Aggrecan G3 Antibody

Aggrecan G3 Polyclonal Antibody for Western blot

>> See 6 other antibodies for ACAN (AGC1, AGCAN, CSPG1, CSPGCP, MSK16, SEDK)
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Bovine (Bv), Canine (Ca)
Published Species Reactivity: Fish (Fs), Human (Hu)
Immunogen: Synthetic peptide corresponding to residues C D(2277) G H P M Q F E N W R P N Q P D N(2293) of human aggrecan.
Ordering Information
Pierce Aggrecan G3 Antibody
Product #
100 µg
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Tested Applications Dilution *
Western Blot (WB) 2 ug/ml
Published Applications Dilution
Western Blot (WB) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Affinity chromatography
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-1745 detects human, rat, mouse, bovine, and canine aggrecan G3.

PA1-1745 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~350 kDa protein representing the aggrecan G3 domain on the full length core.

The PA1-1745 immunogen is a synthetic peptide corresponding to residues C D(2277) G H P M Q F E N W R P N Q P D N(2293) of human aggrecan.. This antibody is also referred to as anti-G3.

General Information
Aggrecan is a member of large, aggregating proteoglycans (also including, versican, brevican and neurocan) found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMP’s) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis.

The G3 domain is a complicated region produced by alternative splicing of exons in post-transcriptional processes. The human G3 domain consists of three modules and articular cartilage contains a mixture of the alternatively spliced G3 modules. G3 has been shown to facilitate glycosaminoglycan (GAG) attachment and overall aggrecan processing. The G3 domain is central to aggrecan turnover and function in cartilage.
Product Images
  • Aggrecan G3 Antibody (PA1-1745) in WB

    Western Blot with anti-Aggrecan G3 Polyclonal Antibody (PA1-1745) (PA1-1745_1_ig.jpg)

    Western Blot with anti-Aggrecan G3 Polyclonal Antibody (PA1-1745)

    Figure 1 illustrates Western blot detection of mouse chondrocyte cells using PA1-1745. The specificity of PA1-1745 is demonstrated in lane 1. Lane 1: mouse chondrocyte Lane 2: a lane of human cartilage proteoglycan blotted with anti-aggrecan G1 domain (general)
Western Blot
  Species / Dilution Summary
  Fs / Not Cited
PA1-1745 was used in western blot to investigate the structural composition of proteoglycan in salmon nasal cartilage

Arch Biochem Biophys. 2011 Feb 1;506(1):58-65.
"Identification of proteoglycan from salmon nasal cartilage."
Author(s): Kakizaki I, Tatara Y, Majima M, Kato Y, Endo M
Number of Citations: 0
(See PubMed article )
  Hu / 1:500
PA1-1745 was used in western blot to evaluate the effectiveness of various purification methods of aggrecan purification from human cartilage and synovial fluid

Matrix Biol. 2010 Jan;29(1):74-83.
"A comparison of different purification methods of aggrecan fragments from human articular cartilage and synovial fluid."
Author(s): Struglics A, Larsson S
Number of Citations: 0
(See PubMed article )
  Hu / 1:500
PA1-1745 was used in western blot to study the role oh calpain in the truncation of human aggrecan.

Biochem J. 2010 Sep 15;430(3):531-8.
"Calpain is involved in C-terminal truncation of human aggrecan."
Author(s): Struglics A, Hansson M
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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