6x-His Epitope Tag Antibody (HIS.H8)

6x-His Epitope Tag Monoclonal Antibody for Western blot, IF, IP, ELISA

Details
Host / Isotype: Mouse / IgG2b
Class: Monoclonal
Type: Antibody
Clone: HIS.H8
Tested Species Reactivity: Tag (Tag)
Published Species Reactivity: Not Applicable (N/A)
Immunogen: 6x His synthetic peptide.
Ordering Information
Pierce 6x-His Epitope Tag Antibody (HIS.H8)
Product #
MA1-21315
Size
100 µg
Price
$149.00
Purchase
Add 6x-His Epitope Tag Antibody (HIS.H8) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000 - 1:3000
Immunofluorescence (IF) 1:500 - 1:2000
Immunoprecipitation (IP) Assay dependent
ELISA (ELISA) Assay dependent
Published Applications Dilution
Western Blot (WB) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Protein A
Storage Buffer: PBS, pH 7.2
Preservative: no preservative
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
MA1-21315 detects 6X-His Epitope Tag in samples.

MA1-21315 has been successfully used in ELISA, immunofluorescence, immunoprecipitation and Western Blot procedures.

MA1-21315 immunogen corresponds to 6x His synthetic peptide.

MA1-21315 may be stored at 4ºC short term. Add 0.05% sodium azide if desired. For long term storage, store at -20ºC, avoiding freeze/thaw cycles.
General Information
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Expression of recombinant proteins in E. coli as a fusion protein with neighboring histidine residues is one of the most popular methods of epitope tags. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent.
Product Images
  • 6x-His Epitope Tag Antibody (MA1-21315) in WB
    MA1-21315_WesternBlot.jpg

    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315) (MA1-21315_WesternBlot.jpg)

    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315)

    Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5ug/mL)
  • 6x-His Epitope Tag Antibody (MA1-21315) in WB
    MA1-21315_WesternBlot_2.jpg

    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315) (MA1-21315_WesternBlot_2.jpg)

    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315)

    Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2)
  • 6x-His Epitope Tag Antibody (MA1-21315) in WB
    MA121315 figure for website.jpg

    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315) (MA121315 figure for website.jpg)

    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315)

    Western blot analysis of His-tagged protein in E. coli lysates. Membranes containing total cell lysates from uninduced E. coli (-) or induced E. coli (+) treated with 1mM Isopropyl beta-D-1-thiogalactopyranoside (IPTG) to induce expression of His-tagged IQ domain (IQD) protein were blocked with 5% non-fat milk in PBS-T and probed with a 6X-His Epitope Tag Antibody (Product # MA1-21315) at a dilution of 1:1400 followed by a donkey anti-mouse IgG-HRP secondary antibody. Blots were developed with SuperSignal West Dura (Product # 34076). His-tagged Repressor of Gibberellin (RGA) was used as a positive control. Data courtesy of the Innovators Program.
  • 6x-His Epitope Tag Antibody (MA1-21315) in IF
    MA1-21315_Immunofluorescence.jpg

    Immunofluorescence with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315) (MA1-21315_Immunofluorescence.jpg)

    Immunofluorescence with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315)

    LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)
Publications:
Western Blot
  Species / Dilution Summary
  N/A / 0
MA1-21315 was used in western blot to study whether the recombinant C-terminal olfactomedin domain of myocilin affects outflow facility in porcine eyes

Invest Ophthalmol Vis Sci. 2003 May;44(5):1953-61.
"Perfusion with the olfactomedin domain of myocilin does not affect outflow facility."
Author(s): Goldwich A, Ethier CR, Chan DW, Tamm ER
Number of Citations: 8
(See PubMed article )
  N/A / 0
MA1-21315 was used in western blot to study Mcm10 homocomplex assembly

J Biol Chem. 2003 Sep 19;278(38):36051-8.
"A novel zinc finger is required for Mcm10 homocomplex assembly."
Author(s): Cook CR, Kung G, Peterson FC, Volkman BF, Lei M
Number of Citations: 13
(See PubMed article )
  N/A / 1:1000
MA1-21315 was used in western blot to study the interaction between SUMO and vestigial and its role in fly wing development

Mech Dev. 2005 Oct;122(10):1130-7.
"SUMO enhances vestigial function during wing morphogenesis."
Author(s): Takanaka Y, Courey AJ
Number of Citations: 3
(See PubMed article )
  N/A / 0
MA1-21315 was used in western blot to biochemically characterize diacylglycerol kinase AtDGK7 of Arabadopsis thaliana

J Biol Chem. 2005 Oct 14;280(41):34888-99.
"Arabidopsis AtDGK7, the smallest member of plant diacylglycerol kinases (DGKs), displays unique biochemical features and saturates at low substrate concentration: the DGK inhibitor R59022 differentially affects AtDGK2 and AtDGK7 activity in vitro and alters plant growth and development."
Author(s): Gómez-Merino FC, Arana-Ceballos FA, Trejo-Téllez LI, Skirycz A, Brearley CA, Dörmann P, Mueller-Roeber B
Number of Citations: 15
(See PubMed article )
  N/A / 1:500
MA1-21315 was used in western blot to study the P2X receptor ion channel ion access pathway

J Gen Physiol. 2011 Jun;137(6):579-90.
"Ion access pathway to the transmembrane pore in P2X receptor channels."
Author(s): Kawate T, Robertson JL, Li M, Silberberg SD, Swartz KJ
Number of Citations: 19
(See PubMed article )
  N/A / 1:1000
MA1-21315 was used in western blot to characterize SUMO cycle components in Hydra

Genesis. 2013 Sep;51(9):619-29.
"The Hydra small ubiquitin-like modifier."
Author(s): Khan U, Mehere P, Deivasigamani S, Ratnaparkhi GS
Number of Citations: 0
(See PubMed article )
  N/A / 0
MA1-21315 was used in western blot to express and purify the nucleocapsid protein of Schmallenberg virus, and raise a monclonal antibody against it

Protein Expr Purif. 2013 Nov;92(1):1-8.
"Expression and purification of the nucleocapsid protein of Schmallenberg virus, and preparation and characterization of a monoclonal antibody against this protein."
Author(s): Zhang Y, Wu S, Wang J, Wernike K, Lv J, Feng C, Zhang J, Wang C, Deng J, Yuan X, Lin X
Number of Citations: 0
(See PubMed article )
  N/A / 1:2500
MA1-21315 was used in western blot to study the ability of Escherchia coli YmdB to regulate biofilm formation independently of ribonuclease III

BMC Microbiol. 2013 Nov 24;13():266.
"Escherichia coli YmdB regulates biofilm formation independently of its role as an RNase III modulator."
Author(s): Kim T, Lee J, Kim KS
Number of Citations: 0
(See PubMed article )
  N/A / 0
MA1-21315 was used in western blot to establish a process for producing HIV-1 capsid protein p24 in E.coli

BMC Biotechnol. 2013 Dec 4;13():107.
"Production and purification of polymerization-competent HIV-1 capsid protein p24 (CA) in NiCo21(DE3) Escherichia coli."
Author(s): Teow SY, Mualif SA, Omar TC, Wei CY, Yusoff NM, Ali SA
Number of Citations: 0
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific