Ataxin 7 Antibody

Ataxin 7 Polyclonal Antibody for Western blot, IHC (F)

Synonyms:
ADCAII, OPCA3, SCA7
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Drosophila (Dm)
Published Species Reactivity: Drosophila (Dm), Human (Hu), Mouse (Ms), Rat (Rt)
Immunogen: Synthetic Peptide: M(1) S E R A A D D V R G E P R R A A(17) C
Ordering Information
Pierce Ataxin 7 Antibody
Product #
PA1-749
Size
100 µg
Price
$316.00
Purchase
Add Ataxin 7 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1 ug/ml
Immunohistochemistry (Frozen) (IHC (F)) Assay dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-749 detects ataxin 7 protein from transfected cell lysate.

PA1-749 has successfully been used in immunohistochemistry and Western blot procedures. By Western blot, this antibody detects an ~96 kDa protein representing human ataxin 7 in transfected COS-1 cells.

The PA1-749 immunizing peptide corresponds to amino acid residues 1-17 from human ataxin 7. This sequence is completely conserved in mouse ataxin 7. This peptide (Cat. # PEP-217) is available for use in neutralization and control experiments.

General Information
Spinocerebellar ataxia type 7 (SCA7) belongs to a class of hereditary neurodegenrative diseases characterized by abnormally expanded polyglutamine (CAG) repeats at the N-terminus. This autosomal dominant cerebellar ataxia primarily affects the cerebellum, retina, and brain stem and causes dementia, macular degeneration and other neurodegenerative characteristics.

SCA7 gene encodes the ubiquitous 892 residue protein ataxin-7. This protein is typically located in the cytoplasm and on the nuclear membrane of normal brain neurons. In cells where there is a mutation of the SCA7 gene, ataxin 7 accumulates in intranuclear inclusions and can result in cell death.
Product Images
  • Ataxin 7 Antibody (PA1-749) in WB
    PA1-749_1_07232010.jpg

    Western Blot with anti-Ataxin 7 Polyclonal Antibody (PA1-749) (PA1-749_1_07232010.jpg)

    Western Blot with anti-Ataxin 7 Polyclonal Antibody (PA1-749)

    Western blot of ataxin 7 in transfected COS-1 cell lysate using PA1-749.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / 1:1,000
PA1-749 was used in western blot to study the relationship between ataxin-7 and caspase-7 and its role on SCA7 disease pathogenesis.

J Biol Chem. 2007 Oct 12;282(41):30150-60.
"Proteolytic cleavage of ataxin-7 by caspase-7 modulates cellular toxicity and transcriptional dysregulation."
Author(s): Young JE, Gouw L, Propp S, Sopher BL, Taylor J, Lin A, Hermel E, Logvinova A, Chen SF, Chen S, Bredesen DE, Truant R, Ptacek LJ, La Spada AR, Ellerby LM
Number of Citations: 1
(See PubMed article )
  Hu / 1:1,000
PA1-749 was used in western blot and immunoprecipitation to study the effect of ataxin-7 post-translational modification on its autophagy-mediated proteolytic processing.

J Neurosci. 2009 Dec 2;29(48):15134-44.
"Posttranslational modification of ataxin-7 at lysine 257 prevents autophagy-mediated turnover of an N-terminal caspase-7 cleavage fragment."
Author(s): Mookerjee S, Papanikolaou T, Guyenet SJ, Sampath V, Lin A, Vitelli C, DeGiacomo F, Sopher BL, Chen SF, La Spada AR, Ellerby LM
Number of Citations: 1
(See PubMed article )
  Hu / 1:1000
PA1-749 was used in immunohistochemistry and western blot to study the ability of IFNbeta to induce PML-dependent ataxin 7 clearance and improve motor function in a murine model of spinocerebellar ataxia 7

Brain. 2013 Jun;136(Pt 6):1732-45.
"Interferon ? induces clearance of mutant ataxin 7 and improves locomotion in SCA7 knock-in mice."
Author(s): Chort A, Alves S, Marinello M, Dufresnois B, Dornbierer JG, Tesson C, Latouche M, Baker DP, Barkats M, El Hachimi KH, Ruberg M, Janer A, Stevanin G, Brice A, Sittler A
Number of Citations: 2
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Rt / 1:500
PA1-749 was used in immunocytochemistry to study the pathogenesis of spinocerebellar ataxia type 7.

