Angiostatin Antibody

Angiostatin Polyclonal Antibody for Western blot, IF, ICC

>> See 22 other antibodies for PLG (Plasminogen, Microplasmin, PLG)
Synonyms:
Plasminogen, Microplasmin, PLG
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Synthetic Peptide: V(98) Y L S E C K T G I G N G Y R G T M S(116)
Ordering Information
Pierce Angiostatin Antibody
Product #
PA1-600
Size
100 µg
Price
$360.00
Purchase
Add Angiostatin Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 2 µg/ml
Immunofluorescence (IF) 1:10-1:100
Immunocytochemistry (ICC) 1:10-1:100
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-600 detects recombinant human and mouse angiostatin.

PA1-600 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects a 50 kDa protein representing recombinant human angiostatin.

PA1-600 immunizing peptide corresponds to amino acid residues 98-116 from mouse angiostatin. This sequence is 95% and 84% conserved in rat and human angiostatin, respectively.
General Information
Angiostatin is a proteolytic fragment that comprises the first four kringle domains of the ~85 kDa plasminogen. Angiostatin reduces endothelial cell proliferation and acts as an potent inhibitor of angiogenesis and tumor growth.
Product Images
  • Angiostatin Antibody (PA1-600) in IF
    PA1-600_IF_NIH-3T3.jpg

    Immunofluorescence with anti-Angiostatin Polyclonal Antibody (PA1-600) (PA1-600_IF_NIH-3T3.jpg)

    Immunofluorescence with anti-Angiostatin Polyclonal Antibody (PA1-600)

    Immunofluorescent analysis of Angiostatin (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Angiostatin polyclonal antibody (Product # PA1-600) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • Angiostatin Antibody (PA1-600) in IF
    PA1-600_IF_K562.jpg

    Immunofluorescence with anti-Angiostatin Polyclonal Antibody (PA1-600) (PA1-600_IF_K562.jpg)

    Immunofluorescence with anti-Angiostatin Polyclonal Antibody (PA1-600)

    Immunofluorescent analysis of Angiostatin (green) showing staining in the cytoplasm of K562 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Angiostatin polyclonal antibody (Product # PA1-600) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific