Actin Antibody (mAbGEa)

Actin Monoclonal Antibody for Western blot, IF, IHC, ELISA

Synonyms:
ACTA, ACTA1, alpha Actin 1, alpha skeletal muscle Actin, ASMA, NEM1, NEM2
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: mAbGEa
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Bovine (Bv), Drosophila (Dm), Ovine (Ov), Plant (Pl), Xenopus laevis (Xl), Yeast (Ys), Zebrafish (Zf)
Published Species Reactivity: Bacteria (Ba), Canine (Ca), Hamster (Hm), Human (Hu), Mouse (Ms), Not Applicable (N/A), Plant (Pl), Porcine (Po), Rat (Rt), Xenopus laevis (Xl)
Immunogen: Purified Arabidopsis actin protein.
Ordering Information
Pierce Actin Antibody (mAbGEa)
Product #
MA1-744
Size
100 µl
Price
$360.00
Purchase
Add Actin Antibody (mAbGEa) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1,000
Immunofluorescence (IF) 1:10-1:100
Immunohistochemistry (IHC) 1:100
ELISA (ELISA) Assay dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
ELISA (ELISA) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: ascites
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA1-744 detects actin protein in human, mouse, rat, bovine, sheep, drosophila, zebra fish (Danio rerio), yeast, arabidopsis and xenopus samples. This antibody has shown cross-reactivity with actin 1, 2, 3, 4, 7, 8, 11 and 12.

MA1-744 has successfully been used in Western blot and ELISA procedures. By Western blot, this antibody detects a 45 kDa protein representing actin.

The MA1-744 immunogen is purified actin protein from Arabidopsis.

General Information
Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton.  Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis.  In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Product Images
  • Actin Antibody (MA1-744) in WB
    ACTC1_Antibody_MA1-744_WesternBlot(WB).jpg

    Western Blot with anti-Actin Monoclonal Antibody [mAbGEa] (MA1-744) (ACTC1_Antibody_MA1-744_WesternBlot(WB).jpg)

    Western Blot with anti-Actin Monoclonal Antibody [mAbGEa] (MA1-744)

    Western blot detection of actin in MDCK cell extract using MA1-744.
  • Actin Antibody (MA1-744) in IF
    Actin_MA1-744_Immunofluorescence_A375-Cells.jpg

    Immunofluorescence with anti-Actin Monoclonal Antibody [mAbGEa] (MA1-744) (Actin_MA1-744_Immunofluorescence_A375-Cells.jpg)

    Immunofluorescence with anti-Actin Monoclonal Antibody [mAbGEa] (MA1-744)

    Immunofluorescent analysis of Actin using Anti-Actin Monoclonal Antibody (mAbGEa) (Product# MA1-744) shows staining in A375 Cells. Actin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Actin (Product# MA1-744) at a dilution of 1:20 over night at 4 ◦C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
  • Actin Antibody (MA1-744) in IF
    Actin_MA1-744_Immunofluorescence_Hela-Cells.jpg

    Immunofluorescence with anti-Actin Monoclonal Antibody [mAbGEa] (MA1-744) (Actin_MA1-744_Immunofluorescence_Hela-Cells.jpg)

    Immunofluorescence with anti-Actin Monoclonal Antibody [mAbGEa] (MA1-744)

    Immunofluorescent analysis of Actin using Anti-Actin Monoclonal Antibody (mAbGEa) (Product# MA1-744) shows staining in Hela Cells. Actin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Actin (Product# MA1-744) at a dilution of 1:20 over night at 4 ◦C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
  • Actin Antibody (MA1-744) in IHC
    MA1-744_Immunohistochemistry_Colon carcinoma.jpg

    Immunohistochemistry with anti-Actin Monoclonal Antibody [mAbGEa] (MA1-744) (MA1-744_Immunohistochemistry_Colon carcinoma.jpg)

    Immunohistochemistry with anti-Actin Monoclonal Antibody [mAbGEa] (MA1-744)

    Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Anti-Actin (MA1-744) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Actin Antibody (MA1-744) in IHC
    MA1-744_Immunohistochemistry_Skeletal muscle.jpg

    Immunohistochemistry with anti-Actin Monoclonal Antibody [mAbGEa] (MA1-744) (MA1-744_Immunohistochemistry_Skeletal muscle.jpg)

    Immunohistochemistry with anti-Actin Monoclonal Antibody [mAbGEa] (MA1-744)

    Immunohistochemistry was performed on normal deparaffinized Human skeletal muscle tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Anti-Actin (MA1-744) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Actin Antibody (MA1-744) in IHC
    MA1-744_Immunohistochemistry_Tonsil tissue.jpg

    Immunohistochemistry with anti-Actin Monoclonal Antibody [mAbGEa] (MA1-744) (MA1-744_Immunohistochemistry_Tonsil tissue.jpg)

    Immunohistochemistry with anti-Actin Monoclonal Antibody [mAbGEa] (MA1-744)

    Immunohistochemistry was performed on normal deparaffinized Human tonsil tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Anti-Actin (MA1-744) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Ba / 0
MA1-744 was used in western blot to characterize the eukaryote-type actin and profilin expressed in cyanobacteria

