ATR Antibody

ATR Polyclonal Antibody for Western blot, IF, IP

>> See 7 other antibodies for ATR (Ataxia Telangiectasia Mutated (ATM) and Rad3-Related Protein)
Synonyms:
Ataxia Telangiectasia Mutated (ATM) and Rad3-Related Protein
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Human (Hu)
Immunogen: Fusion protein containing residues 400-460 of the human ATR protein.
Ordering Information
Pierce ATR Antibody
Product #
PA1-450
Size
100 µl
Price
$360.00
Purchase
Add ATR Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1,000
Immunofluorescence (IF) Assay dependent
Immunoprecipitation (IP) Assay dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunoprecipitation (IP) See publications below
ChIP assay (ChIP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 4mg/ml
Purification: Affinity chromatography
Storage Buffer: PBS
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-450 detects ATR (Ataxia Telangiectasia Mutated (ATM) and Rad3-related protein) from human cells.

PA1-450 has been successfully used in Western blot immunofluoresence and immunoprecipitation procedures. By Western blot, this antibody detects an ~305 kDa protein representing ATR in lysate from UV irradiated K562 cells.

PA1-450 antigen is a fusion protein corresponding to amino acid residues 400-460 from human ATR.

General Information
Ataxia Telangiectasia Mutated (ATM) and Rad3-related protein (ATR) is a phosphatidylinositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR is a member of the DNA-PK kinases closely related to ATM and DNA-PK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes. Several known components of the NuRD complex including histone deacetylase 1 (HDAC1), HDAC2, and CHD4.
Product Images
  • ATR Antibody (PA1-450) in WB
    PA1-450_1_07232010.jpg

    Western Blot with anti-ATR Polyclonal Antibody (PA1-450) (PA1-450_1_07232010.jpg)

    Western Blot with anti-ATR Polyclonal Antibody (PA1-450)

    Western blot on ATR in K562 whole cell lysate using PA1-450.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / 1:1,000
PA1-450 was used in immunoprecipitation and western blot to investigate the interaction of ATR with HDAC2 and CHD4

Biochemistry. 1999 Nov 2;38(44):14711-7.
"Molecular association between ATR and two components of the nucleosome remodeling and deacetylating complex, HDAC2 and CHD4."
Author(s): Schmidt DR, Schreiber SL
Number of Citations: 15
(See PubMed article )
  Hu / Not Cited
PA1-450 was used in western blot to study the caffeine-sensitivity and the relation to DNA-PK of DNA-activated protein kinase ATR

Oncogene. 1999 Nov 18;18(48):6707-13.
"ATR is a caffeine-sensitive, DNA-activated protein kinase with a substrate specificity distinct from DNA-PK."
Author(s): Hall-Jackson CA, Cross DA, Morrice N, Smythe C
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-450 was used in western blot to study the roles of checkpoint proteins on replication initiation after UV irradiation

Mol Cell Biol. 2002 Dec;22(24):8552-61.
"An ATR- and Chk1-dependent S checkpoint inhibits replicon initiation following UVC-induced DNA damage."
Author(s): Heffernan TP, Simpson DA, Frank AR, Heinloth AN, Paules RS, Cordeiro-Stone M, Kaufmann WK
Number of Citations: 42
(See PubMed article )
  Hu / Not Cited
PA1-450 was used in western blot and immunoprecipitation to study the mechanism for ATP-induced activation of ataxia-telangiectasia mutated (ATM) protein.

J Biol Chem. 2003 Mar 14;278(11):9309-17.
"ATP activates ataxia-telangiectasia mutated (ATM) in vitro. Importance of autophosphorylation."
Author(s): Kozlov S, Gueven N, Keating K, Ramsay J, Lavin MF
Number of Citations: 9
(See PubMed article )
  Hu / Not Cited
PA1-450 was used in immunoprecipitation and western blot to study how DNA damage influences the p53-dependent p21waf1 in ATM-deficient cells

Oncogene. 2003 Oct 30;22(49):7866-9.
"DNA damage-induced cell-cycle phase regulation of p53 and p21waf1 in normal and ATM-defective cells."
Author(s): Delia D, Fontanella E, Ferrario C, Chessa L, Mizutani S
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-450 was used in western blot to study ATR structure and its assocation with DNA.

