CD324 / E Cadherin Antibody

CD324 / E Cadherin Polyclonal Antibody for Western blot, FACS

>> See 26 other antibodies for CDH1 (E-cadherin; Uvomorulin; Cadherin-1; CAM 120/80)
Synonyms:
E-cadherin; Uvomorulin; Cadherin-1; CAM 120/80
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu )
Immunogen: Synthetic peptide from the N-terminal region of CDH1 conjugated to KLH.
Ordering Information
Pierce CD324 / E Cadherin Antibody
Product #
PA5-11548
Size
400 ul
Price
$316.00
Purchase
Add CD324 / E Cadherin Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Flow Cytometry (FACS) 1:10 - 1:50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: Lot-specific
Purification: Protein A
Storage Buffer: PBS
Preservative: 0.09% sodium azide
Storage Conditions: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
PA5-11548 targets E Cadherin in Western blot and flow cytometry applications and shows reactivity with Human samples.

The PA5-11548 immunogen is a synthetic peptide from the N-terminal region of CDH1 conjugated to KLH.

PA5-11548 detects E Cadherin which has a predicted molecular weight of approximately 97 kDa.
General Information
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.
Product Images
  • CD324 / E Cadherin Antibody (PA5-11548) in WB
    E-Cadherin_Antibody_PA5-11548_WB_2_112013.jpg

    Western Blot with anti-CD324  /  E Cadherin Polyclonal Antibody (PA5-11548) (E-Cadherin_Antibody_PA5-11548_WB_2_112013.jpg)

    Western Blot with anti-CD324 / E Cadherin Polyclonal Antibody (PA5-11548)

    Western blot analysis of CDH1 (arrow) using anti-E Cadherin Polyclonal Antibody (PA5-11548). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH1 gene (Lane 2).
  • CD324 / E Cadherin Antibody (PA5-11548) in WB
    E-Cadherin_Antibody_PA5-11548_WB_1_112013.jpg

    Western Blot with anti-CD324  /  E Cadherin Polyclonal Antibody (PA5-11548) (E-Cadherin_Antibody_PA5-11548_WB_1_112013.jpg)

    Western Blot with anti-CD324 / E Cadherin Polyclonal Antibody (PA5-11548)

    Western blot analysis of anti-E Cadherin Polyclonal Antibody (PA5-11548) in 293 cell line lysates (35 ug per lane).
  • CD324 / E Cadherin Antibody (PA5-11548) in FACS
    E-Cadherin_Antibody_PA5-11548_FC_1_112013.jpg

    Flow Cytometry with anti-CD324  /  E Cadherin Polyclonal Antibody (PA5-11548) (E-Cadherin_Antibody_PA5-11548_FC_1_112013.jpg)

    Flow Cytometry with anti-CD324 / E Cadherin Polyclonal Antibody (PA5-11548)

    Flow cytometric analysis of NCI-H292 cells using anti-E Cadherin Polyclonal Antibody (PA5-11548) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50. Secondary antibody used to visualize was a Goat Anti-Rabbit Secondary Antibody Conjugated to FITC.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific