Bovine TNF alpha Recombinant Protein

Bovine TNF alpha Recombinant Protein for Western blot, IP, ELISA

>> See 58 other proteins for TNF (Bovine Tumor Necrosis Factor alpha TNFA)
Bovine Tumor Necrosis Factor alpha TNFA
Entrez Gene ID:
UniProt ID:
Host / Isotype: Yeast
Class: Recombinant
Type: Protein
Tested Species Reactivity: Bovine (Bv)
Ordering Information
Pierce Bovine TNF alpha Recombinant Protein
Product #
20 ug
Tested Applications Dilution *
Western Blot (WB) Assay Dependent
Immunoprecipitation (IP) Assay Dependent
ELISA (ELISA) Assay Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Lyophilized
Purification: purified
Storage Buffer: PBS
Preservative: no preservative
Storage Conditions: 4° C
Product Specific Information
For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C.

Activity: The biological activity of recombinant bovine TNFa was measured by its cytolytic effect on mouse WEHI-13VAR cells in the presence of Actinomycin D. The ED50 (or 1 unit of activity) for this effect in our laboratories typically falls into the range of 28.4 – 42.7 ng/ml.

Expression: The mature, soluble form of bovine TNF-alpha was expressed in Pichia pastoris, KM71H. GenBank® Accession # Q06599, amino acids 77 - 233. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: LRSSSQASSN.

Reconstitute with 0.5 ml of distilled water.
General Information
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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