AKT1 Antibody

(This product has been discontinued.)
Please click the link below to find suitable replacements for this discontinued product.

>> See 30 other antibodies for AKT1

AKT1 Monoclonal Antibody for Western blot

>> See 30 other antibodies for AKT1 (PKB, RAC, PRKBA)
Synonyms:
PKB, RAC, PRKBA
Entrez Gene ID:
Details
Host / Isotype: Mouse / IgG
Class: Monoclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Immunogen: v-akt-1, Protein kinase B
Ordering Information
Pierce AKT1 Antibody
Product #
82310
Size
100 ul
Price
$264.00
Purchase
(DISCONTINUED)
Tested Applications Dilution *
Western Blot (WB) 1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form information is not available for this product.
Product Specific Information
82310 targets AKT1 in WB applications and shows reactivity with Human, mouse, and Rat samples.

The 82310 immunogen is v-akt-1, Protein kinase B.

82310 detects AKT1 which has a predicted molecular weight of approximately 60 kDa.
General Information
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Product Images
  • AKT1 Antibody (82310) in WB
    82310.jpg

    Western Blot with anti-AKT1 Monoclonal Antibody (82310) (82310.jpg)

    Western Blot with anti-AKT1 Monoclonal Antibody (82310)

    A549 cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ON-TARGETplus AKT1 siRNA (Lane 3). Western blot data for GAPDH is included as a control for equal protein loading.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific