CD262 / TRAIL Receptor 2 DR5 Antibody

CD262 / TRAIL Receptor 2 DR5 Polyclonal Antibody for Western blot

>> See 9 other antibodies for TNFRSF10B (Apo-2L, HLA class II histocompatibility antigen DRB1-11 beta chain, MHC class I antigen DRB1*11, DR-5, DRw11)
Synonyms:
Apo-2L, HLA class II histocompatibility antigen DRB1-11 beta chain, MHC class I antigen DRB1*11, DR-5, DRw11
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Rat (Rt)
Published Species Reactivity: Human (Hu)
Immunogen: Synthetic Peptide: C V(255) P E Q E M E V Q E P A E P T G(270)
Ordering Information
Pierce CD262 / TRAIL Receptor 2 DR5 Antibody
Product #
PA1-957
Size
100 µg
Price
$316.00
Purchase
Add CD262 / TRAIL Receptor 2 DR5 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1 µg/ml
Published Applications Dilution
Western Blot (WB) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-957 detects death receptor 5 (DR5) from human and rat tissues and cells.

PA1-957 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~58 kDa protein representing DR5 from HEK293 cell lysate.

PA1-957 immunizing peptide corresponds to amino acid residues 255-270 of rat DR5. PA1-957 immunizing peptide (Cat. # PEP-048) is available for use in neutralization and control experiments.
General Information
DR5 is a recently identified death domain containing receptor for TRAIL, which mediates TRAIL induced apoptosis. DR5 is also known as Apo2, TRAIL-R2, TRICK2 and KILLER.
Product Images
  • CD262 / TRAIL Receptor 2 DR5 Antibody (PA1-957) in WB
    PA1-957_WB.jpg

    Western Blot with anti-CD262  /  TRAIL Receptor 2 DR5 Polyclonal Antibody (PA1-957) (PA1-957_WB.jpg)

    Western Blot with anti-CD262 / TRAIL Receptor 2 DR5 Polyclonal Antibody (PA1-957)

    Western blot analysis of TRAIL Receptor 2 was performed by loading 25 ug of Hela (lane 1) and PC12 (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a TRAIL Receptor 2 polyclonal antibody (Product # PA1-957) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~58 kDa in Hela cells.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / Not Cited
PA1-957 was used in western blot to investigate the regulation of apoptosis induced by TRAIL

Science. 1997 Aug 8;277(5327):818-21.
"Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors."
Author(s): Sheridan JP, Marsters SA, Pitti RM, Gurney A, Skubatch M, Baldwin D, Ramakrishnan L, Gray CL, Baker K, Wood WI, Goddard AD, Godowski P, Ashkenazi A
Number of Citations: 135
(See PubMed article )
  Hu / 0
PA1-957 was used in western blot to investigate the role of BCL2L10 in cell cycle regulation in tumors

J Pathol. 2011 Feb;223(3):400-9.
"BCL2L10 protein regulates apoptosis/proliferation through differential pathways in gastric cancer cells."
Author(s): Xu JD, Cao XX, Long ZW, Liu XP, Furuya T, Xu JW, Liu XL, De Xu Z, Sasaki K, Li QQ
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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