Part of Thermo Fisher Scientific

ABCA7 Monoclonal Antibody (7A1-144)

ABCA7 Antibody for FACS, Western blot

Synonyms:
ABC 1 antibody, ABC Transporter 1 antibody, ABC1 antibody, ATP-binding Cassette 1 antibody, ATP-binding Cassette Transporter 1 antibody, CERP antibody
Entrez Gene ID:
(Murine) 10347, (Murine) 27403
UniProt ID:
Q8IZY2
Details
Host / Isotype: Mouse / IgG2a, kappa
Class: Monoclonal
Type: Antibody
Clone: 7A1-144
Species Reactivity: Murine (Ms)
Immunogen: Mouse ABCA7 stably-transfected HeLa cells.
Ordering Information
Pierce ABCA7 Monoclonal Antibody (7A1-144)
Product Number Pkg. Size Price Purchase
MA1-16935 100 µl $262.00
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Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 µl of protein G purified rat ascites in Tris-glycice with 150mM NaCl containing 0.05% sodium azide.


Applications Dilution *
Flow Cytometry (FACS) 1:500
Western Blot (WB) 1:500
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA1-16935 detects ABCA7 protein in mouse samples.

MA1-16935 has been successfully used in flow cytometry and Western blot procedures. By Western blot, MA1-16935 is specific for the ~240 kDa ABCA7 protein.

MA1-16935 immunogen is mouse ABCA7 stably-transfected HeLa cells.

Figure 1 shows FACS analysis of 6C (red) (negative control HeLa cells) and A7 (blue) (ABCA7 expressing HeLa cells) using MA1-16935 at a 1:2,000 dilution.

Figure 2 shows a Western blot detection of ABCA7 using MA1-16935 at various dilutions in ABCA7 transfected HeLa lysates.
General Information
Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.
Product Images
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  • WB using MA1-16935
    Western Blot with anti-ABCA7 Monoclonal Antibody [7A1-144] (MA1-16935)

    Western Blot with anti-ABCA7 Monoclonal Antibody [7A1-144] (MA1-16935)

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  • WB using MA1-16935
    Western Blot with anti-ABCA7 Monoclonal Antibody [7A1-144] (MA1-16935)

    Western Blot with anti-ABCA7 Monoclonal Antibody [7A1-144] (MA1-16935)

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    Detection of ABCA7 using MA1-16935 at various dilutions in ABCA7 transfected HeLa lysates.
     
  • FACS using MA1-16935
    Flow Cytometry with anti-ABCA7 Monoclonal Antibody [7A1-144] (MA1-16935)

    Flow Cytometry with anti-ABCA7 Monoclonal Antibody [7A1-144] (MA1-16935)

    View Product Details >>
    6C (red) (negative control HeLa cells) and A7 (blue) (ABCA7 expressing HeLa cells) using MA1-16935 at a 1:2,000 dilution.
     
 
 
(This product is for In Vitro experimental use only. Not for resale without express authorization.)