* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C.
Activity: The biological activity of recombinant bovine IL-4 was determined by measuring the proliferation of the factor-dependent human T-helper cell line, TF-1 (Kitamura, T. et al. (1989) J. Cell Physiol. 140:323). The ED50 (or 1 unit of activity) for this effect in our laboratories typically falls into the range of 0.44 – 0.67 ng/ml.
Expression: Mature bovine IL-4 was expressed in Pichia pastoris, X-33. GenBank® Accession # P30367, amino acids 25 - 135. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: HKCDITLAEI.
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
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