Aryl Hydrocarbon Receptor Antibody (RPT1)

Aryl Hydrocarbon Receptor Monoclonal Antibody for Western blot, IF, IHC, IHC (P), IP

>> See 10 other antibodies for AHR (Ah receptor, AhR, Class E basic helix-loop-helix protein 76, bHLHe76, AHR, BHLHE76)
Synonyms:
Ah receptor, AhR, Class E basic helix-loop-helix protein 76, bHLHe76, AHR, BHLHE76
Entrez Gene ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: RPT1
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Published Species Reactivity: Human (Hu), Mouse (Ms), Non-human primate (Nhp), Porcine (Po), Rat (Rt)
Immunogen: Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of mouse AhR.
Ordering Information
Pierce Aryl Hydrocarbon Receptor Antibody (RPT1)
Product #
MA1-514
Size
100 µl
Price
$365.00
Purchase
Add Aryl Hydrocarbon Receptor Antibody (RPT1) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500 - 1:2000
Immunofluorescence (IF) 1:100 - 1:1000
Immunohistochemistry (IHC) 1:10-1:100
Immunohistochemistry (Paraffin) (IHC (P)) 1:10 - 1:100
Immunoprecipitation (IP) Assay dependent
Published Applications Dilution
Western Blot (WB) See publications below
ChIP assay (ChIP) See publications below
ELISA (ELISA) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: ascites
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA1-514 detects the aryl hydrocarbon receptor (AhR) from human, mouse, and rat cells.

MA1-514 has been successfully used in Western blotting, immunoprecipitation, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects an ~95 kDa protein representing AhR from Hepa 1 cytosol. Although MA1-514 can be used in immunoprecipitation procedures, MA1-513 (clone RPT9) is recommended for this procedure.

MA1-514 immunogen is residues 12-31 from the mouse AhR protein with amino acids 18-21 being omitted.
General Information
The aryl hydrocarbon receptor (AhR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AhR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the Ah Receptor.
 
Studies indicate that in non-ligand activated cells, AhR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). The AhR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.
Product Images
  • Aryl Hydrocarbon Receptor Antibody (MA1-514) in WB
    Aryl-Hydrocarbon-Receptor-Monoclonal-Antibody-RPT1-MA1-514-Western-Blot.jpg

    Western Blot with anti-Aryl Hydrocarbon Receptor Monoclonal Antibody [RPT1] (MA1-514) (Aryl-Hydrocarbon-Receptor-Monoclonal-Antibody-RPT1-MA1-514-Western-Blot.jpg)

    Western Blot with anti-Aryl Hydrocarbon Receptor Monoclonal Antibody [RPT1] (MA1-514)

    Western blot analysis of Aryl Hydrocarbon Receptor was performed by loading 40ug of HEK293 lysate overexpressing Aryl Hydrocarbon Receptor (right lane) or empty vector control (left lane) and 10ul of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an Aryl Hydrocarbon Receptor monoclonal antibody (Product # MA1-514) at a dilution of 1:1000 overnight at 4ºC on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34087).
  • Aryl Hydrocarbon Receptor Antibody (MA1-514) in IF
    Aryl-Hydrocarbon-Receptor_MA1-514_Antibody_Immunofluorescence_Hela.jpg

    Immunofluorescence with anti-Aryl Hydrocarbon Receptor Monoclonal Antibody [RPT1] (MA1-514) (Aryl-Hydrocarbon-Receptor_MA1-514_Antibody_Immunofluorescence_Hela.jpg)

    Immunofluorescence with anti-Aryl Hydrocarbon Receptor Monoclonal Antibody [RPT1] (MA1-514)

    Immunofluorescent analysis of Aryl Hydrocarbon Receptor in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Aryl Hydrocarbon Receptor monoclonal antibody (Product # MA1-514) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Aryl Hydrocarbon Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
  • Aryl Hydrocarbon Receptor Antibody (MA1-514) in IF
    Aryl-Hydrocarbon-Receptor_MA1-514_Antibody_Immunofluorescence_MCF-7.jpg

    Immunofluorescence with anti-Aryl Hydrocarbon Receptor Monoclonal Antibody [RPT1] (MA1-514) (Aryl-Hydrocarbon-Receptor_MA1-514_Antibody_Immunofluorescence_MCF-7.jpg)

    Immunofluorescence with anti-Aryl Hydrocarbon Receptor Monoclonal Antibody [RPT1] (MA1-514)

    Immunofluorescent analysis of Aryl Hydrocarbon Receptor in MCF-7 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Aryl Hydrocarbon Receptor monoclonal antibody (Product # MA1-514) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Aryl Hydrocarbon Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
  • Aryl Hydrocarbon Receptor Antibody (MA1-514) in IF
    Aryl-Hydrocarbon-Receptor_MA1-514_Antibody_Immunofluorescence_NIH-3T3.jpg

