Part of Thermo Fisher Scientific

ABCA1 Monoclonal Antibody (3A1.891.3)

ABCA1 Antibody for Western blot

Synonyms:
ABC 1 antibody, ABC Transporter 1 antibody, ABC1 antibody, ATP-binding Cassette 1 antibody, ATP-binding Cassette Transporter 1 antibody, CERP antibody
Entrez Gene ID:
(Murine) 19, (Murine) 11303
UniProt ID:
O95477
Details
Host / Isotype: Rat / IgG1, kappa
Class: Monoclonal
Type: Antibody
Clone: 3A1.891.3
Species Reactivity: Murine (Ms)
Immunogen: Synthetic peptide corrseponding to the N-terminal loop residues 200-250 of mouse ABCA1.
Ordering Information
Pierce ABCA1 Monoclonal Antibody (3A1.891.3)
Product Number Pkg. Size Price Purchase
MA1-16936 100 µl $346.00
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Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 µl of diluted ascites containing 0.1% sodium azide.


Applications Dilution *
Western Blot (WB) 1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA1-16936 detects ABCA1 protein in mouse samples. This antibody does not react with human ABCA1.

MA1-16936 has been successfully used in Western blot procedures. By Western blot, MA1-16936 is specific for the ~250 kDa ABCA1 protein. Important note: ABCA1 is known to aggregate. DO NOT BOIL SAMPLES. Use a reducing buffer: 2X sample buffer: 8M Urea, 2% SDS, 715 mM BME, 1% glycerol, 0.001% Bromophenol Blue, 125 mM Tris-HCl [pH 6.8].

The MA1-16936 immunogen is a synthetic peptide corrseponding to the N-terminal loop residues 200-250 of mouse ABCA1.

Figure 1 shows a Western blot detection of ABCA1 using MA1-16936 at various dilutions in ABCA1 transfected HeLa lysates.
General Information
Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.
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  • WB using MA1-16936
    Western Blot with anti-ABCA1 Monoclonal Antibody [3A1.891.3] (MA1-16936)

    Western Blot with anti-ABCA1 Monoclonal Antibody [3A1.891.3] (MA1-16936)

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  • WB using MA1-16936
    Western Blot with anti-ABCA1 Monoclonal Antibody [3A1.891.3] (MA1-16936)

    Western Blot with anti-ABCA1 Monoclonal Antibody [3A1.891.3] (MA1-16936)

    View Product Details >>
    Detection of ABCA1 using MA1-16936 at various dilutions in ABCA1 transfected HeLa lysates.
     
 
 
(This product is for In Vitro experimental use only. Not for resale without express authorization.)