Part of Thermo Fisher Scientific

C5b-9 Monoclonal Antibody (aE11)

C5b-9 Antibody for ELISA, IHC

Synonyms:
CHE1, E1
Entrez Gene ID:
(Human) 590
UniProt ID:
P06276
Details
Host / Isotype: Mouse / IgG2a, kappa
Class: Monoclonal
Type: Antibody
Clone: aE11
Species Reactivity: Human (Hu) Non-human primate (Nhp) Porcine (Po) Equine (Eq)
Immunogen: Purified C5b-9.
Ordering Information
Pierce C5b-9 Monoclonal Antibody (aE11)
Product Number Pkg. Size Price Purchase
DIA 011-01-02 200 µg $542.00
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Storage: 4º C, store in dark
Form: 200 µg of protein A purified antibody (1 mg/ml) in 10 mM phosphate buffer pH 7.4 with 0.15 M NaCl and 0.09% sodium azide.


Applications Dilution *
Immunohistochemistry (IHC) Assay Dependent
ELISA (ELISA) 1/20,000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
DIA 011-01-02 binds both membrane-bound MAC and fluid-phase SC5b-9 complexes from human, porcine, baboon and equine samples.

DIA 011-01-02 has been successfully used in ELISA and IHC applications. This antibody is not recommended for Western blotting as the epitope is destroyed during the process.

The DIA 011-01-02 immunogen is purified C5b-9.
General Information
C5b-9 is also known as the TCC. TCC consists of C5b, C6, C7, C8 and C9. The complex forms transmembrane channels which displace lipid molecules and other constituents, thus disrupting the phospholipid bilayer of target cells leading to cell lysis by osmotic leakage. The complex contains neoantigens that are absent from the individual native components and the monoclonal antibody DIA 011-01 is directed against a neoepitope exposed in C9 when incorporated into TCC.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)