    Immunofluorescence with anti-Aryl Hydrocarbon Receptor Monoclonal Antibody [RPT1] (MA1-514) (Aryl-Hydrocarbon-Receptor_MA1-514_Antibody_Immunofluorescence_NIH-3T3.jpg)

    Immunofluorescence with anti-Aryl Hydrocarbon Receptor Monoclonal Antibody [RPT1] (MA1-514)

    Immunofluorescent analysis of Aryl Hydrocarbon Receptor in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Aryl Hydrocarbon Receptor monoclonal antibody (Product # MA1-514) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Aryl Hydrocarbon Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
  • Aryl Hydrocarbon Receptor Antibody (MA1-514) in IHC
    Aryl-Hydrocarbon-Receptor_MA1-514_Immunohistochemistry_Human-Breast-carcinoma.jpg

    Immunohistochemistry with anti-Aryl Hydrocarbon Receptor Monoclonal Antibody [RPT1] (MA1-514) (Aryl-Hydrocarbon-Receptor_MA1-514_Immunohistochemistry_Human-Breast-carcinoma.jpg)

    Immunohistochemistry with anti-Aryl Hydrocarbon Receptor Monoclonal Antibody [RPT1] (MA1-514)

    Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Aryl Hydrocarbon Receptor monoclonal antibody (Product # MA1-514) at a dilution of 1:100 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Aryl Hydrocarbon Receptor Antibody (MA1-514) in IHC
    Aryl-Hydrocarbon-Receptor_MA1-514_Immunohistochemistry_Human-Skeletal-muscle.jpg

    Immunohistochemistry with anti-Aryl Hydrocarbon Receptor Monoclonal Antibody [RPT1] (MA1-514) (Aryl-Hydrocarbon-Receptor_MA1-514_Immunohistochemistry_Human-Skeletal-muscle.jpg)

    Immunohistochemistry with anti-Aryl Hydrocarbon Receptor Monoclonal Antibody [RPT1] (MA1-514)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Aryl Hydrocarbon Receptor monoclonal antibody (Product # MA1-514) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / 1:1,000
MA1-514 was used in western blot to study how HES-1 is regulated by estrogen receptor and AhR

Mol Pharmacol. 2004 Jan;65(1):165-71.
"HES-1, a novel target gene for the aryl hydrocarbon receptor."
Author(s): Thomsen JS, Kietz S, Ström A, Gustafsson JA
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA1-514 was used in western blot to study the mechanism of TCDD-induced immortalization of primary keratinocytes.

J Biol Chem. 2004 Jun 25;279(26):27187-93.
"Dioxin-induced immortalization of normal human keratinocytes and silencing of p53 and p16INK4a."
Author(s): Ray SS, Swanson HI
Number of Citations: 1
(See PubMed article )
  Hu / 1:1,000
MA1-514 was used in western blot to investigate the change of CYP1-4 gene expression in differentiating epidermal cells exposed to drug compounds related to clinical dermatology practice or foreign compounds affecting normal epidermal differentiation

J Pharmacol Exp Ther. 2006 Dec;319(3):1162-71.
"Differentiation-specific factors modulate epidermal CYP1-4 gene expression in human skin in response to retinoic acid and classic aryl hydrocarbon receptor ligands."
Author(s): Du L, Neis MM, Ladd PA, Keeney DS
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA1-514 was used in western blot to study the effect of omeprazole on IGFBP-1-dependent physiological processes.

J Pharmacol Exp Ther. 2008 Mar;324(3):1102-10.
"Omeprazole stimulates the induction of human insulin-like growth factor binding protein-1 through aryl hydrocarbon receptor activation."
Author(s): Murray IA, Perdew GH
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA1-514 was used in western blot to investigate the effect of 6,2',4'-trimethoxyflavone for aryl hydrocarbon receptor signaling activity

J Pharmacol Exp Ther. 2010 Jan;332(1):135-44.
"Antagonism of aryl hydrocarbon receptor signaling by 6,2',4'-trimethoxyflavone."
Author(s): Murray IA, Flaveny CA, DiNatale BC, Chairo CR, Schroeder JC, Kusnadi A, Perdew GH
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA1-514 was used in western blot to study the regulation of Aryl hydrocarbon receptor activity and its anti-inflammatory therapeutic potential

Mol Pharmacol. 2010 Feb;77(2):247-54.
"Evidence for ligand-mediated selective modulation of aryl hydrocarbon receptor activity."
Author(s): Murray IA, Morales JL, Flaveny CA, Dinatale BC, Chiaro C, Gowdahalli K, Amin S, Perdew GH
Number of Citations: 13
(See PubMed article )
  Hu / 1:250
MA1-514 was used in western blot to study the effects of maternal smoking and fetal sex on metabolic enzyme expression in the fetal liver