PLoS One. 2012;7(1):e29926.
"Unique properties of eukaryote-type actin and profilin horizontally transferred to cyanobacteria."
Author(s): Guljamow A, Delissen F, Baumann O, Thünemann AF, Dittmann E
Number of Citations: 1
(See PubMed article )
  Ca / 1:2000
MA1-744 was used in western blot to study hepatitis and cirrhosis in dogs as potential models of human liver fibrosis

Liver Int. 2006 Aug;26(6):716-25.
"Transforming growth factor beta-1 signalling in canine hepatic diseases: new models for human fibrotic liver pathologies."
Author(s): Spee B, Arends B, van den Ingh TS, Brinkhof B, Nederbragt H, Ijzer J, Roskams T, Penning LC, Rothuizen J
Number of Citations: 5
(See PubMed article )
  Hm / 0
MA1-744 was used in western blot to study the role of phosphorylation of Tyr-267 of keratin-8 in modulating solubility and intermediate filament formation

J Biol Chem. 2013 Oct 25;288(43):31329-37.
"A conserved rod domain phosphotyrosine that is targeted by the phosphatase PTP1B promotes keratin 8 protein insolubility and filament organization."
Author(s): Snider NT, Park H, Omary MB
Number of Citations: 0
(See PubMed article )
  Hu / 1 ug/ml
MA1-744 was used in western blot to study the role of fibronectin-inducible COX-2 and PGE2 in rhabdomyosarcoma invasiveness and MMP-2 expression

Biochem Biophys Res Commun. 2004 May 28;318(2):594-600.
"Fibronectin-induced COX-2 mediates MMP-2 expression and invasiveness of rhabdomyosarcoma."
Author(s): Ito H, Duxbury M, Benoit E, Farivar RS, Gardner-Thorpe J, Zinner MJ, Ashley SW, Whang EE
Number of Citations: 2
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study the involvement of Apo2/TRAIL in Dengue virus-induced apoptosis of hepatic HepG2 cells

J Gen Virol. 2005 Apr;86(Pt 4):1055-65.
"Dengue virus-induced apoptosis in hepatic cells is partly mediated by Apo2 ligand/tumour necrosis factor-related apoptosis-inducing ligand."
Author(s): Matsuda T, Almasan A, Tomita M, Tamaki K, Saito M, Tadano M, Yagita H, Ohta T, Mori N
Number of Citations: 10
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study the ability of a HSP90 inhibitor to promote osteoclast formation and bone metastasis of human breast cancer cells

Cancer Res. 2005 Jun 1;65(11):4929-38.
"The heat shock protein 90 inhibitor, 17-allylamino-17-demethoxygeldanamycin, enhances osteoclast formation and potentiates bone metastasis of a human breast cancer cell line."
Author(s): Price JT, Quinn JM, Sims NA, Vieusseux J, Waldeck K, Docherty SE, Myers D, Nakamura A, Waltham MC, Gillespie MT, Thompson EW
Number of Citations: 26
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study the mechanism underlying STAT1-mediated expression of phospholipd scramblase-1 in response to IFN-alpha

J Biol Chem. 2005 Dec 30;280(52):42707-14.
"Interferon-alpha-induced expression of phospholipid scramblase 1 through STAT1 requires the sequential activation of protein kinase Cdelta and JNK."
Author(s): Zhao KW, Li D, Zhao Q, Huang Y, Silverman RH, Sims PJ, Chen GQ
Number of Citations: 8
(See PubMed article )
  Hu / 1:2000
MA1-744 was used in western blot to study hepatitis and cirrhosis in dogs as potential models of human liver fibrosis

Liver Int. 2006 Aug;26(6):716-25.
"Transforming growth factor beta-1 signalling in canine hepatic diseases: new models for human fibrotic liver pathologies."
Author(s): Spee B, Arends B, van den Ingh TS, Brinkhof B, Nederbragt H, Ijzer J, Roskams T, Penning LC, Rothuizen J
Number of Citations: 5
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study the role of beta-catenin signaling in the transformation of human T-cells by HTLV-1

J Virol. 2006 Nov;80(21):10497-505.
"Human T-cell leukemia virus type 1 tax dysregulates beta-catenin signaling."
Author(s): Tomita M, Kikuchi A, Akiyama T, Tanaka Y, Mori N
Number of Citations: 11
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study the involvement of the mitochondrial pathway in the induction of hepatoma apoptosis by the topoisomerase I inhibitor hydroxycamptothecin

Mitochondrion. 2006 Aug;6(4):211-7.
"Hydroxycamptothecin-induced apoptosis in hepatoma SMMC-7721 cells and the role of mitochondrial pathway."
Author(s): Fu YR, Yi ZJ, Yan YR, Qiu ZY
Number of Citations: 1
(See PubMed article )
  Hu / 1:500
MA1-744 was used in western blot to study the modulation of the nucleotide excision-repair factor DDB2 by the replication checkpoint protein Claspin

DNA Repair (Amst). 2007 May 1;6(5):578-87.
"Role of Claspin in regulation of nucleotide excision repair factor DDB2."
Author(s): Praetorius-Ibba M, Wang QE, Wani G, El-Mahdy MA, Zhu Q, Qin S, Wani AA
Number of Citations: 5
(See PubMed article )
  Hu / 1:10000
MA1-744 was used in western blot to characterize Apin

J Cell Biochem. 2008 Feb 15;103(3):941-56.
"Characterization of Apin, a secreted protein highly expressed in tooth-associated epithelia."
Author(s): Moffatt P, Smith CE, St-Arnaud R, Nanci A
Number of Citations: 18
(See PubMed article )
  Hu / 1:2000
MA1-744 was used in western blot to study the similarities in HGF-mediated regeneration pathway activation in cirrhosis in humans and dogs

Comp Hepatol. 2007 Jul 31;6():8.
"Major HGF-mediated regenerative pathways are similarly affected in human and canine cirrhosis."
Author(s): Spee B, Arends B, van den Ingh TS, Roskams T, Rothuizen J, Penning LC
Number of Citations: 2
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to examine the effects of autistic blood serum on development of neuronal progenitor cells in vitro

Brain Res. 2007 Sep 7;1168():11-20.
"Altered development of neuronal progenitor cells after stimulation with autistic blood sera."
Author(s): Mazur-Kolecka B, Cohen IL, Jenkins EC, Kaczmarski W, Flory M, Frackowiak J
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study the role of the mitochondrial pathway in the induction of apoptosis in hepatoma cells by intracellular granulysin

Cell Immunol. 2009;255(1-2):76-81.
"Intracellularly expressed granulysin induced apoptosis in hepatoma cells and role of mitochondrial apoptotic pathway."
Author(s): Yi Z, Fu Y, Jin G, Li M, Zhang X, Song W
Number of Citations: 0
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study the increased proliferation and invasiveness of human mammary epithelial cells expressing degradation-resistant prolactin receptor

Cancer Res. 2009 Apr 1;69(7):3165-72.
"Impaired turnover of prolactin receptor contributes to transformation of human breast cells."
Author(s): Plotnikov A, Varghese B, Tran TH, Liu C, Rui H, Fuchs SY
Number of Citations: 9
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study the ability of cells to cope with SNARE depletion

Traffic. 2009 Oct;10(10):1543-59.
"Endosomal fusion upon SNARE knockdown is maintained by residual SNARE activity and enhanced docking."
Author(s): Bethani I, Werner A, Kadian C, Geumann U, Jahn R, Rizzoli SO
Number of Citations: 6
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study the role of kinesin overexpression in the resistance of breast cancer cells to docetaxol

Cancer Res. 2009 Oct 15;69(20):8035-42.
"Overexpression of kinesins mediates docetaxel resistance in breast cancer cells."
Author(s): De S, Cipriano R, Jackson MW, Stark GR
Number of Citations: 8
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study the role of caveolin-1 overexpression in adult T-cell leukemia

Blood. 2010 Mar 18;115(11):2220-30.
"Overexpression of caveolin-1 in adult T-cell leukemia."
Author(s): Sawada S, Ishikawa C, Tanji H, Nakachi S, Senba M, Okudaira T, Uchihara JN, Taira N, Ohshiro K, Yamada Y, Tanaka Y, Uezato H, Ohshima K, Sasai K, Burgering BM, Duc Dodon M, Fujii M, Sunakawa H, Mori N
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study the role of IL-1RI and Rho kinase in the mechanism by which Helicobacter pylori infection disrupts gastric epithelial tight junctions

Cell Microbiol. 2010 May 1;12(5):692-703.
"Interleukin-1 receptor phosphorylation activates Rho kinase to disrupt human gastric tight junctional claudin-4 during Helicobacter pylori infection."
Author(s): Lapointe TK, O'Connor PM, Jones NL, Menard D, Buret AG
Number of Citations: 7
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study the effects of human CMV infection on the differentiation of human neural progenitor cells

J Virol. 2010 Apr;84(7):3528-41.
"Human cytomegalovirus infection causes premature and abnormal differentiation of human neural progenitor cells."
Author(s): Luo MH, Hannemann H, Kulkarni AS, Schwartz PH, O'Dowd JM, Fortunato EA
Number of Citations: 15
(See PubMed article )
  Hu / 1:1000
MA1-744 was used in western blot to identify novel small molecule inhbitors of apurinic/apyrimidinic endonuclease-1 and study their therapeutic potential in glioblastoma

J Pharmacol Exp Ther. 2010 Sep 1;334(3):988-98.
"Novel small-molecule inhibitor of apurinic/apyrimidinic endonuclease 1 blocks proliferation and reduces viability of glioblastoma cells."
Author(s): Bapat A, Glass LS, Luo M, Fishel ML, Long EC, Georgiadis MM, Kelley MR
Number of Citations: 25
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study the effect of wild-type MLL on the response to DNA damage and the sensitivity to glucocorticoids in pediatric acute lymphoblastic leukemia

