BLM Antibody

BLM Polyclonal Antibody for Western blot, IF, ICC, IHC (P)

>> See 9 other antibodies for BLM (BS, RECQ2, RECQL2, RECQL3, Bloom syndrome protein, DNA helicase, RecQ-like type 2, recQ protein-like 3)
Synonyms:
BS, RECQ2, RECQL2, RECQL3, Bloom syndrome protein, DNA helicase, RecQ-like type 2, recQ protein-like 3
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Recombinant fragment corresponding to a region within amino acids 1235 and 1417 of Human BLM
Ordering Information
Pierce BLM Antibody
Product #
PA5-27384
Size
100 µL
Price
$324.00
Purchase
Tested Applications Dilution *
Western Blot (WB) 1:500-1:3000
Immunofluorescence (IF) 1:100-1:1000
Immunocytochemistry (ICC) 1:100-1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 1:100-1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.35mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: 0.1M tris, pH 7, with 10% glycerol, 0.1M glycine
Preservative: 0.01% thimerosal
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-27384 targets BLM in IF, IHC (P), and WB applications and shows reactivity with Human samples.

The PA5-27384 immunogen is recombinant fragment corresponding to a region within amino acids 1235 and 1417 of Human BLM.
General Information
The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination.
Product Images
  • BLM Antibody (PA5-27384) in WB
    PA5-27384_WB_1.jpg

    Western Blot with anti-BLM Polyclonal Antibody (PA5-27384) (PA5-27384_WB_1.jpg)

    Western Blot with anti-BLM Polyclonal Antibody (PA5-27384)

    Western blot analysis of BLM using 30µg of A) Jurkat (B) Raji (C) K562 and D) NCI-H929 lysate. Samples were loaded onto a 5% SDS-PAGE  gel and probed with a BLM polyclonal antibody (Product # PA5-27384) at a dilution of 1:1000.
  • BLM Antibody (PA5-27384) in WB
    PA5-27384_WB_2.jpg

    Western Blot with anti-BLM Polyclonal Antibody (PA5-27384) (PA5-27384_WB_2.jpg)

    Western Blot with anti-BLM Polyclonal Antibody (PA5-27384)

    Western blot analysis of BLM using 30µg of A) NIH-3T3 (B) JC and C) BCL-1 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a BLM polyclonal antibody (Product # PA5-27384) at a dilution of 1:1000.
  • BLM Antibody (PA5-27384) in IF
    PA5-27384_IF.jpg

    Immunofluorescence with anti-BLM Polyclonal Antibody (PA5-27384) (PA5-27384_IF.jpg)

    Immunofluorescence with anti-BLM Polyclonal Antibody (PA5-27384)

    Immunofluorescent analysis of BLM in paraformaldehyde-fixed A431 cells using a BLM polyclonal antibody (Product # PA5-27384) at a 1:200 dilution.
  • BLM Antibody (PA5-27384) in IHC (P)
    PA5-27384_IHC.jpg

    Immunohistochemistry (Paraffin) with anti-BLM Polyclonal Antibody (PA5-27384) (PA5-27384_IHC.jpg)

    Immunohistochemistry (Paraffin) with anti-BLM Polyclonal Antibody (PA5-27384)

    Immunohistochemical analysis of BLM in paraffin-embedded Cal27 xenograft using a BLM polyclonal antibody (Product # PA5-27384) at a dilution of 1:100.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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