Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Form:
100 ug of antibody purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS and supplied in PBS with 0.09% (W/V) sodium azide.
Applications
Dilution *
Western Blot (WB)
1:50-100
Immunohistochemistry (IHC)
1:50-100
Flow Cytometry (FACS)
1:10-50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-26382 targets PLCB4 in FACS, IHC, and WB applications and shows reactivity with Human and Murine samples.
The PA5-26382 immunogen is synthetic peptide from the C-terminal region of human PLCB4 conjugated to KLH.
General Information
Product Images
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PLCB4 Antibody (PA5-26382) in FACS
Flow Cytometry with anti-PLCB4 Polyclonal Antibody (PA5-26382)
Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis in formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated to the secondary antibody, followed by DAB staining.