* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
This antibody is predicted to react with mouse, porcine and rat based on sequence homology.
ADCYAP1 is adenylate cyclase activating polypeptide 1. Mediated by adenylate cyclase activating polypeptide 1 receptors, this polypeptide stimulates adenylate cyclase and subsequently increases the cAMP level in target cells. Adenylate cyclase activating polypeptide 1 is not only a hypophysiotropic hormone, but also functions as a neurotransmitter and neuromodulator. In addition, it plays a role in paracrine and autocrine regulation of certain types of cells. This gene encodes three different mature peptides, including two isotypes, a shorter form and a longer form.
ADCYAP1 Antibody (PA5-25575) in WB ADCYAP1-_Antibody_PA5-25575_WB_1_112013.jpg
Western Blot with anti-ADCYAP1 Polyclonal Antibody (PA5-25575)
Immunofluorescent analysis of 293 cells using an ADCYAP1 polyclonal antibody (Product # PA5-25575) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).
ADCYAP1 Antibody (PA5-25575) in IHC ADCYAP1-_Antibody_PA5-25575_IHC_1_112013.jpg
Immunohistochemistry with anti-ADCYAP1 Polyclonal Antibody (PA5-25575)
Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human testis tissue using an ADCYAP1 polyclonal antibody (Product # PA5-25575), followed by HRP-conjugated secondary antibody and DAB staining.
ADCYAP1 Antibody (PA5-25575) in FACS ADCYAP1-_Antibody_PA5-25575_FC_1_112013.jpg
Flow Cytometry with anti-ADCYAP1 Polyclonal Antibody (PA5-25575)
Flow cytometry analysis of 293 cells using an ADCYAP1 polyclonal antibody (Product # PA5-25575) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
(This product is for In Vitro experimental use only. Not for resale without express authorization.)