Part of Thermo Fisher Scientific

Avian reovirus p17 Polyclonal Antibody

Avian reovirus p17 Antibody for ELISA, Western blot

Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Species Reactivity: Virus (Vs)
Immunogen: Synthetic peptide of p17 (a.a.100-140).
Ordering Information
Pierce Avian reovirus p17 Polyclonal Antibody
Product Number Pkg. Size Price Purchase
GB-10379 100 µg $344.00
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This product is not available outside of the U.S.

Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: 100 µg of affinity purified antibody in PBS in lyophilized powder. Redissolve with 100 µl sterile water.


Applications Dilution *
ELISA (ELISA) 1:50,000
Western Blot (WB) Assay dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PG-10379 detects Avian retrovirus p17.

GB-10379 has been successfully used in ELISA and Western blot procedures.

The GB-10379 immunogen is a synthetic peptide of p10.

Store at 4ºC short term. For long term storage, store at -20ºC.
General Information
The second open reading frame of avian reovirus S1 gene segment encodes a 17 kDa non-structural protein, named p17. The biological role of p17 is fully unknown so far. Using trypan blue dye exclusion and MTTassay, demonstrated that the ectopic expression of p17 results in the reduction of viable cell number and cell proliferation rate of Vero, BHK, 293, and HeLa cells. Measurement of LDH activity and DNA fragmentation analysis revealed that p17 expression did not cause cell death or apoptosis. These data indicated that the p17 possessed the growth retardation function. Semi-quantitative RT-PCR and Western blotting revealed that p17-expressing cells induced the expression of CDK inhibitor p21cip1/waf1 in a time- and dose-dependen tmanner, but the transcripts of CDK inhibitor p15INK4b, p16INK4a, or p27kip were not altered.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)