100 ul of antibody in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol.
Western Blot (WB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-17398 detects AMPK-alpha1 in human and non-human primate samples. Predicted to react with porcine based on sequence homology.
PA5-17398 has been successfully used in Western blot applications.
The PA5-17398 immunogen is synthetic peptide corresponding to residues surrounding Leu519 near the carboxy terminus of human AMPK-alpha-1.
PA5-17398 detects AMPK-alpha1 which has a predicted molecular weight of approximately 62 kDa.
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
AMPK-alpha1 Antibody (PA5-17398) in WB
Western Blot with anti-AMPK-alpha1 Polyclonal Antibody (PA5-17398)