Mol Cell Biol. 2004 Feb;24(3):1292-300.
"Quaternary structure of ATR and effects of ATRIP and replication protein A on its DNA binding and kinase activities."
Author(s): Unsal-Kaçmaz K, Sancar A
Number of Citations: 15
(See PubMed article )
  Hu / 0
PA1-450 was used in western blot to study the ATR-p53 pathway in normal and malignant lymphocytes

Oncogene. 2004 Mar 11;23(10):1911-21.
"The ATR-p53 pathway is suppressed in noncycling normal and malignant lymphocytes."
Author(s): Jones GG, Reaper PM, Pettitt AR, Sherrington PD
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-450 was used in western blot to study the involvement of ATR-Chk1 pathway in the inhibitory effect of 6-thioguanine on DNA mismatch repair

Biochem Biophys Res Commun. 2004 May 21;318(1):297-302.
"Mismatch repair-mediated G2/M arrest by 6-thioguanine involves the ATR-Chk1 pathway."
Author(s): Yamane K, Taylor K, Kinsella TJ
Number of Citations: 12
(See PubMed article )
  Hu / Not Cited
PA1-450 was used in western blot to investigate the role of replication protein A during the DNA replication.

J Biol Chem. 2004 Aug 6;279(32):34010-4.
"DNA replication defects, spontaneous DNA damage, and ATM-dependent checkpoint activation in replication protein A-deficient cells."
Author(s): Dodson GE, Shi Y, Tibbetts RS
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-450 was used in western blot to investigate the function of proteins from phosphoinositide-3-kinase-related family

Methods Mol Biol. 2004;281():125-41.
"Determination of the catalytic activities of mTOR and other members of the phosphoinositide-3-kinase-related kinase family."
Author(s): Chiang GG, Abraham RT
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-450 was used in western blot to study the role of hMCM7 in the DNA damage signal transmission of from active replication forks to the S-phase checkpoint.

EMBO J. 2004 Nov 24;23(23):4660-9.
"Interaction between human MCM7 and Rad17 proteins is required for replication checkpoint signaling."
Author(s): Tsao CC, Geisen C, Abraham RT
Number of Citations: 1
(See PubMed article )
  Hu / 0
PA1-450 was used in western blot to investigate the involvement of DNA damage machinery and homologous recombination pathway in the maintenance of telomere integrity

Cell. 2006 Nov 17;127(4):709-20.
"The DNA damage machinery and homologous recombination pathway act consecutively to protect human telomeres."
Author(s): Verdun RE, Karlseder J
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-450 was used in immunoprecipitation and western blot to investigate the role of p300/CBP acetyltransferases for the DNA replication checkpoint

J Biol Chem. 2007 Mar 30;282(13):9678-87.
"p300/CREB-binding protein interacts with ATR and is required for the DNA replication checkpoint."
Author(s): Stauffer D, Chang B, Huang J, Dunn A, Thayer M
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-450 was used in western blot to investigate the role of BRCA1 in the G (2)-M checkpoint response to 6-thioguanine-induced DNA mismatch damage

Cancer Res. 2007 Jul 1;67(13):6286-92.
"BRCA1 activates a G2-M cell cycle checkpoint following 6-thioguanine-induced DNA mismatch damage."
Author(s): Yamane K, Schupp JE, Kinsella TJ
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-450 was used in western blot to evaluate an immortalized human neural stem-cell line as an in vitro model for the study of neuronal DNA-damage response

Cell Death Differ. 2009 Jun;16(6):795-806.
"DNA-damage response, survival and differentiation in vitro of a human neural stem cell line in relation to ATM expression."
Author(s): Carlessi L, De Filippis L, Lecis D, Vescovi A, Delia D
Number of Citations: 2
(See PubMed article )
  Hu / Not Cited
PA1-450 was used in ChIP assay and western blot to investigate the interaction between ATR and common fragile site FRA3B and its effect on the regulation of fragile site stability

