CD107a / LAMP-1 Antibody

CD107a / LAMP-1 Polyclonal Antibody for Western blot, IF

>> See 3 other antibodies for LAMP1 (Lysosome-Associated Membrane Protein-1, LAMPA, LGP120)
Synonyms:
Lysosome-Associated Membrane Protein-1, LAMPA, LGP120
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Human (Hu)
Immunogen: Synthetic peptide corresponding to residues C K(407) R S H A G Y Q T I(416) of human LAMP1.
Ordering Information
Pierce CD107a / LAMP-1 Antibody
Product #
PA1-654A
Size
100 µg
Price
$360.00
Purchase
Add CD107a / LAMP-1 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:250 - 1:1000
Immunofluorescence (IF) Assay dependent
Published Applications Dilution
Immunocytochemistry (ICC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Affinity chromatography
Storage Buffer: 0.02M tris, pH 7.4, with 0.1M glycine
Preservative: 0.1% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-654A detects Lamp1 from human samples.

PA1-654A has been successfully used in immunofluorescence and Western blotting procedures. In immunofluorescence procedures, this antibody stains Lamp1 in HeLa cells resulting in perinuclear staining.

The PA1-654A immunogen is a synthetic peptide corresponding to residues C K(407) R S H A G Y Q T I(416) of human LAMP1. This region is completely conserved between human, mouse and rat.
General Information
Lysosomes are membrane-bound vacuoles which play a critical role in cellular metabolism. These organelles participate in endocytosis, the synthesis and assembly of acid hydrolases, act as sites for digestion of foreign materials and for specialized autolytic cellular processes. Membrane-associated glycoproteins have an important role in normal lysosomal function. Lamp1, also known as lysosome associated membrane protein 1, is a heavily glycosylated membrane protein of the lysosome. It contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail.
Product Images
  • CD107a / LAMP-1 Antibody (PA1-654A) in WB
    LAMP1_Antibody_PA1-654A_Western-Blot_1_20140313124851.jpg

    Western Blot with anti-CD107a  /  LAMP-1 Polyclonal Antibody (PA1-654A) (LAMP1_Antibody_PA1-654A_Western-Blot_1_20140313124851.jpg)

    Western Blot with anti-CD107a / LAMP-1 Polyclonal Antibody (PA1-654A)

    Western blot analysis of LAMP1 was performed by loading 20 µg of HeLa cell lysate and 5 µl of Molecular Weight Protein Ladder per well onto a 4-12% Bis-Tris polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an anti-LAMP1 polyclonal antibody (Product # PA1654A) at a dilution of 1:500 for one hour at room temperature, washed in TBS-0.1%Tween-20, and probed with a goat anti-rabbit IgG-HRP secondary antibody (Product # 32260) at a dilution of 1:100,000 for 1hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).
  • CD107a / LAMP-1 Antibody (PA1-654A) in IF
    LAMP1-Antibody-PA1-654A-Immunofluorescence-IF.jpg

    Immunofluorescence with anti-CD107a  /  LAMP-1 Polyclonal Antibody (PA1-654A) (LAMP1-Antibody-PA1-654A-Immunofluorescence-IF.jpg)

    Immunofluorescence with anti-CD107a / LAMP-1 Polyclonal Antibody (PA1-654A)

    Immunofluorescence staining of Lamp1 (red) in HeLa cells using PA1-654A.
Publications:
Immunocytochemistry
  Species / Dilution Summary
  Hu / Not Cited
PA1-654A was used in immunocytochemistry to investigate the role of VCP/p97 on the maturation of autophagosome

Autophagy. 2010 Feb;6(2):217-27.
"VCP/p97 is essential for maturation of ubiquitin-containing autophagosomes and this function is impaired by mutations that cause IBMPFD."
Author(s): Tresse E, Salomons FA, Vesa J, Bott LC, Kimonis V, Yao TP, Dantuma NP, Taylor JP
Number of Citations: 3
(See PubMed article )
  Hu / Not Cited
PA1-654A was used in immunocytochemistry to investigate the angiotensin type 1 receptor subcellular trafficking and degradation

J Biomed Opt. 2010 Sep-Oct;15(5):056003.
"Actin cytoskeleton-dependent Rab GTPase-regulated angiotensin type I receptor lysosomal degradation studied by fluorescence lifetime imaging microscopy."
Author(s): Li H, Yu P, Sun Y, Felder RA, Periasamy A, Jose PA
Number of Citations: 2
(See PubMed article )
  Hu / 0
PA1-654A was used in immunocytochemistry to investigate the effect of retromer malfunction on amyloidogenic APP processing

Neurobiol Dis. 2011 Aug;43(2):338-45.
"Retromer disruption promotes amyloidogenic APP processing."
Author(s): Sullivan CP, Jay AG, Stack EC, Pakaluk M, Wadlinger E, Fine RE, Wells JM, Morin PJ
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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