's position.

Mol Biol Cell. 2003 Nov;14(11):4365-75.
"Polarization of specific tropomyosin isoforms in gastrointestinal epithelial cells and their impact on CFTR at the apical surface."
Author(s): Dalby-Payne JR, O'Loughlin EV, Gunning P
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA1-935 was used in immunocytochemistry, immunoprecipitation, and western blot to review the suitability of currently available antibodies for the detection of the cystic fibrosis conductance regulator

J Cyst Fibros. 2004 Aug;3 Suppl 2():69-72.
"Antibodies for CFTR studies."
Author(s): Mendes F, Farinha CM, Roxo-Rosa M, Fanen P, Edelman A, Dormer R, McPherson M, Davidson H, Puchelle E, De Jonge H, Heda GD, Gentzsch M, Lukacs G, Penque D, Amaral MD
Number of Citations: 9
(See PubMed article )
  Hu / 0
MA1-935 was used in immunohistochemistry, western blot, and western blot to investigate the role of cystic fibrosis transmembrane conductance regulator in the rat endocrine pancreas

Endocrine. 2007 Oct;32(2):197-205.
"Expression and localization of cystic fibrosis transmembrane conductance regulator in the rat endocrine pancreas."
Author(s): Boom A, Lybaert P, Pollet JF, Jacobs P, Jijakli H, Golstein PE, Sener A, Malaisse WJ, Beauwens R
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA1-935 was used in blocking/activating experiment and western blot to study the role of CFTR in the epithelial entrance of Salmonella typhi

Nature. 1998 May 7;393(6680):79-82.
"Salmonella typhi uses CFTR to enter intestinal epithelial cells."
Author(s): Pier GB, Grout M, Zaidi T, Meluleni G, Mueschenborn SS, Banting G, Ratcliff R, Evans MJ, Colledge WH
Number of Citations: 1
(See PubMed article )
  Rt / 0
MA1-935 was used in immunohistochemistry, western blot, and western blot to investigate the role of cystic fibrosis transmembrane conductance regulator in the rat endocrine pancreas

Endocrine. 2007 Oct;32(2):197-205.
"Expression and localization of cystic fibrosis transmembrane conductance regulator in the rat endocrine pancreas."
Author(s): Boom A, Lybaert P, Pollet JF, Jacobs P, Jijakli H, Golstein PE, Sener A, Malaisse WJ, Beauwens R
Number of Citations: 1
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / Not Cited
MA1-935 was used in immunocytochemistry and western blot to produce and characterize novel monoclonal and polyclonal antibodies to CFTR

J Cell Sci. 1995 Jun;108 ( Pt 6)():2433-44.
"Production and characterisation of monoclonal and polyclonal antibodies to different regions of the cystic fibrosis transmembrane conductance regulator (CFTR): detection of immunologically related proteins."
Author(s): Walker J, Watson J, Holmes C, Edelman A, Banting G
Number of Citations: 12
(See PubMed article )
  Hu / Not Cited
MA1-935 was used in immunocytochemistry, immunoprecipitation, and western blot to review the suitability of currently available antibodies for the detection of the cystic fibrosis conductance regulator

J Cyst Fibros. 2004 Aug;3 Suppl 2():69-72.
"Antibodies for CFTR studies."
Author(s): Mendes F, Farinha CM, Roxo-Rosa M, Fanen P, Edelman A, Dormer R, McPherson M, Davidson H, Puchelle E, De Jonge H, Heda GD, Gentzsch M, Lukacs G, Penque D, Amaral MD
Number of Citations: 9
(See PubMed article )
  Hu / 1:50
MA1-935 was used in immunocytochemistry to study the effect of IFNgamma on CFTR expression in mast cells and epithelial cells

J Pharmacol Exp Ther. 2005 Nov;315(2):563-70.
"Differential regulation of cystic fibrosis transmembrane conductance regulator by interferon gamma in mast cells and epithelial cells."
Author(s): Kulka M, Dery R, Nahirney D, Duszyk M, Befus AD
Number of Citations: 1
(See PubMed article )
  Rt / 1:100
MA1-935 was used in flow cytometry and immunocytochemistry to investigate the role of cystic fibrosis transmembrane conductance regulator (CFTR) in mast cells

J Leukoc Biol. 2002 Jan;71(1):54-64.
"Expression and functional characterization of CFTR in mast cells."
Author(s): Kulka M, Gilchrist M, Duszyk M, Befus AD
Number of Citations: 2
(See PubMed article )
  Rt / 1:50
MA1-935 was used in immunocytochemistry to study the effect of IFNgamma on CFTR expression in mast cells and epithelial cells

J Pharmacol Exp Ther. 2005 Nov;315(2):563-70.
"Differential regulation of cystic fibrosis transmembrane conductance regulator by interferon gamma in mast cells and epithelial cells."
Author(s): Kulka M, Dery R, Nahirney D, Duszyk M, Befus AD
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / Not Cited
MA1-935 was used in western blot and immunohistochemistry to investigate the impact of the location of specific tropomyosin isoforms on CFTR's position.