J Clin Endocrinol Metab. 2011 Sep;96(9):2851-60.
"Maternal smoking and fetal sex significantly affect metabolic enzyme expression in the human fetal liver."
Author(s): O'Shaughnessy PJ, Monteiro A, Bhattacharya S, Fowler PA
Number of Citations: 0
(See PubMed article )
  Hu / 1:2000
MA1-514 was used in western blot to study the suppression of mammosphere formation in MCF-7 cells by aryl hydrocarbon receptor activation

Cancer Lett. 2012 Apr 28;317(2):192-8.
"Activation of the aryl hydrocarbon receptor represses mammosphere formation in MCF-7 cells."
Author(s): Zhao S, Kanno Y, Nakayama M, Makimura M, Ohara S, Inouye Y
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA1-514 was used in western blot to study the role of the aryl hydrocarbon receptor in controling hepatic fatty acid synthesis

Toxicol Sci. 2012 Oct;129(2):372-9.
"Role of the Ah receptor in homeostatic control of fatty acid synthesis in the liver."
Author(s): Tanos R, Murray IA, Smith PB, Patterson A, Perdew GH
Number of Citations: 0
(See PubMed article )
  Hu / 1:2000
MA1-514 was used in western blot to study the role of HER2-aryl hydrocarbon receptor interactions in mammosphere formation in MCF-7 breast cancer cells

Cancer Lett. 2013 Mar 1;330(1):41-8.
"HER2 overexpression-mediated inflammatory signaling enhances mammosphere formation through up-regulation of aryl hydrocarbon receptor transcription."
Author(s): Zhao S, Ohara S, Kanno Y, Midorikawa Y, Nakayama M, Makimura M, Park Y, Inouye Y
Number of Citations: 0
(See PubMed article )
  Ms / 1:10,000
MA1-514 was used in western blot to identify the subunits of AhR complex

J Biol Chem. 1994 Nov 4;269(44):27554-8.
"Subunit composition of the heteromeric cytosolic aryl hydrocarbon receptor complex."
Author(s): Chen HS, Perdew GH
Number of Citations: 16
(See PubMed article )
  Ms / Not Cited
MA1-514 was used in western blot to investigate the regulation of AhR by serum and growth factors in the cell cycle

J Biol Chem. 1996 Oct 18;271(42):25921-7.
"Expression of the aryl hydrocarbon receptor is regulated by serum and mitogenic growth factors in murine 3T3 fibroblasts."
Author(s): Vaziri C, Schneider A, Sherr DH, Faller DV
Number of Citations: 0
(See PubMed article )
  Ms / Not Cited
MA1-514 was used in western blot to investigate the function of XAP2 for the regulation of AhR stabilization

Biochemistry. 1999 Jul 13;38(28):8907-17.
"Characterization of the AhR-hsp90-XAP2 core complex and the role of the immunophilin-related protein XAP2 in AhR stabilization."
Author(s): Meyer BK, Perdew GH
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA1-514 was used in western blot to characterize mouse vs. human divergence in receptor-regulated gene expression

Toxicol Sci. 2010 Apr;114(2):217-25.
"Differential gene regulation by the human and mouse aryl hydrocarbon receptor."
Author(s): Flaveny CA, Murray IA, Perdew GH
Number of Citations: 2
(See PubMed article )
  Ms / 1:1,000
MA1-514 was used in western blot to investigate the mechanisms by which flavonoids suppress the AhR-mediated signal transduction in mouse hepatoma Hepa-1c1c7 cells

Arch Biochem Biophys. 2010 Sep 1;501(1):134-41.
"Suppression mechanisms of flavonoids on aryl hydrocarbon receptor-mediated signal transduction."
Author(s): Mukai R, Shirai Y, Saito N, Fukuda I, Nishiumi S, Yoshida K, Ashida H
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA1-514 was used in western blot to investigate the role of aryl hydrocarbon receptor in cholesterol biosynthetic pathway

Hepatology. 2012 Jun;55(6):1994-2004.
"Aryl hydrocarbon receptor regulates the cholesterol biosynthetic pathway in a dioxin response element-independent manner."
Author(s): Tanos R, Patel RD, Murray IA, Smith PB, Patterson AD, Perdew GH
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA1-514 was used in western blot to study the role of the aryl hydrocarbon receptor in controling hepatic fatty acid synthesis

Toxicol Sci. 2012 Oct;129(2):372-9.
"Role of the Ah receptor in homeostatic control of fatty acid synthesis in the liver."
Author(s): Tanos R, Murray IA, Smith PB, Patterson A, Perdew GH
Number of Citations: 0
(See PubMed article )
  Nhp / Not Cited
MA1-514 was used in western blot to indentify the phosphorylation status of the hepatitis B virus X-associated protein 2

