Bovine IL-1 beta Recombinant Protein
Bovine IL-1 beta Recombinant Protein for ELISA, IP, Western blot
| Details | |
| Host / Isotype: | Yeast |
| Class: | Recombinant |
| Type: | Protein |
| Species Reactivity: | Bovine (Bv) |
| Ordering Information | ||||
| Pierce Bovine IL-1 beta Recombinant Protein |
| Storage: | 4° C |
| Form: | 20 ug of lyophilized recombinant protein from PBS, without preservatives or carrier proteins. Reconstitute with 0.5 ml of distilled or deionized water. |
| Applications | Dilution * |
| Western Blot (WB) | Assay Dependent |
| Immunoprecipitation (IP) | Assay Dependent |
| ELISA (ELISA) | Assay Dependent |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C. Activity: The biological activity of recombinant bovine IL-1-beta was determined by measuring the proliferation of mouse D10.G4.1 cells (Kaye J. et al. (1984) J. Immunol. 133:1339). The ED50 (or 1 unit of activity) for this effect in our laboratories typically falls into the range of 14.1 – 21.1 pg/ml. Expression: Mature bovine IL-1-beta was expressed in Pichia pastoris, KM71H. GenBank® Accession # P09428, amino acids 114 - 266. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: *EAEAAPVQSI. *The underlined sequence is due to incomplete cleavage of the alpha-factor secretory signal sequence by the Ste 13 protease. |
| General Information |
| The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. |
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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Bovine IL-1 beta Products > Bovine IL-1 beta Recombinant Protein RBOIL1BI