Mol Cancer. 2010 Oct 28;9():284.
"Influence of wild-type MLL on glucocorticoid sensitivity and response to DNA-damage in pediatric acute lymphoblastic leukemia."
Author(s): Beesley AH, Rampellini JL, Palmer ML, Heng JY, Samuels AL, Firth MJ, Ford J, Kees UR
Number of Citations: 0
(See PubMed article )
  Hu / 1:500
MA1-744 was used in western blot to study the transcriptional regulation of MIP3-alpha by KLF6 and PPAR-gamma and the significance for diabetic retinopathy

Int J Biochem Cell Biol. 2011 Mar;43(3):383-92.
"The roles of Kruppel-like factor 6 and peroxisome proliferator-activated receptor-? in the regulation of macrophage inflammatory protein-3? at early onset of diabetes."
Author(s): Qi W, Holian J, Tan CY, Kelly DJ, Chen XM, Pollock CA
Number of Citations: 0
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study Mdm2-mediated regulation of p53

Cell Death Differ. 2011 Jun;18(6):1005-15.
"SUMO-specific protease 2 in Mdm2-mediated regulation of p53."
Author(s): Jiang M, Chiu SY, Hsu W
Number of Citations: 8
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to develop and evaluate a high content imaging-based assay for analyzing estrogen receptor ligands

Gene. 2011 May 15;477(1-2):42-52.
"High content imaging-based assay to classify estrogen receptor-? ligands based on defined mechanistic outcomes."
Author(s): Ashcroft FJ, Newberg JY, Jones ED, Mikic I, Mancini MA
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study the molecular mechanism by which HTLV-1 transactivates MMP-7 gene expression

Biochim Biophys Acta. 2011 May;1813(5):731-41.
"Human T-cell leukemia virus type 1 tax transactivates the matrix metalloproteinase 7 gene via JunD/AP-1 signaling."
Author(s): Nakachi S, Nakazato T, Ishikawa C, Kimura R, Mann DA, Senba M, Masuzaki H, Mori N
Number of Citations: 0
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to characterize alterations in calcium-sensing receptor in colorectal carcinogenesis

Mod Pathol. 2011 Jun;24(6):876-84.
"Epigenetic inactivation of calcium-sensing receptor in colorectal carcinogenesis."
Author(s): Hizaki K, Yamamoto H, Taniguchi H, Adachi Y, Nakazawa M, Tanuma T, Kato N, Sukawa Y, Sanchez JV, Suzuki H, Sasaki S, Imai K, Shinomura Y
Number of Citations: 4
(See PubMed article )
  Hu / 1:1000
MA1-744 was used in western blot to study the mechanism of the anti-tumor activity of the EGFR inhibitor cetuximab

BMC Res Notes. 2011 May 10;4():140.
"Analysis of the anti-tumor effect of cetuximab using protein kinetics and mouse xenograft models."
Author(s): Matsuo T, Nishizuka SS, Ishida K, Iwaya T, Ikeda M, Wakabayashi G
Number of Citations: 3
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study the conservation of VEGF-R family signalling in lymphocytic endothelial cells from mice

Exp Cell Res. 2011 Oct 15;317(17):2397-407.
"Conserved signaling through vascular endothelial growth (VEGF) receptor family members in murine lymphatic endothelial cells."
Author(s): Coso S, Zeng Y, Sooraj D, Williams ED
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study the role of toll-like receptors in diabetic nephropathy

J Am Soc Nephrol. 2012 Jan;23(1):86-102.
"Toll-like receptor 4 promotes tubular inflammation in diabetic nephropathy."
Author(s): Lin M, Yiu WH, Wu HJ, Chan LY, Leung JC, Au WS, Chan KW, Lai KN, Tang SC
Number of Citations: 8
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study the conserved functional competence of cytoplasmic actins

Plant Cell. 2012 May;24(5):2041-57.
"Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists."
Author(s): Kandasamy MK, McKinney EC, Roy E, Meagher RB
Number of Citations: 1
(See PubMed article )
  Hu / 1:1000
MA1-744 was used in western blot to study the role of HSF1 in driving a transcriptional program distinct from heat shock in highly malignant human cancers

Cell. 2012 Aug 3;150(3):549-62.
"HSF1 drives a transcriptional program distinct from heat shock to support highly malignant human cancers."
Author(s): Mendillo ML, Santagata S, Koeva M, Bell GW, Hu R, Tamimi RM, Fraenkel E, Ince TA, Whitesell L, Lindquist S
Number of Citations: 14
(See PubMed article )
  Hu / 1:10000
MA1-744 was used in western blot to study the effect of imatinib on separase proteolytic activity in BCR-ABL-positive and -negative cells

PLoS One. 2012;7(8):e42863.
"The proteolytic activity of separase in BCR-ABL-positive cells is increased by imatinib."
Author(s): Haaß W, Stehle M, Nittka S, Giehl M, Schrotz-King P, Fabarius A, Hofmann WK, Seifarth W
Number of Citations: 0
(See PubMed article )
  Hu / 1:1000
MA1-744 was used in western blot to compare the molecular mechanisms of the cytolethal distending toxins from Escherichia coli and Haemophilus ducreyi

J Biol Chem. 2013 Mar 15;288(11):7492-505.
"Cellular interactions of the cytolethal distending toxins from Escherichia coli and Haemophilus ducreyi."
Author(s): Gargi A, Tamilselvam B, Powers B, Prouty MG, Lincecum T, Eshraghi A, Maldonado-Arocho FJ, Wilson BA, Bradley KA, Blanke SR
Number of Citations: 0
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study the prognostic significance of immunohistochemical expression of cytoplasmic CSE1L in colorectal cancer

J Transl Med. 2013 Jan 31;11():29.
"Correlations between cytoplasmic CSE1L in neoplastic colorectal glands and depth of tumor penetration and cancer stage."
Author(s): Tai CJ, Su TC, Jiang MC, Chen HC, Shen SC, Lee WR, Liao CF, Chen YC, Lin SH, Li LT, Shen KH, Yeh CM, Yeh KT, Lee CH, Shih HY, Chang CC
Number of Citations: 0
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to study the role of phosphorylation of Tyr-267 of keratin-8 in modulating solubility and intermediate filament formation

J Biol Chem. 2013 Oct 25;288(43):31329-37.
"A conserved rod domain phosphotyrosine that is targeted by the phosphatase PTP1B promotes keratin 8 protein insolubility and filament organization."
Author(s): Snider NT, Park H, Omary MB
Number of Citations: 0
(See PubMed article )
  Hu / 0
MA1-744 was used in western blot to determine how HER2/HER3 regulates extracellular acidification and cell migration

Cell Signal. 2014 Jan;26(1):70-82.
"HER2/HER3 regulates extracellular acidification and cell migration through MTK1 (MEKK4)."
Author(s): Sollome JJ, Thavathiru E, Camenisch TD, Vaillancourt RR
Number of Citations: 0
(See PubMed article )
  Ms / 0
MA1-744 was used in western blot to study the effect of Txk/Rlk tyrosine kinase expression on the murine Th1/Th2 cytokine balance and serum IgE levels

Clin Exp Allergy. 2004 Jun;34(6):965-70.
"Th1-dominant shift of T cell cytokine production, and subsequent reduction of serum immunoglobulin E response by administration in vivo of plasmid expressing Txk/Rlk, a member of Tec family tyrosine kinases, in a mouse model."
Author(s): Takeno M, Yoshikawa H, Kurokawa M, Takeba Y, Kashiwakura JI, Sakaguchi M, Yasueda H, Suzuki N
Number of Citations: 2
(See PubMed article )
  Ms / 0
MA1-744 was used in western blot to study the role of HTLV-1 tax in the transcriptional activation of survivin and the involvement of the NFkappaB pathway

Int J Cancer. 2005 Jul 20;115(6):967-74.
"Transcriptional activation of survivin through the NF-kappaB pathway by human T-cell leukemia virus type I tax."
Author(s): Kawakami H, Tomita M, Matsuda T, Ohta T, Tanaka Y, Fujii M, Hatano M, Tokuhisa T, Mori N
Number of Citations: 24
(See PubMed article )
  Ms / 1:2000
MA1-744 was used in western blot to examine the effects of sulforaphane on photoreceptor degeneration in the tubby mouse

J Neurochem. 2007 May;101(4):1041-52.
"Delay of photoreceptor degeneration in tubby mouse by sulforaphane."
Author(s): Kong L, Tanito M, Huang Z, Li F, Zhou X, Zaharia A, Yodoi J, McGinnis JF, Cao W
Number of Citations: 14
(See PubMed article )
  Ms / 0
MA1-744 was used in western blot to investigate possible links between cholesterol secoaldehyde and amyloid aggregation

J Alzheimers Dis. 2007 Jun;11(3):261-74.
"Cholesterol secoaldehyde, an ozonation product of cholesterol, induces amyloid aggregation and apoptosis in murine GT1-7 hypothalamic neurons."
Author(s): Sathishkumar K, Xi X, Martin R, Uppu RM
Number of Citations: 3
(See PubMed article )
  Ms / 1:1000
MA1-744 was used in western blot to investigate the role of autophagy in the intracellular survival of Burkholderia pseudomallei

Autophagy. 2008 Aug;4(6):744-53.
"Stimulation of autophagy suppresses the intracellular survival of Burkholderia pseudomallei in mammalian cell lines."
Author(s): Cullinane M, Gong L, Li X, Lazar-Adler N, Tra T, Wolvetang E, Prescott M, Boyce JD, Devenish RJ, Adler B
Number of Citations: 20
(See PubMed article )
  Ms / 0
MA1-744 was used in western blot to study the effects of Citrobacter rodentium on mouse intestinal epithelial cell tight junction protein expression and apoptosis

Microb Pathog. 2008 Aug;45(2):98-104.
"Tight junctional disruption and apoptosis in an in vitro model of Citrobacter rodentium infection."
Author(s): Flynn AN, Buret AG
Number of Citations: 7
(See PubMed article )
  Ms / 1:500
MA1-744 was used in western blot to investigate blood-retina barrier permeability in experimental endophthalmitis

Invest Ophthalmol Vis Sci. 2009 Aug;50(8):3783-93.
"Bacillus cereus-induced permeability of the blood-ocular barrier during experimental endophthalmitis."
Author(s): Moyer AL, Ramadan RT, Novosad BD, Astley R, Callegan MC
Number of Citations: 10
(See PubMed article )
  Ms / 1:3333
MA1-744 was used in western blot to study the mechanism by which SLIMMER delays apoptosis of skeletal muscle myoblasts

J Biol Chem. 2009 Sep 25;284(39):26964-77.
"SLIMMER (FHL1B/KyoT3) interacts with the proapoptotic protein Siva-1 (CD27BP) and delays skeletal myoblast apoptosis."
Author(s): Cottle DL, McGrath MJ, Wilding BR, Cowling BS, Kane JM, D'Arcy CE, Holdsworth M, Hatzinisiriou I, Prescott M, Brown S, Mitchell CA
Number of Citations: 2
(See PubMed article )
  Ms / 0
MA1-744 was used in western blot to study the role of cellular microRNAs in regulating the generation of inducible pluripotent stem cells

EMBO J. 2011 Mar 2;30(5):823-34.
"Small RNA-mediated regulation of iPS cell generation."
Author(s): Li Z, Yang CS, Nakashima K, Rana TM
Number of Citations: 54
(See PubMed article )
  Ms / 1:1000
MA1-744 was used in western blot to investigate the effect of chronic brimonidine treatment on retinal and choroidal neovascularization

Invest Ophthalmol Vis Sci. 2011 Jul;52(8):5424-31.
"Effect of brimonidine on retinal and choroidal neovascularization in a mouse model of retinopathy of prematurity and laser-treated rats."
Author(s): Kusari J, Padillo E, Zhou SX, Bai Y, Wang J, Song Z, Zhu M, Le YZ, Gil DW
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA1-744 was used in western blot to study the effects of dietary restriction and PGC-1alpha deletion on age-related decreases in oocyte quality in mice

Proc Natl Acad Sci U S A. 2011 Jul 26;108(30):12319-24.
"Prevention of maternal aging-associated oocyte aneuploidy and meiotic spindle defects in mice by dietary and genetic strategies."
Author(s): Selesniemi K, Lee HJ, Muhlhauser A, Tilly JL
Number of Citations: 12
(See PubMed article )
  Ms / 0
MA1-744 was used in western blot to study the molecular mechanism underlying the supression of HES-1 by GATA-1 and the significance for erythropoiesis

Mol Cell Biol. 2012 Sep;32(18):3624-38.
"GATA-1 utilizes Ikaros and polycomb repressive complex 2 to suppress Hes1 and to promote erythropoiesis."
Author(s): Ross J, Mavoungou L, Bresnick EH, Milot E
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA1-744 was used in western blot to study the role of phosphorylation of Tyr-267 of keratin-8 in modulating solubility and intermediate filament formation

J Biol Chem. 2013 Oct 25;288(43):31329-37.
"A conserved rod domain phosphotyrosine that is targeted by the phosphatase PTP1B promotes keratin 8 protein insolubility and filament organization."
Author(s): Snider NT, Park H, Omary MB
Number of Citations: 0
(See PubMed article )
  N/A / 0
MA1-744 was used in western blot to characterize RIG-1-like receptors in Rainbow trout

J Virol. 2011 Aug;85(16):8403-12.
"Expression and functional characterization of the RIG-I-like receptors MDA5 and LGP2 in Rainbow trout (Oncorhynchus mykiss)."
Author(s): Chang M, Collet B, Nie P, Lester K, Campbell S, Secombes CJ, Zou J
Number of Citations: 7
(See PubMed article )
  Pl / Not Cited
MA1-744 was used in ELISA, immunohistochemistry and western blot to study the actin-gene family of Arabidopsis in angiosperms

Plant J. 1999 Jun;18(6):681-91.
"The late pollen-specific actins in angiosperms."
Author(s): Kandasamy MK, McKinney EC, Meagher RB
Number of Citations: 14
(See PubMed article )
  Pl / Not Cited
MA1-744 was used in western blot to characterize a new plant P loop NTPase from Arabidopsis.

J Biol Chem. 2008 Dec 19;283(51):35797-804.
"The essential cytosolic iron-sulfur protein Nbp35 acts without Cfd1 partner in the green lineage."
Author(s): Bych K, Netz DJ, Vigani G, Bill E, Lill R, Pierik AJ, Balk J
Number of Citations: 1
(See PubMed article )
  Pl / Not Cited
MA1-744 was used in western blot to study the functions of the mitochondrial ATP-binding cassette transporters.

Plant Physiol. 2009 Oct;151(2):590-602.
"An allelic mutant series of ATM3 reveals its key role in the biogenesis of cytosolic iron-sulfur proteins in Arabidopsis."
Author(s): Bernard DG, Cheng Y, Zhao Y, Balk J
Number of Citations: 1
(See PubMed article )
  Pl / 0
MA1-744 was used in immunocytochemistry and western blot to investigate the distribution of different actin proteins in the nucleus

Cytoskeleton (Hoboken). 2010 Nov;67(11):729-43.
"Differential sublocalization of actin variants within the nucleus."
Author(s): Kandasamy MK, McKinney EC, Meagher RB
Number of Citations: 1
(See PubMed article )
  Pl / 1:1000
MA1-744 was used in western blot to investigate the functions of chitinase-like1/pom-pom1 and CTL2 in cellulose biosynthesis

