|Host / Isotype:||Rabbit|
|Tested Species Reactivity:||Human (Hu), Bovine (Bv), Porcine (Po)|
|Published Species Reactivity:||Human (Hu)|
|Immunogen:||Synthetic peptide corresponding to residues C V(394) M A H V D P E E P(403) of mouse ADAMTS4.|
|Pierce ADAMTS4 Antibody|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 ug/ml|
|Western Blot (WB)||See publications below|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
|Storage Buffer:||PBS with 1mg/ml BSA|
|Preservative:||0.05% sodium azide|
|Storage Conditions:||-20° C, Avoid Freeze/Thaw Cycles|
|Product Specific Information|
|PA1-1749A detects ADAMTS4 from porcine, bovine, and human samples. PA1-1749A is specific for ADAMTS4 and shows no cross-reactivity with other ADAMTS family members.
PA1-1749A has been successfully used in Western blot procedures. By Western blot, this antibody detects ADAMTS4 at ~68, ~53 and 30 kDa protein representing ADAMTS4 and its proteolytic fragments. Depending on the extent of C-terminal processing, several smaller bands may also be seen using this antibody.
PA1-1749A immunogen is a synthetic peptide corresponding to residues C V(394) M A H V D P E E P(403) of mouse ADAMTS4. PA1-1749A immunizing peptide (Cat. # PEP-234) is available for use in neutralization and control procedures. This polyclonal antibody has been referred to as: JSCVMA. PA1-1749A has been used in combination with PA1-1750 for detection of all ADAMTS4 processed forms.
|A disintegrin-like and metalloproteinase thrombospondin type 1 motif 4 (ADAMTS4 or aggrecanase 1) is a member of the ADAMTS family of multidomain proteins that has demonstrated metalloproteinase functions. The ADAMTS4 protein contains a prohormone convertase cleavage site, thrombospondin-1 like domain, cysteine-rich domain, and a propeptide domain. ADAMTS4 activity had been shown to be blocked by TIMP-1, 2, 3, and 4.
ADAMTS4 plays an important role in the cleavage of aggrecan, versican and brevican. Studies have determined that aggrecan is cleaved within the interglobular domain of aggrecan at sites Glu(373)-Ala(374) and Phe(342)-Glu(373). Aggrecan degradation has been linked to the development of rheumatoid arthritis, osteoarthritis, retinal diseases, Alzheimer’s and other neurodegenerative diseases.
|Species / Dilution||Summary|
Hu / Not Cited
PA1-1749A was used in western blot to investigate the involvement of ADAMTS-4 in the anti-atherogenic effect of transforming growth factor
Int J Biochem Cell Biol. 2011 May;43(5):805-11.
"The expression of a disintegrin and metalloproteinase with thrombospondin motifs 4 in human macrophages is inhibited by the anti-atherogenic cytokine transforming growth factor-? and requires Smads, p38 mitogen-activated protein kinase and c-Jun."
Author(s): Salter RC, Arnaoutakis K, Michael DR, Singh NN, Ashlin TG, Buckley ML, Kwan AP, Ramji DP
Number of Citations: 2
(See PubMed article )