CD324 / E Cadherin Antibody

CD324 / E Cadherin Polyclonal Antibody for Western blot, FACS

>> See 28 other antibodies for CDH1 (E-cadherin; Uvomorulin; Cadherin-1; CAM 120/80)
Synonyms:
E-cadherin; Uvomorulin; Cadherin-1; CAM 120/80
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Immunogen: KLH conjugated synthetic peptide between 160-189 amino acids from the N-terminal region of human E Cadherin (CDH1)
Ordering Information
Pierce CD324 / E Cadherin Antibody
Product #
PA5-11548
Size
400 ul
Price
$316.00
Purchase
Add CD324 / E Cadherin Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Flow Cytometry (FACS) 1:10-1:50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: Lot-specific
Purification: Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage Buffer: PBS
Preservative: 0.09% sodium azide
Storage Conditions: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
General Information
CDH1 is a classical cadherin from the cadherin superfamily. This protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization.
Product Images
  • CD324 / E Cadherin Antibody (PA5-11548) in WB
    E-Cadherin_Antibody_PA5-11548_WB_2_112013.jpg

    Western Blot with anti-CD324 / E Cadherin Polyclonal Antibody (PA5-11548) (E-Cadherin_Antibody_PA5-11548_WB_2_112013.jpg)

    Western Blot with anti-CD324 / E Cadherin Polyclonal Antibody (PA5-11548)

    Western blot analysis of CDH1 (arrow) using an E Cadherin polyclonal antibody (Product # PA5-11548) in 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH1 gene (Lane 2).
  • CD324 / E Cadherin Antibody (PA5-11548) in WB
    E-Cadherin_Antibody_PA5-11548_WB_1_112013.jpg

    Western Blot with anti-CD324 / E Cadherin Polyclonal Antibody (PA5-11548) (E-Cadherin_Antibody_PA5-11548_WB_1_112013.jpg)

    Western Blot with anti-CD324 / E Cadherin Polyclonal Antibody (PA5-11548)

    Western blot analysis using an E Cadherin polyclonal antibody (Product # PA5-11548) in 293 cell lysates (35ug per lane).
  • CD324 / E Cadherin Antibody (PA5-11548) in FACS
    E-Cadherin_Antibody_PA5-11548_FC_1_112013.jpg

    Flow Cytometry with anti-CD324 / E Cadherin Polyclonal Antibody (PA5-11548) (E-Cadherin_Antibody_PA5-11548_FC_1_112013.jpg)

    Flow Cytometry with anti-CD324 / E Cadherin Polyclonal Antibody (PA5-11548)

    Flow cytometry analysis of NCI-H292 cells using an E Cadherin polyclonal antibody (Product # PA5-11548) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific