Part of Thermo Fisher Scientific

AAT Monoclonal Antibody (2B12)

AAT Antibody for ELISA, Western blot

Synonyms:
PI; A1A; AAT; PI1; A1AT; MGC9222; PRO2275; MGC23330; SERPINA1
Entrez Gene ID:
(Human) 5265
UniProt ID:
P01009
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 2B12
Species Reactivity: Human (Hu) Murine (Ms)
Immunogen: Purified recombinant fragment of human AAT expressed in E. Coli.
Ordering Information
Pierce AAT Monoclonal Antibody (2B12)
Product Number Pkg. Size Price Purchase
MA5-15521 100 ul $299.00
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Storage: Store at 4º, for long term storage, store at -20º.
Form: 100 ul of ascitic fluid containing 0.03% sodium azide.


Applications Dilution *
Western Blot (WB) 1/500 - 1/2000
ELISA (ELISA) 1/10000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA5-15521 targets AAT in ELISA and WB applications and shows reactivity with Human and Murine samples.

The MA5-15521 immunogen is purified recombinant fragment of human AAT expressed in E. Coli.

MA5-15521 detects AAT which has a predicted molecular weight of approximately 47kDa.
General Information
The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.
Product Images
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  • WB using MA5-15521
    Western Blot with anti-AAT Monoclonal Antibody [2B12] (MA5-15521)

    Western Blot with anti-AAT Monoclonal Antibody [2B12] (MA5-15521)

    View Product Details >>
    Western blot analysis using AAT antibody against human plasma (1) and NIH/3T3 cell lysate (2).
     
 
 
References:
J Proteome Res. 2005 Nov-Dec;4(6):2070-80.
Neuro Endocrinol Lett. 2008 Aug;29(4):482-4.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)