ApoE Antibody (1H4)

ApoE Monoclonal Antibody for Western blot, IHC, FACS

>> See 10 other antibodies for APOE (AD2, LDLCQ5, LPG, apo-E; apolipoprotein E3)
Synonyms:
AD2, LDLCQ5, LPG, apo-E; apolipoprotein E3
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 1H4
Tested Species Reactivity: Human (Hu)
Immunogen: Purified recombinant fragment of human ApoE expressed in E. Coli.
Ordering Information
Pierce ApoE Antibody (1H4)
Product #
MA5-15852
Size
100 ul
Price
$316.00
Purchase
Add ApoE Antibody (1H4) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1/500 - 1/2000
Immunohistochemistry (IHC) 1/200 - 1/1000
Flow Cytometry (FACS) 1/200 - 1/400
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: ascites
Preservative: 0.03% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
MA5-15852 targets ApoE in FACS, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15852 immunogen is purified recombinant fragment of human ApoE expressed in E. Coli. .

MA5-15852 detects ApoE which has a predicted molecular weight of approximately 36kDa.
General Information
Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Tissue specificity: Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.
Product Images
  • ApoE Antibody (MA5-15852) in WB
    ApoE_Antibody_MA5-15852_Westernblot1-1252013.jpg

    Western Blot with anti-ApoE Monoclonal Antibody [1H4] (MA5-15852) (ApoE_Antibody_MA5-15852_Westernblot1-1252013.jpg)

    Western Blot with anti-ApoE Monoclonal Antibody [1H4] (MA5-15852)

    Western blot analysis of ApoE using ApoE monoclonal antibody (Product # MA5-15852) in HEK293 (1) and ApoE (AA: 20-267) human IgG Fc transfected HEK293 (2) cell lysate.
  • ApoE Antibody (MA5-15852) in IHC
    ApoE_Antibody_MA5-15852_Immunohistochemical1-1252013.jpg

    Immunohistochemistry with anti-ApoE Monoclonal Antibody [1H4] (MA5-15852) (ApoE_Antibody_MA5-15852_Immunohistochemical1-1252013.jpg)

    Immunohistochemistry with anti-ApoE Monoclonal Antibody [1H4] (MA5-15852)

    Immunohistochemical analysis of paraffin-embedded liver cancer tissues (left) and brain tissues (right) using ApoE monoclonal antibody (Product # MA5-15852) followed with DAB staining.
  • ApoE Antibody (MA5-15852) in FACS
    ApoE_Antibody_MA5-15852_FlowCytometry-1252013.jpg

    Flow Cytometry with anti-ApoE Monoclonal Antibody [1H4] (MA5-15852) (ApoE_Antibody_MA5-15852_FlowCytometry-1252013.jpg)

    Flow Cytometry with anti-ApoE Monoclonal Antibody [1H4] (MA5-15852)

    Flow cytometric analysis of HepG2 cells using ApoE monoclonal antibody (Product # MA5-15852) (green) and negative control (purple).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific