CD25 / IL-2R alpha chain Antibody (2R12)

CD25 / IL-2R alpha chain Monoclonal Antibody for Western blot, IF, ICC, IHC, FACS, IP

>> See 50 other antibodies for IL2RA (IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; interleukin-2 receptor subunit alpha; p55)
Synonyms:
IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; interleukin-2 receptor subunit alpha; p55
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1, kappa
Class: Monoclonal
Type: Antibody
Clone: 2R12
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Human (Hu)
Immunogen: p55 alpha chain of the human IL-2 receptor
Ordering Information
Pierce CD25 / IL-2R alpha chain Antibody (2R12)
Product #
M10AR
Size
100 tests
Price
$250.00
Purchase
Add CD25 / IL-2R alpha chain Antibody (2R12) to your cart.
Tested Applications Dilution *
Western Blot (WB) Assay Dependent
Immunofluorescence (IF) 1:10-1:100
Immunocytochemistry (ICC) 1:10-1:100
Immunohistochemistry (IHC) Assay Dependent
Flow Cytometry (FACS) Assay Dependent
Immunoprecipitation (IP) Assay Dependent
Published Applications Dilution
Flow Cytometry (FACS) See publications below
Immunoprecipitation (IP) See publications below
Blocking Assay (BLOCK) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 20µg/ml
Purification: Protein G
Storage Buffer: PBS with 0.5% BSA
Preservative: 0.1% sodium azide
Storage Conditions: 4° C
Product Specific Information
M10AR targets CD25 in ICC/IF, FACS, IHC, IP, and WB applications and shows reactivity with Human samples.

The M10AR immunogen is p55 alpha chain of the human IL-2 receptor.

M10AR detects CD25 which has a predicted molecular weight of approximately 28 kDa.
General Information
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
Product Images
  • CD25 / IL-2R alpha chain Antibody (M10AR) in IF
    M10AR_IF_Jurkat_20131018115653.jpg

    Immunofluorescence with anti-CD25  /  IL-2R alpha chain Monoclonal Antibody [2R12] (M10AR) (M10AR_IF_Jurkat_20131018115653.jpg)

    Immunofluorescence with anti-CD25 / IL-2R alpha chain Monoclonal Antibody [2R12] (M10AR)

    Immunofluorescent analysis of CD25 (green) showing staining in the cytoplasm of Jurkat cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD25 monoclonal antibody (Product # M10AR) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • CD25 / IL-2R alpha chain Antibody (M10AR) in FACS
    M10AR_FACS_Jurkat.jpg

    Flow Cytometry with anti-CD25  /  IL-2R alpha chain Monoclonal Antibody [2R12] (M10AR) (M10AR_FACS_Jurkat.jpg)

    Flow Cytometry with anti-CD25 / IL-2R alpha chain Monoclonal Antibody [2R12] (M10AR)

    Flow cytometry analysis of CD25 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD25 monoclonal antibody (Product # M10AR) at a dilution of 1:10 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
Publications:
Flow Cytometry
  Species / Dilution Summary
  Hu / Not Cited
M10AR was used in flow cytometry to investigate the granular lymphocyte expansions with specific monoclonal antibodies

Blood. 1992 Oct 1;80(7):1765-73.
"Heterogeneous immunophenotype of granular lymphocyte expansions: differential expression of the CD8 alpha and CD8 beta chains."
Author(s): de Totero D, Tazzari PL, DiSanto JP, di Celle PF, Raspadori D, Conte R, Gobbi M, Ferrara GB, Flomenberg N, Lauria F
Number of Citations: 3
(See PubMed article )
  Hu / 1:100-1:200
M10AR was used in blocking/activating experiment, flow cytometry and immunoprecipitation to investigate the functional and structural properties of interleukin 2 receptors on activated B cells

J Immunol. 1985 Apr;134(4):2387-92.
"Structural and functional characterization of IL 2 receptors on activated human B cells."
Author(s): Boyd AW, Fisher DC, Fox DA, Schlossman SF, Nadler LM
Number of Citations: 9
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
M10AR was used in blocking/activating experiment, flow cytometry and immunoprecipitation to investigate the functional and structural properties of interleukin 2 receptors on activated B cells

J Immunol. 1985 Apr;134(4):2387-92.
"Structural and functional characterization of IL 2 receptors on activated human B cells."
Author(s): Boyd AW, Fisher DC, Fox DA, Schlossman SF, Nadler LM
Number of Citations: 9
(See PubMed article )
Blocking Assay
  Species / Dilution Summary
  Hu / 1:300
M10AR was used in blocking/activating experiment, flow cytometry and immunoprecipitation to investigate the functional and structural properties of interleukin 2 receptors on activated B cells

J Immunol. 1985 Apr;134(4):2387-92.
"Structural and functional characterization of IL 2 receptors on activated human B cells."
Author(s): Boyd AW, Fisher DC, Fox DA, Schlossman SF, Nadler LM
Number of Citations: 9
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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