Arch Biochem Biophys. 2002 Oct 15;406(2):209-21.
"Characterization of the phosphorylation status of the hepatitis B virus X-associated protein 2."
Author(s): Dull AB, Carlson DB, Petrulis JR, Perdew GH
Number of Citations: 1
(See PubMed article )
  Po / 1:250
MA1-514 was used in western blot to investigate the effects of TCDD and dioxin-like PCB 127 on the AhR signaling pathway and on the gene expression profiles of key factors related to thyroid function

Toxicol Sci. 2006 Feb;89(2):408-14.
"AhR-agonist-induced transcriptional changes of genes involved in thyroid function in primary porcine thyrocytes."
Author(s): Pocar P, Klonisch T, Brandsch C, Eder K, Fröhlich C, Hoang-Vu C, Hombach-Klonisch S
Number of Citations: 1
(See PubMed article )
  Po / 1:250
MA1-514 was used in western blot to study the impact of PHAHs on oviduct epithelium treated with dioxin-type endocrine disruptors

Toxicol Sci. 2006 Apr;90(2):519-28.
"Dioxin exerts anti-estrogenic actions in a novel dioxin-responsive telomerase-immortalized epithelial cell line of the porcine oviduct (TERT-OPEC)."
Author(s): Hombach-Klonisch S, Pocar P, Kauffold J, Klonisch T
Number of Citations: 1
(See PubMed article )
  Rt / 1:1500
MA1-514 was used in ELISA and western blot to investigate the effect of cacao polyphenol extract on transformation of an aryl hydrocarbon receptor in C57BL/6 mice

J Agric Food Chem. 2008 Nov 12;56(21):10399-405.
"Cacao polyphenol extract suppresses transformation of an aryl hydrocarbon receptor in C57BL/6 mice."
Author(s): Mukai R, Fukuda I, Nishiumi S, Natsume M, Osakabe N, Yoshida K, Ashida H
Number of Citations: 1
(See PubMed article )
ChIP assay
  Species / Dilution Summary
  Ms / Not Cited
MA1-514 was used in ChIP assay to investigate the role of genome-wide B1 retrotransposon on regulates gene expression in vivo

Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1632-7.
"Genome-wide B1 retrotransposon binds the transcription factors dioxin receptor and Slug and regulates gene expression in vivo."
Author(s): Roman AC, Benitez DA, Carvajal-Gonzalez JM, Fernandez-Salguero PM
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA1-514 was used in ChIP assay to investigate the role of dioxin receptor in the insulator activity of B1-X35S retrotransposon

Genome Res. 2011 Mar;21(3):422-32.
"Dioxin receptor and SLUG transcription factors regulate the insulator activity of B1 SINE retrotransposons via an RNA polymerase switch."
Author(s): Román AC, González-Rico FJ, Moltó E, Hernando H, Neto A, Vicente-Garcia C, Ballestar E, Gómez-Skarmeta JL, Vavrova-Anderson J, White RJ, Montoliu L, Fernández-Salguero PM
Number of Citations: 1
(See PubMed article )
ELISA
  Species / Dilution Summary
  Hu / Not Cited
MA1-514 was used in ELISA to evaluate a novel ELISA strategy for the screening of aryl hydrocarbon receptor activators and inhibitors

J Immunol Methods. 2004 Apr;287(1-2):187-201.
"A new southwestern chemistry-based ELISA for detection of aryl hydrocarbon receptor transformation: application to the screening of its receptor agonists and antagonists."
Author(s): Fukuda I, Nishiumi S, Yabushita Y, Mukai R, Kodoi R, Hashizume K, Mizuno M, Hatanaka Y, Ashida H
Number of Citations: 0
(See PubMed article )
  Ms / Not Cited
MA1-514 was used in ELISA to investigate the role of P-glycoprotein for supression on transformation of the aryl hydrocarbon receptor by kaempferol

Biosci Biotechnol Biochem. 2009 Jul;73(7):1635-9.
"Inhibition of P-glycoprotein enhances the suppressive effect of kaempferol on transformation of the aryl hydrocarbon receptor."
Author(s): Mukai R, Satsu H, Shimizu M, Ashida H
Number of Citations: 0
(See PubMed article )
  Rt / 0
MA1-514 was used in ELISA and western blot to investigate the effect of cacao polyphenol extract on transformation of an aryl hydrocarbon receptor in C57BL/6 mice

J Agric Food Chem. 2008 Nov 12;56(21):10399-405.
"Cacao polyphenol extract suppresses transformation of an aryl hydrocarbon receptor in C57BL/6 mice."
Author(s): Mukai R, Fukuda I, Nishiumi S, Natsume M, Osakabe N, Yoshida K, Ashida H
Number of Citations: 1
(See PubMed article )
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