BCL-2 Antibody (8E12)

BCL-2 Monoclonal Antibody for IF, ICC, IHC, FACS

>> See 17 other antibodies for BCL2 (Bcl-2; BCL2)
Synonyms:
Bcl-2; BCL2
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 8E12
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Synthetic peptide corresponding to residues surrounding BCL-2, conjugated to KLH.
Ordering Information
Pierce BCL-2 Antibody (8E12)
Product #
MA5-15668
Size
100 ul
Price
$316.00
Purchase
Add BCL-2 Antibody (8E12) to your cart.
Tested Applications Dilution *
Immunofluorescence (IF) 1/200 - 1/1000
Immunocytochemistry (ICC) 1:200-1:1000
Immunohistochemistry (IHC) 1/200 - 1/1000
Flow Cytometry (FACS) 1/200 - 1/400
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: ascites
Preservative: 0.03% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
MA5-15668 targets BCL-2 in FACS, IF, and IHC applications and shows reactivity with Human and mouse samples.

The MA5-15668 immunogen is synthetic peptide corresponding to residues surrounding BCL-2, conjugated to KLH.

MA5-15668 detects BCL-2 which has a predicted molecular weight of approximately 26kDa.
General Information
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
Product Images
  • BCL-2 Antibody (MA5-15668) in IF
    BCL-2_Antibody_MA5-15668_Immunofluorescence1-1252013.jpg

    Immunofluorescence with anti-BCL-2 Monoclonal Antibody [8E12] (MA5-15668) (BCL-2_Antibody_MA5-15668_Immunofluorescence1-1252013.jpg)

    Immunofluorescence with anti-BCL-2 Monoclonal Antibody [8E12] (MA5-15668)

    Immunofluorescence analysis of NIH/3T3 cells using BCL-2 monoclonal antibody (Product # MA5-15668) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
  • BCL-2 Antibody (MA5-15668) in IHC
    BCL-2_Antibody_MA5-15668_Immunohistochemical1-1252013.jpg

    Immunohistochemistry with anti-BCL-2 Monoclonal Antibody [8E12] (MA5-15668) (BCL-2_Antibody_MA5-15668_Immunohistochemical1-1252013.jpg)

    Immunohistochemistry with anti-BCL-2 Monoclonal Antibody [8E12] (MA5-15668)

    Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and human brain tissues (right) using BCL-2 monoclonal antibody (Product # MA5-15668) followed with DAB staining.
  • BCL-2 Antibody (MA5-15668) in FACS
    BCL-2_Antibody_MA5-15668_FlowCytometry-1252013.jpg

    Flow Cytometry with anti-BCL-2 Monoclonal Antibody [8E12] (MA5-15668) (BCL-2_Antibody_MA5-15668_FlowCytometry-1252013.jpg)

    Flow Cytometry with anti-BCL-2 Monoclonal Antibody [8E12] (MA5-15668)

    Flow cytometric analysis of 3T3L1 cells using BCL-2 monoclonal antibody (Product # MA5-15668) (green) and negative control (purple).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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