Beclin-1 Antibody

Beclin-1 Polyclonal Antibody for Western blot, IF, ICC, IHC (P), IP

>> See 12 other antibodies for BECN1 (CTIP-1 COUP-TF interacting protein 1)
Synonyms:
CTIP-1 COUP-TF interacting protein 1
Entrez Gene ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Immunogen: Synthetic peptide to human Beclin 1, containing residues within 1-100.
Ordering Information
Pierce Beclin-1 Antibody
Product #
PA1-16857
Size
100 µl
Price
$350.00
Purchase
Add Beclin-1 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500-1:2000
Immunofluorescence (IF) 1:50-1:200
Immunocytochemistry (ICC) 1:50-1:200
Immunohistochemistry (Paraffin) (IHC (P)) 1:400
Immunoprecipitation (IP) 1:80
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1.15mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS with 30% glycerol
Preservative: 0.05% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
The target sequence has 88% sequence homology with rat.

Suggested positive control: antigen standard for BECN1 (transient overexpression lysate).
General Information
Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biologic processes in which autophagy is important such as cell differentiation and nutritional stress responses.
Product Images
  • Beclin-1 Antibody (PA1-16857) in WB
    PA1-16857_Westernblot.jpg

    Western Blot with anti-Beclin-1 Polyclonal Antibody (PA1-16857) (PA1-16857_Westernblot.jpg)

    Western Blot with anti-Beclin-1 Polyclonal Antibody (PA1-16857)

    Western Blot detection of Beclin in mouse liver tissue lysate (50 ug) using PA1-16857. ECL Western Blot detection at 1 minute.
  • Beclin-1 Antibody (PA1-16857) in ICC
    PA1-16857_Immunoctyochemistry.jpg

    Immunocytochemistry with anti-Beclin-1 Polyclonal Antibody (PA1-16857) (PA1-16857_Immunoctyochemistry.jpg)

    Immunocytochemistry with anti-Beclin-1 Polyclonal Antibody (PA1-16857)

    Beclin 1 staining in Hela cells detected with a Dylight 488 labeled secondary antibody using PA1-16857.
  • Beclin-1 Antibody (PA1-16857) in IHC
    PA1-16857_Immunohistochemistry.jpg

    Immunohistochemistry with anti-Beclin-1 Polyclonal Antibody (PA1-16857) (PA1-16857_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-Beclin-1 Polyclonal Antibody (PA1-16857)

    Immunohistochemical staining of Pheochromocytes of the Adrenal Medulla tissue at 40x; using PA1-16857.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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