* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
PBS, pH 7.4,
0.02% sodium azide
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
PA5-19612 targets VE Cadherin in flow cytometry, immunofluorescence, immunohistochemistry (paraffin), and Western blot applications and shows reactivity with Chicken, Human, and mouse samples. For IHC (P) perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
The PA5-19612 immunogen is synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human VE Cadherin.
PA5-19612 detects a band of approximately 115 kDa (predicted molecular weight 87 kDa).
This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined.
CD144/VE-Cadherin Antibody (PA5-19612) in WB PA5-19612_WesternBlot.jpg
Western Blot with anti-CD144/VE-Cadherin Polyclonal Antibody (PA5-19612)
Western blot analysis of Human Kidney Tissue Lysate using PA5-19612, VE Cadherin primary antibody at a dilution of 1 ug/ml. Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
CD144/VE-Cadherin Antibody (PA5-19612) in IF PA5-19612_Immunofluorescence.jpg
Immunofluorescence with anti-CD144/VE-Cadherin Polyclonal Antibody (PA5-19612)
Immunofluorescent staining of MCF-7 cells using PA5-19612, anti-VE Cadherin antibody. The cells were fixed with methanol (100%) for 5 minutes, permabilised with TBS-T (20mins), BSA(1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% PBS-Tween for 1 hour and exposed to the primary antibody at a concentration of 5 ug/ml overnight at 4C. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
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