Biotin Antibody (BTN.4)

Biotin Monoclonal Antibody for FACS, Western blot

Details

Host / Isotype:	Mouse / IgG1, kappa
Class:	Monoclonal
Type:	Antibody
Clone:	BTN.4
Species Reactivity:	All (All)
Immunogen:	Biotin conjugated to BSA

Ordering Information
Pierce Biotin Antibody (BTN.4)

Product #

MA5-11251

Size

500 ul

Price

$182.00

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Storage:	4° C, do not freeze
Form:	500 ul of antibody purified from the ascites fluid by Protein G chromatography (200 ug/ml). Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Applications	Dilution *

Western Blot (WB)	0.5 µg/ml
Flow Cytometry (FACS)	Assay dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Product Specific Information

MA5-11251 targets Biotin in Western blot and flow cytometry applications and shows reactivity with All samples.

The MA5-11251 immunogen is biotin conjugated to BSA.

General Information

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds.

PubMed References:

FACS (1)
WB (4)

Flow Cytometry
	Species / Dilution	Summary
	N/A / 1 ug/ml	MA5-11251 was used in flow cytometry and western blot to perform high-throughput antibody screening using detergent-solubilized and biotinylated whole-cell lysates as the antigen source Protein Eng Des Sel. 2010 Jul;23(7):567-77. "Antibody library screens using detergent-solubilized mammalian cell lysates as antigen sources." Author(s): Cho YK, Shusta EV Number of Citations: 1 (See PubMed article )

Western Blot
	Species / Dilution	Summary
	Ms / Not Cited	MA5-11251 was used in western blot to investigate a rapid method for generating bi-specific antibodies PLoS One. 2011;6(7):e22533. "A novel redox method for rapid production of functional bi-specific antibodies for use in early pilot studies." Author(s): Carlring J, De Leenheer E, Heath AW Number of Citations: 0 (See PubMed article )
	N/A / 0.5 ug/ml	MA5-11251 was used in western blot to develop a novel yeast display immunoprecipitation method for antigen isolation and characterization J Immunol Methods. 2009 Feb 28;341(1-2):117-26. "A yeast display immunoprecipitation method for efficient isolation and characterization of antigens." Author(s): Cho YK, Chen I, Wei X, Li L, Shusta EV Number of Citations: 3 (See PubMed article )
	N/A / 0.5 ug/ml	MA5-11251 was used in flow cytometry and western blot to perform high-throughput antibody screening using detergent-solubilized and biotinylated whole-cell lysates as the antigen source Protein Eng Des Sel. 2010 Jul;23(7):567-77. "Antibody library screens using detergent-solubilized mammalian cell lysates as antigen sources." Author(s): Cho YK, Shusta EV Number of Citations: 1 (See PubMed article )
	Rt / Not Cited	MA5-11251 was used in western blot to investigate a rapid method for generating bi-specific antibodies PLoS One. 2011;6(7):e22533. "A novel redox method for rapid production of functional bi-specific antibodies for use in early pilot studies." Author(s): Carlring J, De Leenheer E, Heath AW Number of Citations: 0 (See PubMed article )

(This product is for In Vitro experimental use only. Not for resale without express authorization.)