J Cell Biol. 2006 Jul 3;174(1):65-76.
"PML clastosomes prevent nuclear accumulation of mutant ataxin-7 and other polyglutamine proteins."
Author(s): Janer A, Martin E, Muriel MP, Latouche M, Fujigasaki H, Ruberg M, Brice A, Trottier Y, Sittler A
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Dm / 1:1,000
PA1-749 was used in immunohistochemistry to investigate a conditional Drosophila model for spinocerebellar ataxia 7 .

J Neurosci. 2007 Mar 7;27(10):2483-92.
"A conditional pan-neuronal Drosophila model of spinocerebellar ataxia 7 with a reversible adult phenotype suitable for identifying modifier genes."
Author(s): Latouche M, Lasbleiz C, Martin E, Monnier V, Debeir T, Mouatt-Prigent A, Muriel MP, Morel L, Ruberg M, Brice A, Stevanin G, Tricoire H
Number of Citations: 1
(See PubMed article )
  Ms / 1:500
PA1-749 was used in immunohistochemistry to investigate the role of ataxin-7 in spinocerebellar ataxia type 7 cerebellar disease

J Neurosci. 2011 Nov 9;31(45):16269-78.
"Spinocerebellar ataxia type 7 cerebellar disease requires the coordinated action of mutant ataxin-7 in neurons and glia, and displays non-cell-autonomous bergmann glia degeneration."
Author(s): Furrer SA, Mohanachandran MS, Waldherr SM, Chang C, Damian VA, Sopher BL, Garden GA, La Spada AR
Number of Citations: 1
(See PubMed article )
  Ms / 1:500
PA1-749 was used in immunohistochemistry to study whether spinocerebellar ataxia type 7 neurological features and neurodegeneration are reversible in a conditional murine model

Hum Mol Genet. 2013 Mar 1;22(5):890-903.
"Reduction of mutant ataxin-7 expression restores motor function and prevents cerebellar synaptic reorganization in a conditional mouse model of SCA7."
Author(s): Furrer SA, Waldherr SM, Mohanachandran MS, Baughn TD, Nguyen KT, Sopher BL, Damian VA, Garden GA, La Spada AR
Number of Citations: 3
(See PubMed article )
  Ms / 1:1000
PA1-749 was used in immunohistochemistry and western blot to study the ability of IFNbeta to induce PML-dependent ataxin 7 clearance and improve motor function in a murine model of spinocerebellar ataxia 7

Brain. 2013 Jun;136(Pt 6):1732-45.
"Interferon ? induces clearance of mutant ataxin 7 and improves locomotion in SCA7 knock-in mice."
Author(s): Chort A, Alves S, Marinello M, Dufresnois B, Dornbierer JG, Tesson C, Latouche M, Baker DP, Barkats M, El Hachimi KH, Ruberg M, Janer A, Stevanin G, Brice A, Sittler A
Number of Citations: 2
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
PA1-749 was used in western blot and immunoprecipitation to study the effect of ataxin-7 post-translational modification on its autophagy-mediated proteolytic processing.

J Neurosci. 2009 Dec 2;29(48):15134-44.
"Posttranslational modification of ataxin-7 at lysine 257 prevents autophagy-mediated turnover of an N-terminal caspase-7 cleavage fragment."
Author(s): Mookerjee S, Papanikolaou T, Guyenet SJ, Sampath V, Lin A, Vitelli C, DeGiacomo F, Sopher BL, Chen SF, La Spada AR, Ellerby LM
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific