CEBP alpha Antibody (15C8)

CEBP alpha Monoclonal Antibody for Western blot, IP

>> See 4 other antibodies for CEBPA (CCAAT/Enhancer Binding Protein Alpha)
Synonyms:
CCAAT/Enhancer Binding Protein Alpha
Entrez Gene ID:
Details
Host / Isotype: Mouse / IgG
Class: Monoclonal
Type: Antibody
Clone: 15C8
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Published Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Immunogen: Synthetic peptide corresponding to residues M(1) E S A D F Y E V E P R P P(14) of mouse C/EBP alpha.
Ordering Information
Pierce CEBP alpha Antibody (15C8)
Product #
MA1-825
Size
100 µg
Price
$316.00
Purchase
Add CEBP alpha Antibody (15C8) to your cart.
Tested Applications Dilution *
Western Blot (WB) 2 ug/ml
Immunoprecipitation (IP) Assay dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Protein G
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA1-825 detects C/EBP alpha protein in human, mouse and rat samples.

MA1-825 has successfully been used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 45 kDa protein representing C/EBP alpha in transfected hu293T cells. MA1-825 is specific to the amino terminal region of C/EBP alpha.

The MA1-825 immunogen is a synthetic peptide corresponding to residues M(1) E S A D F Y E V E P R P P(14) of mouse C/EBP alpha. This peptide (Cat. # PEP-242) is available for use in neutralization and control experiments.

General Information
The C/EBP (CCAAT/enhancer binding protein) family of transcription factors--alpha, beta, delta, and epsilon)--are widely expressed proteins found in a variety of tissues.  These domains contain an evolutionarily conserved five-amino acid motif (the regulatory domain motif RDM) that conforms to the consensus sequence (I/V/L) KXEP.  One of the major roles of these transcription factors is that of metabolic regulation in liver and fat cells.  Studies suggest that these transcription factors act in a cascade aiding in the development of the adipose cell. Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation and display different function in gene regulation.  From a neurological standpoint, C/EBPs have been implicated in the mediation of nerve growth factor, altering synaptic plasticity, and mediation of inflammatory response in AD patients.
Product Images
  • CEBP alpha Antibody (MA1-825) in WB
    MA1-825_1_07232010.jpg

    Western Blot with anti-CEBP alpha Monoclonal Antibody [15C8] (MA1-825) (MA1-825_1_07232010.jpg)

    Western Blot with anti-CEBP alpha Monoclonal Antibody [15C8] (MA1-825)

    Western blot detection of C/EBP alpha on transfected 293T cells using MA1-825.
Publications:
Western Blot
  Species / Dilution Summary
  Ms / Not Cited
MA1-825 was used in western blot to study the interaction between C/EBPalpha and nuclear factor-kappaB p50

Mol Cancer Res. 2005 Oct;3(10):585-96.
"CCAAT/enhancer binding protein alpha (C/EBPalpha) and C/EBPalpha myeloid oncoproteins induce bcl-2 via interaction of their basic regions with nuclear factor-kappaB p50."
Author(s): Paz-Priel I, Cai DH, Wang D, Kowalski J, Blackford A, Liu H, Heckman CA, Gombart AF, Koeffler HP, Boxer LM, Friedman AD
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
MA1-825 was used in immunoprecipitation and western blot to investigate the interactions of C/EBPalpha or C/EBPalpha with NF-kappaB p50 and their effect on apoptosis

Leukemia. 2009 Feb;23(2):365-74.
"C/EBPalpha or C/EBPalpha oncoproteins regulate the intrinsic and extrinsic apoptotic pathways by direct interaction with NF-kappaB p50 bound to the bcl-2 and FLIP gene promoters."
Author(s): Paz-Priel I, Ghosal AK, Kowalski J, Friedman AD
Number of Citations: 1
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / Not Cited
MA1-825 was used in immunocytochemistry to investigate the role of C/EBP-alpha on the regulation of rRNA synthesis and cell growth and proliferation

EMBO J. 2010 Mar 3;29(5):897-909.
"Nucleolar retention of a translational C/EBPalpha isoform stimulates rDNA transcription and cell size."
Author(s): Müller C, Bremer A, Schreiber S, Eichwald S, Calkhoven CF
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Rt / Not Cited
MA1-825 was used in immunoprecipitation and western blot to investigate the interactions of C/EBPalpha or C/EBPalpha with NF-kappaB p50 and their effect on apoptosis

Leukemia. 2009 Feb;23(2):365-74.
"C/EBPalpha or C/EBPalpha oncoproteins regulate the intrinsic and extrinsic apoptotic pathways by direct interaction with NF-kappaB p50 bound to the bcl-2 and FLIP gene promoters."
Author(s): Paz-Priel I, Ghosal AK, Kowalski J, Friedman AD
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific