Bovine IL-1 beta Recombinant Protein

Bovine IL-1 beta Recombinant Protein for Western blot, IP, ELISA

>> See 18 other proteins for IL1B (Bovine Interleukin 1 beta IL1B)
Synonyms:
Bovine Interleukin 1 beta IL1B
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Yeast
Class: Recombinant
Type: Protein
Tested Species Reactivity: Bovine (Bv)
Ordering Information
Pierce Bovine IL-1 beta Recombinant Protein
Product #
RBOIL1BI
Size
20 ug
Price
$299.00
Purchase
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Tested Applications Dilution *
Western Blot (WB) Assay Dependent
Immunoprecipitation (IP) Assay Dependent
ELISA (ELISA) Assay Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Lyophilized
Purification: purified
Storage Buffer: PBS
Preservative: no preservative
Storage Conditions: 4° C
Product Specific Information
For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C.

Activity: The biological activity of recombinant bovine IL-1-beta was determined by measuring the proliferation of mouse D10.G4.1 cells (Kaye J. et al. (1984) J. Immunol. 133:1339). The ED50 (or 1 unit of activity) for this effect in our laboratories typically falls into the range of 14.1 – 21.1 pg/ml.

Expression: Mature bovine IL-1-beta was expressed in Pichia pastoris, KM71H. GenBank® Accession # P09428, amino acids 114 - 266. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: *EAEAAPVQSI. *The underlined sequence is due to incomplete cleavage of the alpha-factor secretory signal sequence by the Ste 13 protease.

Reconstitute with 0.5 ml of distilled water.
General Information
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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