Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Form:
100 µg (0.25 mg/ml) of Protein A and immunogen affinity purified antibody supplied in PBS with 0.09% (W/V) sodium azide. Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applications
Dilution *
Western Blot (WB)
1:50-100
Flow Cytometry (FACS)
1:10-50
Immunohistochemistry (IHC)
1:50-100
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-26699 targets KRT1 in FACS, IHC, and WB applications and shows reactivity with human samples.
The PA5-26699 immunogen is synthetic peptide from the Central region of human KRT1 conjugated to KLH.
General Information
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides.
Product Images
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KRT1 Antibody (PA5-26699) in FACS
Flow Cytometry with anti-KRT1 Polyclonal Antibody (PA5-26699)
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis in formalin fixed and paraffin embedded skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.