Akt1 Antibody (G.145.7)

Akt1 Monoclonal Antibody for Western blot, IHC (P), IP, ChIP

>> See 31 other antibodies for AKT1 (RAC-PK-alpha; RAC-alpha serine/threonine-protein kinase; protein kinase B; proto-oncogene c-Akt; rac protein kinase alpha)
Synonyms:
RAC-PK-alpha; RAC-alpha serine/threonine-protein kinase; protein kinase B; proto-oncogene c-Akt; rac protein kinase alpha
Entrez Gene ID:
Details
Host / Isotype: Rabbit / IgG
Class: Monoclonal
Type: Antibody
Clone: G.145.7
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Non-human primate (Nhp)
Immunogen: Synthetic peptide surrounding Leu110 of human Akt1
Ordering Information
Pierce Akt1 Antibody (G.145.7)
Product #
MA5-14898
Size
100 ul
Price
$250.00
Purchase
Add Akt1 Antibody (G.145.7) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 1:400
Immunoprecipitation (IP) 1:50
ChIP assay (ChIP) 1-3 ul
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Purification: Affinity chromatography
Storage Buffer: 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol
Preservative: <0.02% sodium azide
Storage Conditions: -20°C
Product Specific Information
It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with Akt2 or Akt3.
General Information
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Product Images
  • Akt1 Antibody (MA5-14898) in WB
    MA5-14898_WesternBlot_2.jpg

    Western Blot with anti-Akt1 Monoclonal Antibody [G.145.7] (MA5-14898) (MA5-14898_WesternBlot_2.jpg)

    Western Blot with anti-Akt1 Monoclonal Antibody [G.145.7] (MA5-14898)

    Western blot analysis of Akt1 in extracts from Akt3 (lane 1) knock-out mouse embryonic fibroblasts (MEFs) and matched wild-type MEFs (lane 2) using Akt1 monoclonal antibody (Product # MA5-14898) (upper) or an Akt2 antibody (lower).
  • Akt1 Antibody (MA5-14898) in WB
    MA5-14898_WesternBlot_3.jpg

    Western Blot with anti-Akt1 Monoclonal Antibody [G.145.7] (MA5-14898) (MA5-14898_WesternBlot_3.jpg)

    Western Blot with anti-Akt1 Monoclonal Antibody [G.145.7] (MA5-14898)

    Western blot analysis of Akt1 in recombinant Akt1, Akt2 and Akt3 proteins using Akt1 monoclonal antibody (Product # MA5-14898) (upper) or an Akt (pan) monoclonal antibody (lower).
  • Akt1 Antibody (MA5-14898) in IHC
    MA5-14898_Immunohistochemistry_2.jpg

    Immunohistochemistry with anti-Akt1 Monoclonal Antibody [G.145.7] (MA5-14898) (MA5-14898_Immunohistochemistry_2.jpg)

    Immunohistochemistry with anti-Akt1 Monoclonal Antibody [G.145.7] (MA5-14898)

    Immunohistochemical analysis of Akt1 in paraffin-embedded LNCaP cells, untreated (left) or PI3K-Inhibitor-treated (right), using an Akt1 monoclonal antibody (Product # MA5-14898) using Phospho-Akt (Ser473) IHC Controls.
  • Akt1 Antibody (MA5-14898) in ChIP
    Akt1_Antibody_MA5-14898_ChIP_2_20130917094739.jpg

    ChIP assay with anti-Akt1 Monoclonal Antibody [G.145.7] (MA5-14898) (Akt1_Antibody_MA5-14898_ChIP_2_20130917094739.jpg)

    ChIP assay with anti-Akt1 Monoclonal Antibody [G.145.7] (MA5-14898)

    Chromatin immunoprecipitation analysis of Akt1 was performed using cross-linked chromatin from 1 x 106 HCT116 colon carcinoma cells treated with serum for 0, 15, 30, and 60 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0ul/100ul well volume of an Atk1 monoclonal antibody (Product # MA5-14898). Chromatin aliquots from ~1 x 105 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify -15kb upstream of the Egr1 gene or exon-1 of Egr1. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the Egr-1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions); the zigzag line represents an intron; and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.
  • Akt1 Antibody (MA5-14898) in ChIP
    Akt1_Antibody_MA5-14898_ChIP_1_20130917094835.jpg

    ChIP assay with anti-Akt1 Monoclonal Antibody [G.145.7] (MA5-14898) (Akt1_Antibody_MA5-14898_ChIP_1_20130917094835.jpg)

    ChIP assay with anti-Akt1 Monoclonal Antibody [G.145.7] (MA5-14898)

    Chromatin immunoprecipitation analysis of Akt1 and Akt2 was performed using cross-linked chromatin from 1 x 106 HCT116 colon carcinoma cells treated with serum for 0, 15, 30, and 60 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0ul/100ul well volume of an Atk1 monoclonal antibody (Product # MA5-14898) and an Akt2 monoclonal antibody (Product # MA1-034). Chromatin aliquots from ~1 x 105 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify -15kb upstream of the Egr1 gene or exon-1 of Egr1. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the Egr-1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions); the zigzag line represents an intron; and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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