Plant Cell. 2012 Feb;24(2):589-607.
"Chitinase-like1/pom-pom1 and its homolog CTL2 are glucan-interacting proteins important for cellulose biosynthesis in Arabidopsis."
Author(s): Sánchez-Rodríguez C, Bauer S, Hématy K, Saxe F, Ibáñez AB, Vodermaier V, Konlechner C, Sampathkumar A, Rüggeberg M, Aichinger E, Neumetzler L, Burgert I, Somerville C, Hauser MT, Persson S
Number of Citations: 9
(See PubMed article )
  Po / 1:1000
MA1-744 was used in western blot to study the beneficial effects of inhibiting TNF-alpha and IL-1beta production in promoting recovery from renal reperfusion injury

J Vasc Surg. 2009 Mar;49(3):728-40.
"FR167653 improves renal recovery and decreases inflammation and fibrosis after renal ischemia reperfusion injury."
Author(s): Cau J, Favreau F, Zhang K, Febrer G, de la Motte GR, Ricco JB, Goujon JM, Hauet T
Number of Citations: 6
(See PubMed article )
  Po / 1:1000
MA1-744 was used in western blot to study the effect of inhibition of p38 MAPK and TNF-alpha production on damage caused by renal warm ischemia

Br J Surg. 2009 Jul;96(7):799-808.
"Influence of nephron mass and a phosphorylated 38 mitogen-activated protein kinase inhibitor on the development of early and long-term injury after renal warm ischaemia."
Author(s): Jayle C, Faure JP, Thuillier R, Goujon JM, Richer JP, Hauet T
Number of Citations: 3
(See PubMed article )
  Rt / 1:1000
MA1-744 was used in western blot to study the different MAP kinase pathways activated in renal epithelial, endothelial and fibroblast cells in response to fibrogenic stress

Nephrology (Carlton). 2003 Aug;8(4):196-204.
"Fibrogenic stresses activate different mitogen-activated protein kinase pathways in renal epithelial, endothelial or fibroblast cell populations."
Author(s): Pat BK, Cuttle L, Watters D, Yang T, Johnson DW, Gobe GC
Number of Citations: 1
(See PubMed article )
  Rt / 0
MA1-744 was used in western blot to study the effects of gastrin on the recovery of murine intestinal crypts from injury

Gastroenterology. 2006 Apr;130(4):1169-80.
"Gastrin increases murine intestinal crypt regeneration following injury."
Author(s): Ottewell PD, Duckworth CA, Varro A, Dimaline R, Wang TC, Watson AJ, Dockray GJ, Pritchard DM
Number of Citations: 2
(See PubMed article )
  Rt / 1:1000
MA1-744 was used in western blot to study the role of insulin receptor signaling in rod photoreceptor cells

J Biol Chem. 2008 Jul 11;283(28):19781-92.
"Loss of neuroprotective survival signal in mice lacking insulin receptor gene in rod photoreceptor cells."
Author(s): Rajala A, Tanito M, Le YZ, Kahn CR, Rajala RV
Number of Citations: 27
(See PubMed article )
  Rt / 1:1000
MA1-744 was used in western blot to examine IGFR-1 signaling in rod outer segment membranes

Invest Ophthalmol Vis Sci. 2008 Nov;49(11):4765-73.
"Insulin growth factor 1 receptor/PI3K/AKT survival pathway in outer segment membranes of rod photoreceptors."
Author(s): Dilly AK, Rajala RV
Number of Citations: 2
(See PubMed article )
  Rt / 0
MA1-744 was used in western blot to examine retinal insulin receptor signaling in hyperosmotic stress

Vitam Horm. 2009;80():583-612.
"Retinal insulin receptor signaling in hyperosmotic stress."
Author(s): Rajala RV, Ivanovic I, Dilly AK
Number of Citations: 3
(See PubMed article )
  Rt / 1:1000
MA1-744 was used in western blot to study insulin receptor signaling in developing photoreceptors

J Neurochem. 2009 Sep;110(5):1648-60.
"Insulin receptor signaling regulates actin cytoskeletal organization in developing photoreceptors."
Author(s): Rajala RV, Rajala A, Brush RS, Rotstein NP, Politi LE
Number of Citations: 1
(See PubMed article )
  Rt / 0
MA1-744 was used in western blot to study the interdependence of PDGF and estrogen signaling and their role in gonocyte development

Biol Reprod. 2010 May;82(5):825-36.
"Interdependence of platelet-derived growth factor and estrogen-signaling pathways in inducing neonatal rat testicular gonocytes proliferation."
Author(s): Thuillier R, Mazer M, Manku G, Boisvert A, Wang Y, Culty M
Number of Citations: 4
(See PubMed article )
  Rt / 1:1000
MA1-744 was used in western blot to study protein phosphorylation in the retina

J Proteomics Bioinform. 2008 Aug 13;1():242.
"Phospho-Site-Specific Antibody Microarray to Study the State of Protein Phosphorylation in the Retina."
Author(s): Rajala RV
Number of Citations: 0
(See PubMed article )
  Rt / 1:500
MA1-744 was used in western blot to study the short-term regulation of intestinal SGLT-1 by glucose and sweet taste molecules

