Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
100 ug of antibody purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS and supplied in PBS with 0.09% (W/V) sodium azide.
Western Blot (WB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-26597 targets CHAT in IF and WB applications and shows reactivity with Human and Murine samples.
The PA5-26597 immunogen is synthetic peptide from the N-terminal region of human CHAT conjugated to KLH.
Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).
CHAT Antibody (PA5-26597) in WB
Western Blot with anti-CHAT Polyclonal Antibody (PA5-26597)