Bovine IL-8 Recombinant Protein

Bovine IL-8 Recombinant Protein for Western blot, IP, ELISA

>> See 15 other proteins for CXCL8 (Bovine Interleukin 8 IL8)
Bovine Interleukin 8 IL8
Entrez Gene ID:
UniProt ID:
Host / Isotype: Yeast
Class: Recombinant
Type: Protein
Tested Species Reactivity: Bovine (Bv)
Ordering Information
Pierce Bovine IL-8 Recombinant Protein
Product #
20 ug
Tested Applications Dilution *
Western Blot (WB) Assay Dependent
Immunoprecipitation (IP) Assay Dependent
ELISA (ELISA) Assay Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Lyophilized
Purification: purified
Storage Buffer: PBS
Preservative: no preservative
Storage Conditions: 4° C
Product Specific Information
For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C.

Activity: The biological activity was determined by bovine neutrophil chemotactic response to bovine IL-8. The ED50 (or 1 unit of activity) for this effect in our laboratories is typically < 15.7% total responding cells at the concentration of Bovine IL-8 at 50 ng/mL.

Expression: The mature form of bovine IL-8 was expressed in Pichia pastoris, X-33. GenBank® Accession # P79255, amino acids 23-101. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: MSTELRCQCI.

Reconstitute with 0.5 ml of distilled water.
General Information
The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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