Mutat Res. 2010 Apr 1;686(1-2):39-46.
"ATR preferentially interacts with common fragile site FRA3B and the binding requires its kinase activity in response to aphidicolin treatment."
Author(s): Wan C, Kulkarni A, Wang YH
Number of Citations: 3
(See PubMed article )
  Hu / Not Cited
PA1-450 was used in western blot to study the role of casein kinase 1 in checkpoint responses to stalled DNA replication forks

Mol Biol Cell. 2011 Aug 15;22(16):2834-47.
"Role for casein kinase 1 in the phosphorylation of Claspin on critical residues necessary for the activation of Chk1."
Author(s): Meng Z, Capalbo L, Glover DM, Dunphy WG
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
PA1-450 was used in immunoprecipitation and western blot to investigate the interaction of ATR with HDAC2 and CHD4

Biochemistry. 1999 Nov 2;38(44):14711-7.
"Molecular association between ATR and two components of the nucleosome remodeling and deacetylating complex, HDAC2 and CHD4."
Author(s): Schmidt DR, Schreiber SL
Number of Citations: 15
(See PubMed article )
  Hu / Not Cited
PA1-450 was used in western blot and immunoprecipitation to study the mechanism for ATP-induced activation of ataxia-telangiectasia mutated (ATM) protein.

J Biol Chem. 2003 Mar 14;278(11):9309-17.
"ATP activates ataxia-telangiectasia mutated (ATM) in vitro. Importance of autophosphorylation."
Author(s): Kozlov S, Gueven N, Keating K, Ramsay J, Lavin MF
Number of Citations: 9
(See PubMed article )
  Hu / Not Cited
PA1-450 was used in immunoprecipitation and western blot to study how DNA damage influences the p53-dependent p21waf1 in ATM-deficient cells

Oncogene. 2003 Oct 30;22(49):7866-9.
"DNA damage-induced cell-cycle phase regulation of p53 and p21waf1 in normal and ATM-defective cells."
Author(s): Delia D, Fontanella E, Ferrario C, Chessa L, Mizutani S
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-450 was used in immunoprecipitation to study the interaction between MMR proteins and ATR during the cellular response to genotoxic stress.

EMBO J. 2004 Aug 4;23(15):3164-74.
"ATR functions as a gene dosage-dependent tumor suppressor on a mismatch repair-deficient background."
Author(s): Fang Y, Tsao CC, Goodman BK, Furumai R, Tirado CA, Abraham RT, Wang XF
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-450 was used in immunoprecipitation and western blot to investigate the role of p300/CBP acetyltransferases for the DNA replication checkpoint

J Biol Chem. 2007 Mar 30;282(13):9678-87.
"p300/CREB-binding protein interacts with ATR and is required for the DNA replication checkpoint."
Author(s): Stauffer D, Chang B, Huang J, Dunn A, Thayer M
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-450 was used in immunoprecipitation to study the role of FANCA phosphorylation in Fanconi anemia.

Blood. 2009 Mar 5;113(10):2181-90.
"ATR-dependent phosphorylation of FANCA on serine 1449 after DNA damage is important for FA pathway function."
Author(s): Collins NB, Wilson JB, Bush T, Thomashevski A, Roberts KJ, Jones NJ, Kupfer GM
Number of Citations: 1
(See PubMed article )
ChIP assay
  Species / Dilution Summary
  Hu / Not Cited
PA1-450 was used in ChIP assay and western blot to investigate the interaction between ATR and common fragile site FRA3B and its effect on the regulation of fragile site stability

Mutat Res. 2010 Apr 1;686(1-2):39-46.
"ATR preferentially interacts with common fragile site FRA3B and the binding requires its kinase activity in response to aphidicolin treatment."
Author(s): Wan C, Kulkarni A, Wang YH
Number of Citations: 3
(See PubMed article )
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