* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
-80° C, Avoid Freeze/Thaw Cycles
Product Specific Information
For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS.
Activity: The biological activity of recombinant bovine IFN-gamma was determined in a viral challenge assay using VS virus on MDBK cells. Plates were stained with crystal violet, a visual CPE determination was performed and the dye was then solubilized and absorbance was read. Treatment of MDBK cells with 6 ng/ml of bovine IFN-gamma causes 25% Cytoprotection in the assay.
Expression: The mature form of bovine IFN-gamma was expressed in Pichia pastoris, X-33. GenBank® Accession # AAB64477, amino acids 24 – 166. Refs: Picha, K.S. and P.E. Baker. (1986) Immunol. 57:131. Moscovitch-Lopatin, M. et al. (1991) Eur. J. Immunol. 21:1439.
IFN gamma, also called Type II interferon, macrophage activation factor, and immune interferon is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE it appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFNg is involved in cytokine regulation and also acts synergistically with other cytokines.
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