Mol Biol Cell. 2003 Nov;14(11):4365-75.
"Polarization of specific tropomyosin isoforms in gastrointestinal epithelial cells and their impact on CFTR at the apical surface."
Author(s): Dalby-Payne JR, O'Loughlin EV, Gunning P
Number of Citations: 1
(See PubMed article )
  Hu / 1 ug/ml
MA1-935 was used in immunohistochemistry to investigate the possible effect of CFTR mutations in pancreatic and tracheal cells on activation of apoptosis

FASEB J. 2007 Sep;21(11):2939-48.
"Exaggerated apoptosis and NF-kappaB activation in pancreatic and tracheal cystic fibrosis cells."
Author(s): Rottner M, Kunzelmann C, Mergey M, Freyssinet JM, Martínez MC
Number of Citations: 1
(See PubMed article )
  Rt / 1:1000
MA1-935 was used in immunohistochemistry, western blot, and western blot to investigate the role of cystic fibrosis transmembrane conductance regulator in the rat endocrine pancreas

Endocrine. 2007 Oct;32(2):197-205.
"Expression and localization of cystic fibrosis transmembrane conductance regulator in the rat endocrine pancreas."
Author(s): Boom A, Lybaert P, Pollet JF, Jacobs P, Jijakli H, Golstein PE, Sener A, Malaisse WJ, Beauwens R
Number of Citations: 1
(See PubMed article )
Flow Cytometry
  Species / Dilution Summary
  Hu / Not Cited
Rt / Not Cited
MA1-935 was used in flow cytometry and immunocytochemistry to investigate the role of cystic fibrosis transmembrane conductance regulator (CFTR) in mast cells

J Leukoc Biol. 2002 Jan;71(1):54-64.
"Expression and functional characterization of CFTR in mast cells."
Author(s): Kulka M, Gilchrist M, Duszyk M, Befus AD
Number of Citations: 2
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
MA1-935 was used in immunocytochemistry, immunoprecipitation, and western blot to review the suitability of currently available antibodies for the detection of the cystic fibrosis conductance regulator

J Cyst Fibros. 2004 Aug;3 Suppl 2():69-72.
"Antibodies for CFTR studies."
Author(s): Mendes F, Farinha CM, Roxo-Rosa M, Fanen P, Edelman A, Dormer R, McPherson M, Davidson H, Puchelle E, De Jonge H, Heda GD, Gentzsch M, Lukacs G, Penque D, Amaral MD
Number of Citations: 9
(See PubMed article )
Blocking Assay
  Species / Dilution Summary
  Hu / 1:10
MA1-935 was used in blocking/activating experiment and western blot to study the role of CFTR in the epithelial entrance of Salmonella typhi

Nature. 1998 May 7;393(6680):79-82.
"Salmonella typhi uses CFTR to enter intestinal epithelial cells."
Author(s): Pier GB, Grout M, Zaidi T, Meluleni G, Mueschenborn SS, Banting G, Ratcliff R, Evans MJ, Colledge WH
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA1-935 was used in blocking or activating experiment to study the mechanism for Salmonella enterica serovar Typhi adhesion with BHK epithelial cells

Microb Pathog. 2011 Nov;51(5):373-7.
"Type IV(B) pili are required for invasion but not for adhesion of Salmonella enterica serovar Typhi into BHK epithelial cells in a cystic fibrosis transmembrane conductance regulator-independent manner."
Author(s): Bravo D, Blondel CJ, Hoare A, Leyton L, Valvano MA, Contreras I
Number of Citations: 1
(See PubMed article )
  Ms / 1:10
MA1-935 was used in blocking/activating experiment and western blot to study the role of CFTR in the epithelial entrance of Salmonella typhi

Nature. 1998 May 7;393(6680):79-82.
"Salmonella typhi uses CFTR to enter intestinal epithelial cells."
Author(s): Pier GB, Grout M, Zaidi T, Meluleni G, Mueschenborn SS, Banting G, Ratcliff R, Evans MJ, Colledge WH
Number of Citations: 1
(See PubMed article )
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