* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C.
Activity: The biological activity of recombinant bovine IL-1-beta was determined by measuring the proliferation of mouse D10.G4.1 cells (Kaye J. et al. (1984) J. Immunol. 133:1339). The ED50 (or 1 unit of activity) for this effect in our laboratories typically falls into the range of 14.1 – 21.1 pg/ml.
Expression: Mature bovine IL-1-beta was expressed in Pichia pastoris, KM71H. GenBank® Accession # P09428, amino acids 114 - 266. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: *EAEAAPVQSI. *The underlined sequence is due to incomplete cleavage of the alpha-factor secretory signal sequence by the Ste 13 protease.
Reconstitute with 0.5 ml of distilled water.
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
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