Ann Surg. 2010 May;251(5):865-71.
"Rapid upregulation of sodium-glucose transporter SGLT1 in response to intestinal sweet taste stimulation."
Author(s): Stearns AT, Balakrishnan A, Rhoads DB, Tavakkolizadeh A
Number of Citations: 9
(See PubMed article )
  Rt / 1:500
MA1-744 was used in western blot to study whether a synthetic flavonol antioxidant can protect against diabetic cardiomyopathy in a rat model of diabetes

PLoS One. 2011;6(7):e22777.
"3',4'-Dihydroxyflavonol antioxidant attenuates diastolic dysfunction and cardiac remodeling in streptozotocin-induced diabetic m(Ren2)27 rats."
Author(s): Khong FL, Zhang Y, Edgley AJ, Qi W, Connelly KA, Woodman OL, Krum H, Kelly DJ
Number of Citations: 2
(See PubMed article )
  Rt / 1:500
MA1-744 was used in western blot to study the role of different afferent vagal fibres in mediating resistance to diet-induced obesity in a rat model

Dig Dis Sci. 2012 May;57(5):1281-90.
"Relative contributions of afferent vagal fibers to resistance to diet-induced obesity."
Author(s): Stearns AT, Balakrishnan A, Radmanesh A, Ashley SW, Rhoads DB, Tavakkolizadeh A
Number of Citations: 2
(See PubMed article )
  Rt / 0
MA1-744 was used in western blot to study gender differences in insulin signaling and brown adipose tissue mitochondrial biogenesis in response to diet-induced obesity in rats

Mol Cell Biochem. 2013 Jan;373(1-2):125-35.
"Sex-dependent differences in rat brown adipose tissue mitochondrial biogenesis and insulin signaling parameters in response to an obesogenic diet."
Author(s): Nadal-Casellas A, Bauzá-Thorbrügge M, Proenza AM, Gianotti M, Lladó I
Number of Citations: 0
(See PubMed article )
  Rt / 0
MA1-744 was used in western blot to characterize tyrosine phosphorylation of Gab1 in the retina

Cell Commun Signal. 2013 Mar 22;11(1):20.
"Protein tyrosine phosphatase-1B regulates the tyrosine phosphorylation of the adapter Grb2-associated binder 1 (Gab1) in the retina."
Author(s): Rajala A, Dilly AK, Rajala RV
Number of Citations: 0
(See PubMed article )
  Xl / 0
MA1-744 was used in western blot to study metabolic regulation of CaMKII protein and caspases in Xenopus

J Biol Chem. 2013 Mar 29;288(13):8838-48.
"Metabolic regulation of CaMKII protein and caspases in Xenopus laevis egg extracts."
Author(s): McCoy F, Darbandi R, Chen SI, Eckard L, Dodd K, Jones K, Baucum AJ 2nd, Gibbons JA, Lin SH, Colbran RJ, Nutt LK
Number of Citations: 0
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / 0
MA1-744 was used in immunocytochemistry to study the role of FGF-R2 in the mesenchymal-epithelial transition and bladder cancer metastasis

Cancer Res. 2006 Dec 1;66(23):11271-8.
"Mesenchymal-to-epithelial transition facilitates bladder cancer metastasis: role of fibroblast growth factor receptor-2."
Author(s): Chaffer CL, Brennan JP, Slavin JL, Blick T, Thompson EW, Williams ED
Number of Citations: 65
(See PubMed article )
  Pl / 0
MA1-744 was used in immunocytochemistry and western blot to investigate the distribution of different actin proteins in the nucleus

Cytoskeleton (Hoboken). 2010 Nov;67(11):729-43.
"Differential sublocalization of actin variants within the nucleus."
Author(s): Kandasamy MK, McKinney EC, Meagher RB
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Ms / 1:1,000
MA1-744 was used in immunohistochemistry to study the effect of Bcl-x disruption on in rod photoreceptor susceptibility to bright light stress.

Invest Ophthalmol Vis Sci. 2006 Dec;47(12):5583-9.
"Loss of BCL-XL in rod photoreceptors: Increased susceptibility to bright light stress."
Author(s): Zheng L, Anderson RE, Agbaga MP, Rucker EB 3rd, Le YZ
Number of Citations: 1
(See PubMed article )
  Pl / Not Cited
MA1-744 was used in ELISA, immunohistochemistry and western blot to study the actin-gene family of Arabidopsis in angiosperms

Plant J. 1999 Jun;18(6):681-91.
"The late pollen-specific actins in angiosperms."
Author(s): Kandasamy MK, McKinney EC, Meagher RB
Number of Citations: 14
(See PubMed article )
ELISA
  Species / Dilution Summary
  Pl / Not Cited
MA1-744 was used in ELISA, immunohistochemistry and western blot to study the actin-gene family of Arabidopsis in angiosperms

Plant J. 1999 Jun;18(6):681-91.
"The late pollen-specific actins in angiosperms."
Author(s): Kandasamy MK, McKinney EC, Meagher RB
Number of Citations: 14
(See PubMed article )
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